Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

ASGR1

Uniprot No.

P07306

Research Area

Signal Transduction

Alternative Names

ASGP-R 1; ASGPR 1; ASGPR; ASGPR1; Asgr1; ASGR1_HUMAN; Asialoglycoprotein receptor 1; C type lectin domain family 4 member H1; C-type lectin domain family 4 member H1; CLEC4H1; Hepatic lectin H1; HL-1

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

68-291aa

Target Protein Sequence

EELRGLRETFSNFTASTEAQVKGLSTQGGNVGRKMKSLESQLEKQQKDLSEDHSSLLLHVKQFVSDLRSLSCQMAALQGNGSERTCCPVNWVEHERSCYWFSRSGKAWADADNYCRLEDAHLVVVTSWEEQKFVQHHIGPVNTWMGLHDQNGPWKWVDGTDYETGFKNWRPEQPDDWYGHGLGGGEDCAHFTDDGRWNDDVCQRPYRWVCETELDKASQEPPLL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

29.7kDa

Protein Length

Extracellular Domain

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The generation of the recombinant human ASGR1 protein begins by cloning the ASGR1 gene fragment (68-291aa) with the N-terminal 6xHis-tag gene into an expression vector. The recombinant vectors are then transformed into E. coli cells, which are cultured for protein expression upon induction with IPTG. The cells are lysed to release the expressed ASGR1 protein, which is subsequently purified using Ni-NTA affinity chromatography, capitalizing on the 6xHis tag's affinity for nickel ions. The eluted ASGR1 protein is then analyzed for purity using SDS-PAGE, which demonstrates a purity exceeding 90%.

The Human ASGR1 is a critical component of the hepatic system, primarily expressed on the surface of hepatocytes [5]. It functions as a lectin that specifically recognizes and binds to asialoglycoproteins, which are glycoproteins lacking sialic acid residues. This receptor plays a significant role in the clearance of these glycoproteins from the bloodstream, facilitating their internalization through clathrin-mediated endocytosis [1][2]. The ASGR1 is part of a larger family of receptors, which includes ASGPR2, and together they are essential for maintaining the homeostasis of glycoproteins in the liver [3][4].

ASGR1's expression is not only crucial for normal liver function but also has implications in various pathological conditions, including liver diseases and cancers. Studies have shown that ASGR1 expression can be downregulated in hepatocellular carcinoma (HCC), suggesting its potential utility as a biomarker for tumor progression [6][7].

ASGR1 has been implicated in the uptake of HBV, indicating its role in viral pathogenesis [8][9]. The receptor's interaction with HBV suggests that targeting ASGR1 could be a viable strategy for developing antiviral therapies.

References:
[1] M. Tanowitz, L. Hettrick, A. Revenko, G. Kinberger, T. Prakash, & P. Seth, Asialoglycoprotein receptor 1 mediates productive uptake of n-acetylgalactosamine-conjugated and unconjugated phosphorothioate antisense oligonucleotides into liver hepatocytes, Nucleic Acids Research, vol. 45, no. 21, p. 12388-12400, 2017. https://doi.org/10.1093/nar/gkx960
[2] W. Xu, L. Cao, L. Chen, J. Li, X. Zhang, H. Qian, et al. Isolation of circulating tumor cells in patients with hepatocellular carcinoma using a novel cell separation strategy, Clinical Cancer Research, vol. 17, no. 11, p. 3783-3793, 2011. https://doi.org/10.1158/1078-0432.ccr-10-0498
[3] L. Swystun and D. Lillicrap, Current understanding of inherited modifiers of fviii pharmacokinetic variation, Pharmacogenomics and Personalized Medicine, vol. Volume 16, p. 239-252, 2023. https://doi.org/10.2147/pgpm.s383221
[4] R. Harris, C. Berg, & D. Bowen, asgr1andasgr2, the genes that encode the asialoglycoprotein receptor (ashwell receptor), are expressed in peripheral blood monocytes and show interindividual differences in transcript profile, Molecular Biology International, vol. 2012, p. 1-10, 2012. https://doi.org/10.1155/2012/283974
[5] T. Kaden, K. Graf, K. Rennert, R. Li, A. Mosig, & M. Raasch, Evaluation of drug-induced liver toxicity of trovafloxacin and levofloxacin in a human microphysiological liver model, Scientific Reports, vol. 13, no. 1, 2023. https://doi.org/10.1038/s41598-023-40004-z
[6] B. Shu, M. Abrams, & L. Sepp‐Lorenzino, Expression of asialoglycoprotein receptor 1 in human hepatocellular carcinoma, Journal of Histochemistry & Cytochemistry, vol. 61, no. 12, p. 901-909, 2013. https://doi.org/10.1369/0022155413503662
[7] M. Hamaoka, T. Kobayashi, Y. Tanaka, H. Mashima, & H. Ohdan, Clinical significance of glypican-3-positive circulating tumor cells of hepatocellular carcinoma patients: a prospective study, Plos One, vol. 14, no. 5, p. e0217586, 2019. https://doi.org/10.1371/journal.pone.0217586
[8] J. Yang, X. Bo, X. Ding, J. Dai, M. Zhang, X. Wang, et al. Antisense oligonucleotides targeted against asialoglycoprotein receptor 1 block human hepatitis b virus replication, Journal of Viral Hepatitis, vol. 13, no. 3, p. 158-165, 2005. https://doi.org/10.1111/j.1365-2893.2005.00666.x
[9] J. Yang, X. Bo, J. Yao, N. Yang, & S. Wang, Differentially expressed cellular genes following hbv: potential targets of anti‐hbv drugs?, Journal of Viral Hepatitis, vol. 12, no. 4, p. 357-363, 2005. https://doi.org/10.1111/j.1365-2893.2005.00611.x

Customer Reviews and Q&A

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■ Q&A

Would you tell us if CSB-RP174694h contains materials of human or monkey origin?
Would you also tell us If the materials are derived from human or monkey cell lines (such as HEK293)?

Thanks for your inquiry.
1. This protein does not contain any material of human or monkey origin.
2. This protein is expressed in E. coli expression system, not in Mammalian cell (such as HEK293).

Target Background

Function

Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

Gene References into Functions

Authors report the generation of a human embryonic stem cell line WAe001-A-6 harbouring homozygous ASGR1 mutations using CRISPR/Cas9. The mutation involves a 37bp deletion, resulting in a frame shift. PMID: 28952928
PEGylated lipoplexes were well tolerated by both HEK293 (ASGP-R-negative) and HepG2 (ASGP-R-positive) cell lines and delivered DNA to the hepatoma cell line HepG2 by ASGP-R mediation at levels three-fold greater than nonPEGylated lipoplexes. PMID: 28063265
findings show that the hepatitis E virus (HEV) ORF2 protein interacts directly with the ectodomain of both ASGR1 and ASGR2; ASGPR is involved in and facilitates HEV infection by binding to ORF2 PMID: 27155063
ASGR1 can inhibit the activity of V-ATPase by interacting with LASS2, thereby suppressing the metastatic potential of hepatoma cells. PMID: 27241665
This review will focus on the mechanisms of platelet senescence with specific emphasis on the role of post-translational modifications in platelet life-span and thrombopoietin production downstream of the hepatic Ashwell-Morrell receptor, originally termed asialoglycoprotein receptor (ASGPR). PMID: 27207430
ASGR1 haploinsufficiency was associated with reduced levels of non-HDL cholesterol and a reduced risk of coronary artery disease. PMID: 27192541
Flow cytometric assessment of ASGPR expression may be a useful predictor of liver dysfunction following major hepatectomy for HCC in Chinese patients. PMID: 25404440
Anti-ASGPR antibody could be used for specific and efficient HCC CTC enrichment, and anti-P-CK combined with anti-CPS1 antibodies is superior to identification with one antibody alone in the sensitivity for HCC CTC detection. PMID: 24763545
Endocytic AMR controls TPO expression through Janus kinase 2 (JAK2) and the acute phase response signal transducer and activator of transcription 3 (STAT3) in vivo and in vitro PMID: 25485912
Anti-ASGPR levels correlate with biochemical parameters and with the severity and manifestation autoimmune processes in patients with autoimmune hepatitis. PMID: 24933948
The examined ASGPR1 expression levels by immunohistochemistry in HCC with different grades. Guidance for a targeting delivery strategy for anti-cancer drugs to HCC is suggested in this report. PMID: 23979840
the distribution of ASGR in human testis, was investigated. PMID: 23604802
the cooperative binding mode of Ca(2+) makes it possible for ASGP-R to be more sensitive to Ca(2+) concentrations in early endosomes, and plays an important role in the efficient release of ligand from ASGP-R PMID: 22613667
Constant sH2a levels suggest constitutive secretion from hepatocytes in healthy individuals; therefore, a decrease with cirrhosis suggests a diagnostic potential. PMID: 22219600
found that the asialoglycoprotein receptor (ASGPR) is involved in hepatocyte recognition of cells predestined for killing, including activated autologous T lymphocytes PMID: 21656538
Asialoglycoprotein receptor (ASGPR) interacted specifically and directly with the preS1 domain of HBV in vivo and in vitro. PMID: 21207081
Two naturally occurring ASGPR1 splice variants are produced in human hepatocytes. PMID: 20886072
our findings suggest that both fibronectin and ASGPR mediate HBsAg binding to the cell surface, which provides further evidence for the potential roles of these two proteins in mediating HBV binding to liver cells. PMID: 20364278
The minor subunit splice variants, H2b and H2c, of the human asialoglycoprotein receptor are present with the major subunit H1 in different hetero-oligomeric receptor complexes PMID: 11943787
primary renal proximal tubular epithelial cells have a functional ASGPR, consisting of the H1 and H2 subunits, that is capable of specific ligand binding and uptake PMID: 12119473
Phosphorylation-dependent interaction with molecular chaperones PMID: 12167617
the effects of palmitoylation on ASGP-R activity and function PMID: 12359251
the spacing of a Cys residue relative to the TMD in the primary protein sequence of H1 is the major determinant for successful palmitoylation PMID: 12370180
Exposure of beta-galactose results in the rapid clearance of platelets from the circulation by asialoglycoprotein receptor-expressing liver macrophages and hepatocytes. PMID: 19520807

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Subcellular Location

[Isoform H1a]: Membrane; Single-pass type II membrane protein.; [Isoform H1b]: Secreted.

Tissue Specificity

Expressed exclusively in hepatic parenchymal cells.

Database Links

HGNC: 742

OMIM: 108360

KEGG: hsa:432

STRING: 9606.ENSP00000269299

UniGene: Hs.12056

Code	CSB-EP002207HU
Abbreviation	Recombinant Human ASGR1 protein, partial
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP174694h could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) ASGR1. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP174694h could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) ASGR1.
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Recombinant Human Asialoglycoprotein receptor 1 (ASGR1), partial

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Target Background

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