Product Details

Uniprot No.

Target Names

SERPIND1

Alternative Names

D22S673 antibody; HC II antibody; HC-II antibody; HC2 antibody; HCF2 antibody; HEP2_HUMAN antibody; Heparin cofactor 2 antibody; Heparin cofactor II antibody; HLS2 antibody; Leuserpin 2 antibody; LS2 antibody; Protease inhibitor leuserpin 2 antibody; Protease inhibitor leuserpin-2 antibody; serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1 antibody; Serpin D1 antibody; serpin peptidase inhibitor, clade D (heparin cofactor), member 1 antibody; SERPIND1 antibody; THPH10 antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthesized peptide derived from the N-terminal region of Human HC-II.

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

WB, IHC, IF, ELISA

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:100-1:300
IF	1:200-1:1000
ELISA	1:5000

Protocols

Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Related Products

Target Background

Function

Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.; Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils.

Gene References into Functions

Loss of Heparin Cofactor is associated with insulin resistance . PMID: 28502917
The plasma level of alpha2M was found positively correlated, while HCII level was negatively correlated with higher vulnerability of carotid plaques. Both proteins were efficient in differentiating stable and vulnerable carotid plaques. These provide potential new targets for the research of carotid plaque vulnerability. Plasma alpha2M and HCII may be potential biomarkers for evaluation of the vulnerability of carotid ... PMID: 28087279
We found that SERPIND1 may act as a potential oncogene in the development of ovarian cancer PMID: 27430660
Molecular modelling of HCII 1-75 predicted those portions of the acidic extension that had been previously visualized in crystal structures, and suggested that an alpha-helix found between residues 26 and 36 stabilizes one found between residues 61-67. PMID: 23496873
heparin co-factor II is a novel metastasis enhancer and may be used as a prognostic predictor for heparin treatment in non-small cell lung cancer PMID: 25130770
In this study, we demonstrate a previously unknown role for HCII in host defense. PMID: 23656734
HCII potentiates the activation of vascular endothelial cells and the promotion of angiogenesis in response to hindlimb ischemia via an AMPK-eNOS signaling pathway. PMID: 22904320
Plasma heparin cofactor II activity was independently and inversely associated with the development of cardiac remodeling, including cardiac concentric change, left atrial enlargement and left ventricular diastolic dysfunction. PMID: 21107326
[REVIEW]HCII protects against vascular remodeling, including atherosclerosis PMID: 20671370
potential for oversulfated disaccharides in controlling HCII-regulated thrombin generation PMID: 20053992
Aspartic acid residues 72 and 75 and tyrosine-sulfate 73 of heparin cofactor II promote PMID: 11856753
role of basic amino acids of the A helix of heparin cofactor II in heparin- or dermatan sulfate-mediated thrombin inhibition. PMID: 12095635
Altering heparin cofactor II at VAL439 (P6) either impairs inhibition of thrombin or confers elastase resistance. PMID: 12152684
The interactions of HCII with various heparins in solution competition experiments using SPR show that while calcium enhances this interaction, the activity of heparin-HCII complex against thrombin is not calcium dependent but can be enhanced by it. PMID: 15247982
An asymptomatic Glu428Lys mutation affecting a conserved glutamate at the hinge (P17) of the reactive loop confirms the key structural role of the P17 glutamate in serpins. PMID: 15337701
after receiving stent for peripheral artery disease, patients with a high HCII activity had a 0.39-fold reduced risk for instent restenosis PMID: 15543340
HCII activity increases during pregnancy, and HCII levels are significantly decreased in women with severe pre-eclampsia. PMID: 16339402
Plasma heparin cofactor II activity is an independent predictor of future cardiovascular events in patients after acute myocardial infarction. PMID: 18971786
These results suggest that HCII is in a position to inhibit thrombin in atherosclerotic lesions where thrombin can exert a proatherogenic inflammatory response. PMID: 19747479