Human microalbunminuria(MAU/ALB) ELISA kit

Code CSB-E08970h
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P02768
Lead Time 3-5 working days
Abbreviation MAU/ALB
Protein Biological Process 1 others
Target Name microalbunminuria(MAU/ALB)
Alias DKFZp779N1935, PRO0883, PRO0903, PRO1341, cell growth inhibiting protein 42|growth-inhibiting protein 20|serum albumin
Species Homo sapiens (Human)
Sample Types serum, plasma, urine
Detection Range 0.078 μg/mL-5 μg/mL
Sensitivity 0.019 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Target Details Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
HGNC 316
RGD 628614
MGI 2429409
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MAU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:10000 Average % 90  
Range % 83-103  
1:20000 Average % 92  
Range % 89-97  
1:40000 Average % 96  
Range % 89-105  
1:80000 Average % 95  
Range % 86-104  
Recovery
The recovery of human MAU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 89-108  
EDTA plasma (n=4) 94 86-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
5 0.155 0.167 0.161    
2.5 0.304 0.299 0.302    
1.25 0.448 0.459 0.454    
0.625 0.730 0.794 0.762    
0.312 1.212 1.228 1.220    
0.156 1.733 1.701 1.717    
0.078 1.993 2.006 2.000    
0 2.501 2.529 2.515    
Still Have Questions? Leave a Message or Start an on-line Chat
Function Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
Involvement in disease Hyperthyroxinemia, familial dysalbuminemic (FDAH); Analbuminemia (ANALBA)
Subcellular Location Secreted
Protein Families ALB/AFP/VDB family
Tissue Specificity Plasma.
Database Links

HGNC: 399

OMIM: 103600

KEGG: hsa:213

STRING: 9606.ENSP00000295897

UniGene: Hs.418167

Pathway Th17 cell differentiation
Thyroid hormone synthesis

Q&A and Customer Reviews

Q&A
Q:

Can it detect albumin?

A:
Thanks for your inquiry. Yes it can detect albumin.
Q:

We currently use your HSA ELISA kit and I was wondering if you could advise as to whether any of the constituent reagents contain Sodium Azide (NAN3).

A:
Thanks for your inquiry.
There is no constituent reagents containing Sodium Azide (NAN3) in our kit.
Customer Reviews

Related Products

ALB Antibodies

ALB Antibodies for Homo sapiens (Human)

ALB Antibodies for Human

ALB Proteins

ALB Proteins for Homo sapiens (Human)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.

Wait!

Join the 25,000 subscribers to get research hotpots, technical tips, latest information on events, sales and offers.

Sign up now!

We don't deal in spam.