Human microalbunminuria(MAU/ALB) ELISA kit

Instructions
Code CSB-E08970h
Size 96T,5×96T,10×96T
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Product Details

Target Name microalbunminuria(MAU/ALB)
Alternative Names alb ELISA Kit; ALBU_HUMAN ELISA Kit; Albumin (32 AA) ELISA Kit; Albumin (AA 34) ELISA Kit; cell growth inhibiting protein 42 ELISA Kit; DKFZp779N1935 ELISA Kit; GIG20 ELISA Kit; GIG42 ELISA Kit; Growth inhibiting protein 20 ELISA Kit; growth-inhibiting protein 20 ELISA Kit; OTTHUMP00000220436 ELISA Kit; OTTHUMP00000220438 ELISA Kit; OTTHUMP00000220439 ELISA Kit; PRO0883 ELISA Kit; PRO0903 ELISA Kit; PRO1341 ELISA Kit; PRO1708 ELISA Kit; PRO2044 ELISA Kit; PRO2619 ELISA Kit; PRO2675 ELISA Kit; Serum albumin ELISA Kit; UNQ696/PRO1341 ELISA Kit
Abbreviation MAU/ALB
Uniprot No. P02768
Species Homo sapiens (Human)
Sample Types serum, plasma, urine
Detection Range 0.078 μg/mL-5 μg/mL
Sensitivity 0.019 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MAU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:10000 Average % 90  
Range % 83-103  
1:20000 Average % 92  
Range % 89-97  
1:40000 Average % 96  
Range % 89-105  
1:80000 Average % 95  
Range % 86-104  
Recovery
The recovery of human MAU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 89-108  
EDTA plasma (n=4) 94 86-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
5 0.155 0.167 0.161    
2.5 0.304 0.299 0.302    
1.25 0.448 0.459 0.454    
0.625 0.730 0.794 0.762    
0.312 1.212 1.228 1.220    
0.156 1.733 1.701 1.717    
0.078 1.993 2.006 2.000    
0 2.501 2.529 2.515    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Q&A and Customer Reviews

 Q&A
Q:

Can it detect albumin?

A:
Thanks for your inquiry. Yes it can detect albumin.
Q:

We currently use your HSA ELISA kit and I was wondering if you could advise as to whether any of the constituent reagents contain Sodium Azide (NAN3).

A:
Thanks for your inquiry.
There is no constituent reagents containing Sodium Azide (NAN3) in our kit.
 Customer Reviews

Target Data

Function Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
Gene References into Functions
  1. relatively low serum concentrations of albumin may be an independent risk factor for Mild cognitive impairment in elderly. PMID: 30249938
  2. Albumin-globulin ratio in combination with CEA provides a more accurate indicator of poor prognosis and early recurrence in rectal carcinoma patients treated with preoperative chemoradiotherapy. PMID: 29996133
  3. Patients with steroid-refractory GI Acute graft versus host disease had higher fecal calprotectin levels and lower albumin levels than patients with steroid-responsive disease. PMID: 29397331
  4. Lower serum albumin concentrations in patients with pulmonary arterial hypertension are associated with higher mortality and can serve as a marker of disease severity in this patient population. PMID: 29604093
  5. This meta-analysis suggests that C-reactive protein/albumin ratio (CAR)may be a potential prognostic marker in solid cancers PMID: 28128229
  6. Thiol/Disuldife homeostasis balance and ischemia-modified albumin levels may be used a biomarker to monitor the tissue ischemia in diabetic mellitus and to discriminate the different stages of diabetic retinopathy. PMID: 29298534
  7. mitochondrial dysfunction activates the PINK1/Parkin signaling and mitophagy in renal tubular epithelial cells under albumin overload condition. PMID: 29494565
  8. Serum ischemia-modified albumin (IMA) in addition to the prevalence of small for gestational age (SGA) were significantly increased in the pre-eclampsia group. Cord blood IMA, therefore, might be a predictive biomarker for SGA in pre-eclampsia pregnancies. PMID: 29570915
  9. By using the fluorescence activatable nanosensor as the structural indicator, the stepwise conformational transitions of HSA during denaturing and the partial refolding of subdomain IIA of HSA were facilely visualized. PMID: 27232658
  10. Serum albumin adduct features were both positively and negatively associated with smoking, indicating that some adducts arise from reactions between Cys34 and constituents of cigarette smoke (e.g., ethylene oxide and acrylonitrile) while others (Cys34 oxidation products and disulfides) appear to reflect alterations in the serum redox state that resulted in reduced adduct levels in smokers. PMID: 27684351
  11. the increased lipophilicity of sulphur-containing compounds might be a reason for their better binding to HSA. PMID: 29128746
  12. hese obtained results provide an in-depth understanding of the interaction of the acid azo dye AO10 with serum albumins. PMID: 29126006
  13. A higher preoperative serum ALB level is associated with better long-term survival in intrahepatic cholangiocarcinoma patients. PMID: 29672305
  14. the presence of hydroxyl groups is capable of modulating the interaction between riparins and HSA. PMID: 28915468
  15. Spectroscopic and molecular docking studies on the interaction of human serum albumin with copper(II) complexes. PMID: 27792986
  16. aberrant HSA may play a role in the initiation/progression of type 1 diabetes mellitus. PMID: 29051099
  17. Results show that albumin from chronic hemodialyzed (CH) patients is highly modified in vivo and is not vulnerable to oxidation in the same way as normal albumin. PMID: 29554100
  18. These results indicate that low serum albumin levels correlate with increased parameter measures of hepatocellular carcinoma (HCC) aggressiveness, in addition to their role as a monitor of systemic inflammation. Decreased serum albumin might have a role in HCC aggressiveness. PMID: 28862714
  19. The hypothesis put forward that human serum albumin is a modulator and transporter of cannabinoid drugs. PMID: 28976704
  20. CRP/albumin ratio is a novel but promising, easy-to-measure, repeatable, non-invasive inflammation-based prognostic score in acute pancreatitis. PMID: 28823374
  21. homodimerization is a novel described structural alteration of the HA molecule in decompensated cirrhosis and contributes to the progressive reduction of the monomeric native HA, the only isoform provided of structural and functional integrity PMID: 27782157
  22. Low serum albumin concentration worsens prognosis of patients with stable coronary heart disease. PMID: 28413113
  23. Human serum albumin binds to Cu(I) at pH 7.4 with an apparent conditional affinity of KCu(I):serum albumin = 10(14.0) using digonal coordination in a structure that is similar to the bis-His coordination modes characterized for amyloid beta (Abeta) and the prion protein. PMID: 29166002
  24. C-reactive protein/albumin ratio (CAR), an inflammation-based prognostic score, is a potential independent predictor for disease progression and death in patients with operable non-small cell lung cancer. PMID: 27823974
  25. these peptides are identified as fragments of albumin. The isolated fragments activate MRGPRX2 and degranulate MRGPRX2 expressing LAD 2 cells in dose-dependent manner. The isolated basic peptides generated from human albumin are able to degranulate mast cells via the MRGPRX2. PMID: 28844982
  26. use of EPI-X4, a fragment of serum albumin, as a urinary biomarker for late acute GvHD was shown to be not meaningful in case control studies PMID: 27042833
  27. serum ALB level may be more useful than the degree of fibrosis for the prediction of hepatocellular carcinoma after sustained virological responses in chronic hepatitis C. PMID: 29040987
  28. Increased Ischemia-modified albumin levels and Carotid intima-media thickness may be related to increased oxidative stress and risk of atherosclerosis in individuals with Obstructive sleep apnea. PMID: 27029471
  29. Peak unbound bilirubin was primarily associated with a decrease in bilirubin albumin binding affinity in infants PMID: 29132818
  30. This meta-analysis showing increased ischaemia-modified albumin (IMA) level in both hypothyroidism and hyperthyroidism patients and its association with thyroid profile suggests that serum IMA could be used as a simple measure of increased oxidative stress in thyroid dysfunction. PMID: 27920278
  31. The optimal cut-off value was identified to be 45.6 g/L, 26.9 g/L and 1.30 for serum albumin (ALB), serum globulin (GLB) and albumin/globulin ratio (AGR) in terms of survival, respectively. PMID: 27105522
  32. Physiological serum copper concentrations found in malignancies cause unfolding induced aggregation of human serum albumin in vitro. PMID: 29122590
  33. the structural homology between HSA and ESA is big, their crystal complexes with Myr-2S-cPA demonstrate significantly different mode of binding of this LPL analogue. HSA binds three molecules of Myr-2S-cPA, whereas ESA only one. Moreover, none of the identified Myr-2S-cPA binding sites overlap in both albumins PMID: 27129297
  34. ghrelin binds to serum albumin and that this interaction impacts on the biological activity of the hormone. PMID: 27431015
  35. Higher levels of serum albumin are associated with a lower mortality rate in patients undergoing maintenance hemodialysis. PMID: 27542730
  36. results indicate that in addition to its role as a monitor of systemic inflammation, albumin may have a direct role in hepatocellular carcinoma growth inhibition, either through modulation of alpha-fetoprotein or through its actions on growth-controlling kinases PMID: 28486226
  37. These results provide an unprecedented view of how albumin interacts with Abeta and illustrate the potential of dark-state exchange saturation transfer NMR in mapping the interactions between amyloid-inhibitory proteins and amyloidogenic peptides. PMID: 28798235
  38. C-reactive protein-to-albumin ratio is a promising predictor of survival and recurrence in patients with rectal cancer treated by chemoradiotherapy followed by surgery. PMID: 28982904
  39. Lower serum albumin is associated with venous thromboembolic events in nephrotic syndrome. PMID: 28391310
  40. Data suggest that increased level of anti-Amadori-albumin autoantibodies may be used as a biomarker in disease diagnosis and its progression. PMID: 28192530
  41. Data indicate that repeated measurement after five hours showed a statistical significant difference for the fractional synthesis rate of fibrinogen but not for albumin. PMID: 28350862
  42. Data suggest that, unlike albumin with low FcRn-binding affinity, albumin with high FcRn-binding affinity (due to genetic variation/genetic engineering) is directed less to lysosomes and more to endosomes, suggestive of FcRn-directed albumin salvage from lysosomal degradation. (FcRn = neonatal Fc receptor) PMID: 28637874
  43. The mean albumin level for patients who developed heparin-induced thrombocytopenia-thrombosis (HITT) was significantly lower than that for patients who developed heparin-induced thrombocytopenia (HIT). PMID: 28289828
  44. Data suggest that posttranslational modifications of human serum albumin (HSA) typical of hyperhomocysteinemia (N and Shomocysteinylation) reduce binding affinity of catechins to HAS; the catechins used here were dietary flavonoids, epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECG), and epigallocatechin gallate (EGCG). PMID: 26658763
  45. The studies suggest that impaired endocytosis of megalin/cubilin ligands, hemoglobin and albumin, rather than heme toxicity, may be the cause of tubular proteinuria in sickle cell disease patients. PMID: 28356267
  46. Decreased serum albumin on admission is significantly associated with long-term all-cause mortality in hospital survivors of acute myocardial infarction. PMID: 27257851
  47. Intensity of systemic inflammation was assessed at all time points with C-reactive protein and albumin as prognostic covariates for overall survival in an extended Cox regression model.time-dependent effects are a prominent feature of intensity of systemic inflammation, a powerful prognostic biomarker for metastatic renal cell carcinoma. PMID: 28618965
  48. The presence of serum albumin in human eye tissues suggests its involvement in the metabolism, maintenance of tissue volume, and antioxidant reactions. PMID: 28361409
  49. study to dertermine ischemia modified albumin (IMA) level in inflammatory bowel diseases (IBD) and IBD subgroups, and to examine its relation with disease activity index; IMA levels in IBD were higher in comparison with the control group; IMA in IBD might be related with the pathogenesis of disease and correlated with the severity of the disease PMID: 26642816
  50. Serum ischemia-modified albumin was higher in type 2 DM patients than in control subjects and was higher in diabetic patients with diabetic peripheral neuropathy than in those without neuropathy. PMID: 27965983
  51. Data suggest that, in women with postmenopausal osteoporosis, serum prealbumin levels drop gradually as bone mineral density decreases. PMID: 27727007
  52. The interactions of phloretin to human serum albumin have been investigated. PMID: 26971858
  53. Vitamin D metabolites could reduce glycation modification, albeit only to a small extent. Interaction studies reveal that Vitamin D interaction with HSA can prevent protein glycation. PMID: 27364912
  54. Serum concentrations of albumin were significantly lower in patients with chronic kidney disease (CKD) stage III those with glomerular hyperfiltration. PMID: 26615054
  55. Low serum albumin and high hsCRP and vWF levels, and their correlations with cIMT, indicate that patients could be at risk of developing malnutrition-inflammation-atherosclerosis syndrome PMID: 27619398
  56. serum albumin may have a role in infection in late preterm infants PMID: 26747243
  57. Ischemia-modified albumin was statistically significantly higher in patients with nasal polyps than in controls. PMID: 26795264
  58. A novel G > T transversion at nucleotide c.270+ 1, the first base of intron 3 in the human ALB gene causes analbuminaemia. PMID: 26543026
  59. Using different computational techniques, this study identified the HSA residues whose acid-base equilibria are probably coupled with the reversible neutral to base (N --> B) transition. These include not only histidines as previously proposed but also lysines and a glutamic acid side chain. PMID: 27014836
  60. Stability of a human serum albumin (HSA)-Cd (1:1) complex was studied using a hyphenated analytical approach. Addition of d,l-homocysteine (0.5 mM) to the mobile phase abstracted Cd from its binding sites on HSA. PMID: 27294530
  61. Mutation in Albumin gene is associated with Bisalbuminaemia. PMID: 26774326
  62. Serum albumin levels are useful prognostic factors, along with platelet counts, in predicting survival in elderly patients with diffuse large B cell lymphoma. PMID: 27641425
  63. The findings reveal previously unknown pro-thrombotic activities of oxidized plasma albumin via a CD36 dependent pathway in end-stage kidney disease patients. PMID: 26905525
  64. The binding information, including the binding constants, effective quenching constant and number of binding sites showed that the binding partiality of losartan to HSA was higher than to gHSA PMID: 27470663
  65. results provide evidence that human serum albumin can act as carrier protein for phenylacetic acid. PMID: 26945842
  66. Results show structural domains from HSA not only in it's purified form, but additionally unpurified and in its native environment, human blood serum. PMID: 26385339
  67. Serum hs-CRP, but not prealbumin, values at admission are predictors of short-term mortality at hospital admission in elderly multimorbid patients. PMID: 26999249
  68. Data indicate that high levels of interleukin-6 (IL-6) and interleukin-10 (IL-10) in conjunction with albumin could improve evaluating disease's severity and predicting mortality in the critically ill patients. PMID: 26974766
  69. Decreased serum albumin levels in cancer patients were significantly associated with increased risk of VTE and mortality. Serum albumin, a marker of a cancer patient's overall prognosis, could be considered for risk assessment of important clinical outcomes such as VTE and mortality. PMID: 26764252
  70. ischemia modified albumin may act as a marker of ischemia and oxidative stress in newborns delivered to pre-eclamptic mothers. PMID: 25775261
  71. Ischaemia-modified albumin may be a valuable marker in perinatal hypoxia and intrauterine growth restriction detection. PMID: 24999531
  72. This study compared glycated human serum albumin by glucose with HSA glycated by fructose. PMID: 25735846
  73. The influence of glycation process on serum albumin binding to 5-dimethylaminonaphthalene-1-sulfonamide (DNSA), 2-(p-toluidino) naphthalene-6-sulfonic acid (TNS), has been studied. PMID: 26433342
  74. this study explored the details of imidazole derivatives binding to human serum albumin. PMID: 26474241
  75. While the weakly interacting HSA NPs did not alter the aggregation kinetic parameters of alphaSN, the rate of primary nucleation increased in the presence of PEI-HSA NPs. PMID: 26549058
  76. Data suggest glycated serum albumin contributes to oxidative stress in vascular endothelium mediated by 1) NFkappaB activation and subsequent up-regulation of NADPH oxidase and 2) eNOS uncoupling to produce superoxide anions instead of nitric oxide. PMID: 26297216
  77. Data show that serum albumin (SA) level remained an independent predictor of overall survival (OS) and median leukemia-free survival (LFS). PMID: 25669924
  78. Our study is one of the first to describe how pre-operative BMI and serum albumin and post-operative changes in these variables impact clinical and perioperative outcomes PMID: 27015568
  79. To understand binding characterization of non-steroidal anti-inflammatory drugs to albumin, this study resolved the structure of diclofenac and albumin complex by X-ray crystallography. PMID: 25958880
  80. The results showed that both patients are homozygous for the deletion of a cytosine residue in exon 5, in a stretch of four cytosines starting from nucleotide position 524 and ending at position 527 (NM_000477.5(ALB):c.527delC). PMID: 27346974
  81. ischemia-modified albumin can be effective for demonstrating hypoxia in patients with acute leukemia. PMID: 26406944
  82. Data indicate that antecedent C-reactive protein (CRP) was associated with future serum albumin. PMID: 26530188
  83. Low serum albumin level is associated with reduction in the response rate to first-line therapy in Non-Small Cell Lung Cancer. PMID: 26320482
  84. In multivariable linear regression analyses, DeltaGA/HbA1c was independently associated with diabetes duration. In conclusion, this study demonstrated that serum GA levels increase relative to HbA1c levels with time. PMID: 26484352
  85. This study evaluated the relationships among copeptin, ischemia-modified albumin (IMA), and extent of myocardial injury in patients with acute carbon monoxide poisoning. PMID: 26345979
  86. Data show that patients with a c-reactive protein/albumin ratio (CRP/Alb ratio) > 0.50 had worse survival than patients with a CRP/Alb ratio PMID: 26390126
  87. CRP and Alb are both promising biomarkers in identification of non-small cell lung cancer patients with poor prognosis and form a possible target for intensifying their therapies. PMID: 26339617
  88. Data suggest that ALB exhibits homodimerization via free cysteine-34, without any potentially immunogenic cross-linkers that are usually prerequisites for homodimerization of amyloidogenic proteins. PMID: 26554815
  89. This study suggests that antecedent low levels of ALB and total protein may be a risk factor for the acquisition of Clostridium difficile infection. PMID: 24695471
  90. albumin decreases precursor and mature forms of integrin-beta1 through reactive oxygen species-reticulum stress pathway in podocytes. PMID: 25713411
  91. Elevated levels of ischemia-modified albumin indicated oxidative damage and oxidative stress in hypothyroidism. PMID: 25862649
  92. The conformational dynamics of human serum albumin (HSA) was investigated by principal component analysis (PCA) applied to three molecular dynamics trajectories of 200 ns each. PMID: 24122695
  93. Enhanced gene silencing through human serum albumin-mediated delivery of polyethylenimine-siRNA polyplexes. PMID: 25856158
  94. Data suggest that serum IMA (ischemia-modified albumin) levels in subjects with overt and subclinical hyperthyroidism are essentially the same at baseline and with antithyroid drug therapy. PMID: 25843331
  95. initial serum albumin and 24-hour daily protein excretion (24-h DPE) before treatment as prognostic factors in patients with multiple myeloma, were investigated. PMID: 26053837
  96. Plasma free fatty acid levels influence Zn(2+) -dependent histidine-rich glycoprotein-heparin interactions via an allosteric switch on serum albumin. PMID: 25353308
  97. Glycated albumin levels were positively correlated with subclinical atherosclerosis in middle-aged and elderly Chinese. PMID: 25854289
  98. The results showed that after the adsorption of albumin on the gold nanoparticle, human serum albumin was denatured and the amount of alpha-helix significantly decreased. PMID: 25409650
  99. The molecular modeling results reveal that CIT can bind with hydrophobic pocket of HSA with hydrophobic and hydrogen bond force. PMID: 25977915
  100. The ratio of delta neutrophil index to serum albumin on emergency department admission is a prognostic marker of 28-day mortality in patients with severe sepsis. PMID: 26238097

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Involvement in disease Hyperthyroxinemia, familial dysalbuminemic (FDAH); Analbuminemia (ANALBA)
Subcellular Location Secreted
Protein Families ALB/AFP/VDB family
Tissue Specificity Plasma.
Database Links

HGNC: 399

OMIM: 103600

KEGG: hsa:213

STRING: 9606.ENSP00000295897

UniGene: Hs.418167

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