Human asialoglyco protein receptor,ASGPR ELISA Kit

Code CSB-E09130h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name asialoglyco protein receptor,ASGPR
Alternative Names ASGP-R 1 ELISA Kit; ASGPR 1 ELISA Kit; ASGPR ELISA Kit; ASGPR1 ELISA Kit; Asgr1 ELISA Kit; ASGR1_HUMAN ELISA Kit; Asialoglycoprotein receptor 1 ELISA Kit; C type lectin domain family 4 member H1 ELISA Kit; C-type lectin domain family 4 member H1 ELISA Kit; CLEC4H1 ELISA Kit; Hepatic lectin H1 ELISA Kit; HL-1 ELISA Kit
Abbreviation ASGPR
Uniprot No. P07306
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.2 ng/mL-2000 ng/mL
Sensitivity 7.8 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ASGPR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %90-102
1:2Average %95
Range %83-101
1:4Average %96
Range %90-106
1:8Average %94
Range %82-101
Recovery
The recovery of human ASGPR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9289-98
EDTA plasma (n=4)9491-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
20002.705 2.618 2.662 2.550
10001.987 2.040 2.014 1.902
5001.359 1.341 1.350 1.238
2500.786 0.762 0.774 0.662
1250.472 0.458 0.465 0.353
62.50.318 0.307 0.313 0.201
31.20.181 0.186 0.184 0.072
00.109 0.115 0.112  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.
Gene References into Functions
  1. Authors report the generation of a human embryonic stem cell line WAe001-A-6 harbouring homozygous ASGR1 mutations using CRISPR/Cas9. The mutation involves a 37bp deletion, resulting in a frame shift. PMID: 28952928
  2. PEGylated lipoplexes were well tolerated by both HEK293 (ASGP-R-negative) and HepG2 (ASGP-R-positive) cell lines and delivered DNA to the hepatoma cell line HepG2 by ASGP-R mediation at levels three-fold greater than nonPEGylated lipoplexes. PMID: 28063265
  3. findings show that the hepatitis E virus (HEV) ORF2 protein interacts directly with the ectodomain of both ASGR1 and ASGR2; ASGPR is involved in and facilitates HEV infection by binding to ORF2 PMID: 27155063
  4. ASGR1 can inhibit the activity of V-ATPase by interacting with LASS2, thereby suppressing the metastatic potential of hepatoma cells. PMID: 27241665
  5. This review will focus on the mechanisms of platelet senescence with specific emphasis on the role of post-translational modifications in platelet life-span and thrombopoietin production downstream of the hepatic Ashwell-Morrell receptor, originally termed asialoglycoprotein receptor (ASGPR). PMID: 27207430
  6. ASGR1 haploinsufficiency was associated with reduced levels of non-HDL cholesterol and a reduced risk of coronary artery disease. PMID: 27192541
  7. Flow cytometric assessment of ASGPR expression may be a useful predictor of liver dysfunction following major hepatectomy for HCC in Chinese patients. PMID: 25404440
  8. Anti-ASGPR antibody could be used for specific and efficient HCC CTC enrichment, and anti-P-CK combined with anti-CPS1 antibodies is superior to identification with one antibody alone in the sensitivity for HCC CTC detection. PMID: 24763545
  9. Endocytic AMR controls TPO expression through Janus kinase 2 (JAK2) and the acute phase response signal transducer and activator of transcription 3 (STAT3) in vivo and in vitro PMID: 25485912
  10. Anti-ASGPR levels correlate with biochemical parameters and with the severity and manifestation autoimmune processes in patients with autoimmune hepatitis. PMID: 24933948
  11. The examined ASGPR1 expression levels by immunohistochemistry in HCC with different grades. Guidance for a targeting delivery strategy for anti-cancer drugs to HCC is suggested in this report. PMID: 23979840
  12. the distribution of ASGR in human testis, was investigated. PMID: 23604802
  13. the cooperative binding mode of Ca(2+) makes it possible for ASGP-R to be more sensitive to Ca(2+) concentrations in early endosomes, and plays an important role in the efficient release of ligand from ASGP-R PMID: 22613667
  14. Constant sH2a levels suggest constitutive secretion from hepatocytes in healthy individuals; therefore, a decrease with cirrhosis suggests a diagnostic potential. PMID: 22219600
  15. found that the asialoglycoprotein receptor (ASGPR) is involved in hepatocyte recognition of cells predestined for killing, including activated autologous T lymphocytes PMID: 21656538
  16. Asialoglycoprotein receptor (ASGPR) interacted specifically and directly with the preS1 domain of HBV in vivo and in vitro. PMID: 21207081
  17. Two naturally occurring ASGPR1 splice variants are produced in human hepatocytes. PMID: 20886072
  18. our findings suggest that both fibronectin and ASGPR mediate HBsAg binding to the cell surface, which provides further evidence for the potential roles of these two proteins in mediating HBV binding to liver cells. PMID: 20364278
  19. The minor subunit splice variants, H2b and H2c, of the human asialoglycoprotein receptor are present with the major subunit H1 in different hetero-oligomeric receptor complexes PMID: 11943787
  20. primary renal proximal tubular epithelial cells have a functional ASGPR, consisting of the H1 and H2 subunits, that is capable of specific ligand binding and uptake PMID: 12119473
  21. Phosphorylation-dependent interaction with molecular chaperones PMID: 12167617
  22. the effects of palmitoylation on ASGP-R activity and function PMID: 12359251
  23. the spacing of a Cys residue relative to the TMD in the primary protein sequence of H1 is the major determinant for successful palmitoylation PMID: 12370180
  24. Exposure of beta-galactose results in the rapid clearance of platelets from the circulation by asialoglycoprotein receptor-expressing liver macrophages and hepatocytes. PMID: 19520807

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Subcellular Location [Isoform H1a]: Membrane; Single-pass type II membrane protein.; [Isoform H1b]: Secreted.
Tissue Specificity Expressed exclusively in hepatic parenchymal cells.
Database Links

HGNC: 742

OMIM: 108360

KEGG: hsa:432

STRING: 9606.ENSP00000269299

UniGene: Hs.12056

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