Code | CSB-E09185h |
Size | 96T,5×96T,10×96T |
Promotion | |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||||||||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||||||||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||||||||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||||||||||||
| Intra-Assay Precision | Inter-Assay Precision | ||||||||||||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 | ||||||||||
n | 20 | 20 | 20 | 20 | 20 | 20 | ||||||||||
Mean(pg/ml) | 644.672 | 654.959 | 663.899 | 676.001 | 616.120 | 630.243 | ||||||||||
SD | 0.044 | 0.045 | 0.048 | 0.048 | 0.054 | 0.057 | ||||||||||
CV(%) | 4.955 | 5.028 | 5.327 | 5.281 | 6.221 | 6.492 |
To assess the linearity of the assay, samples were spiked with high concentrations of human FABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||||||||||||||
| Sample | Serum(n=4) | ||||||||||||||||
1:1 | Average % | 85 | ||||||||||||||||
Range % | 80-90 | |||||||||||||||||
1:2 | Average % | 95 | ||||||||||||||||
Range % | 89-100 | |||||||||||||||||
1:4 | Average % | 96 | ||||||||||||||||
Range % | 91-107 | |||||||||||||||||
1:8 | Average % | 90 | ||||||||||||||||
Range % | 82-98 | |||||||||||||||||
The recovery of human FABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||||||||||||||
Sample Type | Average % Recovery | Range% | ||||||||||||||||
Serum (n=5) | 93 | 85-101 | ||||||||||||||||
EDTA plasma(n=4) | 95 | 88-99 | ||||||||||||||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||
pg/ml | OD1 | OD2 | Average | Corrected | ||||||||||||||||
5000 | 2.215 | 2.292 | 2.254 | 2.150 | ||||||||||||||||
2500 | 1.623 | 1.576 | 1.600 | 1.496 | ||||||||||||||||
1250 | 1.216 | 1.184 | 1.200 | 1.096 | ||||||||||||||||
625 | 0.851 | 0.839 | 0.845 | 0.741 | ||||||||||||||||
312 | 0.518 | 0.504 | 0.511 | 0.407 | ||||||||||||||||
156 | 0.291 | 0.279 | 0.285 | 0.181 | ||||||||||||||||
78 | 0.127 | 0.119 | 0.123 | 0.019 | ||||||||||||||||
0 | 0.108 | 0.100 | 0.104 |
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The product CSB-E09185h is a sandwich ELISA kit developed to measure levels of human h-FABP in multiple samples, including serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of h-FABP in samples. The color intensity is measured at 450 nm via a microplate reader.
h-FABP, also called FABP3, is abundantly expressed in the heart and brain. It binds to long-chain fatty acids and then transports them to specific cell organelles for lipid storage and metabolism. FABP3 is considered to serve as a lipid “chaperone” regulating solubility, mobility, and utilization of fatty acids. In addition to participating in normal lipid metabolism, FABP3 is also involved in maintaining an energy supply to the heart and other body parts, improving insulin sensitivity, signaling transduction, oxidation, and transcription regulation. Overexpression of FABP3 can negatively regulate the cell cycle and inhibit the proliferation of embryonic myocardial cells and mesenchymal stem cells, leading to the development of congenital heart disease.
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