Human heparin cofactor Ⅱ,HCⅡELISA Kit

Code CSB-E09492h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
serpin peptidase inhibitor, clade D (heparin cofactor), member 1
Alternative Names
D22S673 ELISA Kit; HC II ELISA Kit; HC-II ELISA Kit; HC2 ELISA Kit; HCF2 ELISA Kit; HEP2_HUMAN ELISA Kit; Heparin cofactor 2 ELISA Kit; Heparin cofactor II ELISA Kit; HLS2 ELISA Kit; Leuserpin 2 ELISA Kit; LS2 ELISA Kit; Protease inhibitor leuserpin 2 ELISA Kit; Protease inhibitor leuserpin-2 ELISA Kit; serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1 ELISA Kit; Serpin D1 ELISA Kit; serpin peptidase inhibitor, clade D (heparin cofactor), member 1 ELISA Kit; SERPIND1 ELISA Kit; THPH10 ELISA Kit
Uniprot No.
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.25 pg/mL-80 pg/mL
0.312 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human HCⅡ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:100Average %92
Range %86-98
1:200Average %95
Range %91-100
1:400Average %98
Range %90-105
1:800Average %95
Range %89-100
The recovery of human HCⅡspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9692-102
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
802.718 2.785 2.752 2.668
402.239 2.143 2.191 2.107
201.361 1.387 1.374 1.290
100.758 0.742 0.750 0.666
50.452 0.418 0.435 0.351
2.50.227 0.219 0.223 0.139
1.250.184 0.188 0.186 0.102
00.085 0.083 0.084  
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Human SERPIND1 ELISA Kit was designed for the quantitative measurement of Human SERPIND1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.25 pg/mL-80 pg/mL and the sensitivity is 0.312 pg/mL.

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Target Background

(From Uniprot)
Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.; Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils.
Gene References into Functions
  1. Loss of Heparin Cofactor is associated with insulin resistance . PMID: 28502917
  2. The plasma level of alpha2M was found positively correlated, while HCII level was negatively correlated with higher vulnerability of carotid plaques. Both proteins were efficient in differentiating stable and vulnerable carotid plaques. These provide potential new targets for the research of carotid plaque vulnerability. Plasma alpha2M and HCII may be potential biomarkers for evaluation of the vulnerability of carotid ... PMID: 28087279
  3. We found that SERPIND1 may act as a potential oncogene in the development of ovarian cancer PMID: 27430660
  4. Molecular modelling of HCII 1-75 predicted those portions of the acidic extension that had been previously visualized in crystal structures, and suggested that an alpha-helix found between residues 26 and 36 stabilizes one found between residues 61-67. PMID: 23496873
  5. heparin co-factor II is a novel metastasis enhancer and may be used as a prognostic predictor for heparin treatment in non-small cell lung cancer PMID: 25130770
  6. In this study, we demonstrate a previously unknown role for HCII in host defense. PMID: 23656734
  7. HCII potentiates the activation of vascular endothelial cells and the promotion of angiogenesis in response to hindlimb ischemia via an AMPK-eNOS signaling pathway. PMID: 22904320
  8. Plasma heparin cofactor II activity was independently and inversely associated with the development of cardiac remodeling, including cardiac concentric change, left atrial enlargement and left ventricular diastolic dysfunction. PMID: 21107326
  9. [REVIEW]HCII protects against vascular remodeling, including atherosclerosis PMID: 20671370
  10. potential for oversulfated disaccharides in controlling HCII-regulated thrombin generation PMID: 20053992
  11. Aspartic acid residues 72 and 75 and tyrosine-sulfate 73 of heparin cofactor II promote PMID: 11856753
  12. role of basic amino acids of the A helix of heparin cofactor II in heparin- or dermatan sulfate-mediated thrombin inhibition. PMID: 12095635
  13. Altering heparin cofactor II at VAL439 (P6) either impairs inhibition of thrombin or confers elastase resistance. PMID: 12152684
  14. The interactions of HCII with various heparins in solution competition experiments using SPR show that while calcium enhances this interaction, the activity of heparin-HCII complex against thrombin is not calcium dependent but can be enhanced by it. PMID: 15247982
  15. An asymptomatic Glu428Lys mutation affecting a conserved glutamate at the hinge (P17) of the reactive loop confirms the key structural role of the P17 glutamate in serpins. PMID: 15337701
  16. after receiving stent for peripheral artery disease, patients with a high HCII activity had a 0.39-fold reduced risk for instent restenosis PMID: 15543340
  17. HCII activity increases during pregnancy, and HCII levels are significantly decreased in women with severe pre-eclampsia. PMID: 16339402
  18. Plasma heparin cofactor II activity is an independent predictor of future cardiovascular events in patients after acute myocardial infarction. PMID: 18971786
  19. These results suggest that HCII is in a position to inhibit thrombin in atherosclerotic lesions where thrombin can exert a proatherogenic inflammatory response. PMID: 19747479

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Involvement in disease
Thrombophilia due to heparin cofactor 2 deficiency (THPH10)
Protein Families
Serpin family
Tissue Specificity
Expressed predominantly in liver. Also present in plasma.
Database Links

HGNC: 4838

OMIM: 142360

KEGG: hsa:3053

STRING: 9606.ENSP00000215727

UniGene: Hs.474270

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