Human tight junction protein 1 (ZO1) ELISA Kit

Code CSB-E13916h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name tight junction protein 1 (zona occludens 1)
Alternative Names Tight junction protein 1 ELISA Kit; Tight junction protein ZO-1 ELISA Kit; Tight junction protein ZO1 ELISA Kit; TJP1 ELISA Kit; zo-1 ELISA Kit; Zo1 ELISA Kit; ZO1_HUMAN ELISA Kit; Zona occludens 1 ELISA Kit; Zona occludens 1 protein ELISA Kit; Zona occludens protein 1 ELISA Kit; Zonula occludens 1 protein ELISA Kit; Zonula occludens protein 1 ELISA Kit
Abbreviation TJP1
Uniprot No. Q07157
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, cell lysates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Tags & Cell Markers
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human ZO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-90  
1:2 Average % 99  
Range % 91-104  
1:4 Average % 100  
Range % 92-108  
1:8 Average % 94  
Range % 86-98  
The recovery of human ZO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-97  
EDTA plasma (n=4) 97 90-99  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.484 2.467 2.476 2.329  
500 1.707 1.678 1.693 1.546  
250 1.076 1.055 1.066 0.919  
125 0.679 0.669 0.674 0.527  
62.5 0.441 0.436 0.439 0.292  
31.2 0.297 0.287 0.292 0.145  
15.6 0.222 0.209 0.216 0.069  
0 0.149 0.145 0.147    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton
Gene References into Functions
  1. Dysfunction of the miR-455-TJP1 axis is involved in bladder cancer cell growth and metastasis. PMID: 30061227
  2. The tight junction protein ZO-1 exists in stretched and folded conformations within epithelial cells, depending on actomyosin-generated force. PMID: 29199076
  3. miR103 was upregulated in CRC. Overexpression of miR103 promoted CRC cell proliferation and migration in vitro, whereas downregulation of miR103 inhibited cell proliferation and migration. ZO1 was identified as a direct target of miR103, revealing its expression to be inversely correlated with miR103 expression in CRC samples. PMID: 29115525
  4. SHANK3 expression correlated with ZO-1 and PKCepsilon in colonic tissue of patients with Crohn's disease. The expression level of SHANK3 affects ZO-1 expression and the barrier function in intestinal epithelial cells. PMID: 28906292
  5. These results indicate the varying effects of 7-oxygenated cholesterol molecules on the expression and localization of ZO-1 depending on cell types, and suggest the contribution of 7-oxygeneted cholesterol molecules to the structural alteration of tight junctions. PMID: 29428726
  6. CTR activates AKAP2-anchored cAMP-dependent protein kinase A, which then phosphorylates tight junction proteins ZO-1 and claudin 3. PMID: 28428082
  7. the Ras signaling pathway is involved in HIV-1 Tat-induced changes in ZO-1 and NEP. PMID: 28553432
  8. decreased interaction between ZO-1 and occludin might contribute to the epiphora occurred in the transplanted submandibular glands PMID: 28332063
  9. integration of claudin-2, occludin and ZO-1 is necessary for maintaining the function of the proximal tubular epithelium. PMID: 29252987
  10. Endothelial cellsTLR4 strongly regulates retinal vessel permeability by reducing expression of occludin and zonula occludens 1. PMID: 29136627
  11. The role of estrogens in the regulation of ZO-1 and estrogen receptors 1 and 2 was evaluated in human primary gut tissues by immunohistochemistry, immunofluorescence and qPCR. PMID: 28867253
  12. Aberrant expression of the tight junction molecules claudin-1 and zonula occludens-1 mediates cell growth and invasion in oral squamous cell carcinoma cells. PMID: 27436828
  13. ZO-1-occludin interactions regulate multiple phases of epithelial polarization by providing cell-intrinsic signals that are required for single lumen formation. PMID: 27802160
  14. It is postulated that ZO-1, when not phosphorylated by PKC, keeps Octn2 in an active state, while elimination of this binding in DeltaPDZ mutant or after ZO-1 phosphorylation leads to diminution of Octn2 activity. PMID: 28257821
  15. Results uncovered ZO-1 as part of a signaling node activated by VEGF, but not Ang-1, that specifically modulates endothelial cells proliferation during angiogenesis. PMID: 26846344
  16. Data suggest that long noncoding RNA PlncRNA1 and microRNA miR-34c bound together to regulate the expressions of MAZ, ZO-1 and occludin. PMID: 28153728
  17. ZO-1 highly expresses in cell-cell junctions and is related to odontoblast differentiation, which may contribute to dental pulp repair or even the formation of an odontoblast layer. PMID: 27109589
  18. this study shows that the expression and the immunoreactivity of ZO-1 is decreased in the nasal epithelium of patients with allergic rhinitis PMID: 27216347
  19. The frequency of alleles and genotypes of rs2291166 gene polymorphism TJP1 was determined in the Mexico Mestizos population. The ancestral allele was the most prevalent. The conformational effect of this amino acid change was performed in silico. PMID: 26259745
  20. We identified potential nuclear and membrane biomarkers (increased expression of ZO-1, caveolin-1 and P2X7 receptor) of risks for placenta and pregnancy PMID: 26657896
  21. OCLN and ZO1 levels appear to be early prognostic markers in patients suffering from sepsis. PMID: 26863122
  22. The data suggest that ZO-1, along with CD38 and Zap-70, plays a role in cell cycle regulation in chronic B cell leukemia, and may be used as a prognostic marker in the disease monitoring. PMID: 26306999
  23. These data provide the first evidence that beta-catenin and ZO-1 are direct targets of E7 of the oncogenic beta-human papillomavirus types 5 and 8. PMID: 26645068
  24. Report TNF-alpha/Il6 mediated dysregulation of zonula occludens-1 properties in human brain microvascular endothelium. PMID: 25953589
  25. upon specific knockdown of the accessory TJP, ZO-1, undifferentiated NSCs showed decreased levels of key stem cell markers PMID: 25892136
  26. HTT may inhibit breast tumor dissemination through maintenance of ZO1 at tight junctions. PMID: 26293574
  27. These results suggest that the localization of ZO-1 in cell-cell contacts is differently regulated by activation and inhibition of JNK and/or p38 MAPK depending on the incubation period. PMID: 25435485
  28. CFTR colocalizes with ZO-1 at the tight junctions of trachea and epididymis, and is expressed before ZO-1 in Wolffian ducts. PMID: 25107366
  29. Phosphorylation state of the tyrosine of claudin-1 and claudin-2 regulates interaction with ZO1. PMID: 26023235
  30. miR-18a and RUNX1 could reversely regulate the permeability of blood-tumor barrier as well as the expressions and distributions of ZO-1, occludin and claudin-5. PMID: 25452107
  31. Zonula occludens-1, occludin and E-cadherin expression and organization in salivary glands PMID: 25248927
  32. ZO-1 was internalized and shown to accumulate in the cytoplasm of human podocytes in an IL-13 dose-dependent manner. PMID: 25683991
  33. The decreased UCP2 expression and increased ZO-1 expression suggest that the oxidative stress-induced mitochondrial dysfunction and tight junction formation may play pivotal roles in the progress of NVG. PMID: 23835672
  34. ZO-1 is a central regulator of VE-cadherin-dependent endothelial junctions that orchestrates the spatial actomyosin organization. PMID: 25753039
  35. Tjp1 expression was decreased in glomerular diseases in human and animal models, our results indicate that the suppression of Tjp1 could directly aggravate glomerular disorders, highlights Tjp1 as a potential therapeutic target. PMID: 25184792
  36. In conclusion, our present study indicated that miR-34c regulated the permeability of BTB via MAZ-mediated expression changes of ZO-1, occludin, and claudin-5. PMID: 25201524
  37. ZO-1 showed a tendency to be detected more intensely in myocardial infarction and ischemic heart disease myocardial tissue then in asphyxiation or drowning. PMID: 24368520
  38. ZO-1 gene shows a hypermethylation status in children with NHL. PMID: 24927439
  39. gene expression is regulated by p38MAPK PMID: 23856837
  40. High expression of ZO-1 is associated with good prognosis in non-small cell lung cancer. PMID: 24294375
  41. The LIM domain protein FHL1C interacts with tight junction protein ZO-1 contributing to the epithelial-mesenchymal transition of a breast adenocarcinoma cell line. PMID: 24657059
  42. luciferase assays and chromatin immunoprecipitation assays showed that KLF4 up-regulated the promoter activities and interacted with "CACCC" DNA sequence presented in the promoters of ZO-1, occludin, and claudin-5. PMID: 24318462
  43. our results identify a novel regulatory pathway involving the interplay between ZO-1, alpha5-integrin and PKCepsilon in the late stages of mammalian cell division. PMID: 23967087
  44. Data suggest components of diet supplements (here, glutamine/arginine) can improve permeability and tight junction protein expression (TJP1/occludin) in enterocytes exposed to deleterious effects of antineoplastic agents (here, methotrexate). PMID: 23428392
  45. ZO-1 expression is correlated with malignant phenotypes of GIST. PMID: 23820955
  46. Vascular endothelial tight junctions and barrier function are disrupted by 15(S)-hydroxyeicosatetraenoic acid partly via protein kinase C epsilon-mediated zona occludens-1 phosphorylation at threonine 770/772. PMID: 24338688
  47. The presence of neural cells (PC12 cells or trigeminal neurons) markedly promoted the stratification of HCE cells as well as increased the amounts of N-cadherin mRNA and protein in these cells. PMID: 24327615
  48. The methylation positivity rates of the ID4 and ZO-1 genes in the bone marrow and paraffin-embedded lymphoma tissues of non-Hodgkin lymphoma patients were significantly higher compared to the rates in the Hodgkin lymphoma patients. PMID: 23670122
  49. Proteomic identification of ZO-1 binding partners and associated proteins that form tight junction complexes. PMID: 23553632
  50. Single nucleotide polymorphisms in TJP1 is associated with response to antipsychotic agents in schizophrenia. PMID: 23241943
  51. findings suggest that ZO-1 and alpha-catenin are important for gap junctions containing Cx43 in the LECs PMID: 23151788
  52. 25-hydroxyvitamin D3 increases the permeability of normal bronchial airway epithelial cell monolayer in vitro, but this effect is not mediated by upregulation of ZO-1 expression. PMID: 21764691
  53. Modeling the "airway reopening" process, examined consequent increases in pulmonary epithelial plasma membrane rupture, paracellular permeability, and disruption of the tight junction proteins zonula occludens-1 and claudin-4. PMID: 22898551
  54. It is concluded that attenuated expression of the TJ proteins occludin and ZO-1 in human gastric epithelial cells could be involved in clopidogrel-induced gastric mucosal injury through activation of the p38 MAPK pathway PMID: 23220562
  55. PM induces marked increases in vascular permeability via ROS-mediated calcium leakage via activated TRPM2, and via ZO-1 degradation by activated calpain. PMID: 22931549
  56. The effect of myoferlin on the expression of ZO-1 in airway epithelial cells indicates its role in membrane fusion events that regulate cell detachment and apoptosis within the airway epithelium. PMID: 22808170
  57. tight junction protein ZO-1 (zonula occludens protein 1) has recruiting/scaffolding functions in the junctional complex of epithelial and endothelial cells PMID: 22671591
  58. We define the interface between the ZO-1 PDZ3-SH3-U5-GuK (PSG) and occludin coiled-coil (CC) domains. PMID: 22711802
  59. CPEB-mediated zonal occludens-1 mRNA localization is essential for tight-junction assembly and mammary epithelial cell polarity PMID: 22334078
  60. study adds new components to the multi-dentate membrane targeting mechanism and highlights the role of N- and C-terminal PDZ extensions of PSD-95/ZO-1 in the regulation of syntenin-1 plasma membrane localization PMID: 22673509
  61. An implication of ZO-1 in CXCL8/IL-8 regulation. Because of the major implications of CXCL8/IL-8 in tumor invasion, such a regulation could play an important role in breast cancer progression. PMID: 22064657
  62. ZO-1 protein is necessary for Cx50 intercellular channel formation PMID: 21965293
  63. Results demonstrate a role for afadin in the regulation of vascular barrier function via coordination of adherens junction-tight junction and p120-catenin-ZO-1 interactions. PMID: 21732359
  64. a 184-amino acid region (from amino acids 39 to 223) at the N-terminal region of Ubn-1 was responsible for the interaction with the PDZ2 domain of ZO-1 PMID: 22245583
  65. The Src homology 3 domain is required for junctional adhesion molecule binding to the third PDZ domain of the scaffolding protein ZO-1. PMID: 22030391
  66. this study identifies a new mechanism of gap junction intercellular communication regulation in which endogenous g-protein coupled receptor agonists dynamically alter interactions between connexin43 and zonula occludens-1 PMID: 21872657
  67. A crucial role is indicated crucial for Rho signaling and CREB in modulation of nuclear localization of ZO-1 and maintaining the integrity of endothelial monolayers. PMID: 22219277
  68. Data suggest that the degradation of tight junction proteins ZO-1, claudin-5 and occludin by MMP-2 and -9 secreted by leukemic cells constitutes an important mechanism in the BBB breakdown which contributes to the invasion of leukemic cells to the CNS. PMID: 21857898
  69. The significant decline in TJP1 expression in CD patients, particularly in those with coexisting T1D, was accompanied by an increase in FOXP3 expression. PMID: 21832836
  70. The structure of the PDZ3-SH3-GuK tandem of ZO-1 protein suggests a supramodular organization of the membrane-associated guanylate kinase (MAGUK) family scaffold protein core. PMID: 21965684
  71. expression of ZO-1 and N-cadherin may reflect the mechanisms leading to rosette formation in neuroendocrine tumors PMID: 21785870
  72. ZO-1 regulates the rate of undocked connexon aggregation into gap junctions, enabling dynamic partitioning of Cx43 channel function between junctional and proximal nonjunctional domains of plasma membrane PMID: 21411628
  73. The zo-1 and id4 genes in multiple myeloma cell lines all were methylation positive, and the methylation positive rates were significantly higher than in normal bone marrow. PMID: 21129259
  74. Compared with healthy individuals, zo-1 gene in acute leukemia patients is hypermethylated and with different degrees in various phases of leukemia. PMID: 20723289
  75. In lower neovaginal mucosa where squamous metaplasia occurred, the expression of occludin and ZO-1 was significant;y elevated. PMID: 20003786
  76. Data show that the redistribution of ZO-1 is mediated by phosphorylation of ezrin-radixin-moesin (ERM) proteins. PMID: 21486949
  77. The methylation of zo-1 may be involved in the genesis of acute leukemia. PMID: 19840438
  78. ZO-1 and PLEKHA7 are paracingulin-interacting proteins that are involved in its recruitment to epithelial tight and adherens junctions, respectively PMID: 21454477
  79. Solution structure of the second PDZ domain of Zonula Occludens 1. PMID: 21387411
  80. occludin S408 dephosphorylation regulates paracellular permeability by remodeling tight junction protein dynamic behavior and intermolecular interactions between occludin, ZO-1, and select claudins PMID: 21536752
  81. ZO-1 gene methylation might be involved in the tumorigenesis of acute leukemia. PMID: 19954602
  82. There was a significant difference of ZO-1 mRNA expression levels between carcinoma group and control group. PMID: 21342646
  83. ZO-1, a classical scaffold protein with accepted roles in maintaining cell-cell adhesions in stable tissues, also has an active role in cell migration during processes such as tissue development and remodelling. PMID: 21240187
  84. These findings demonstrate that besides their known function in tight junction assembly and intercellular communication, zona occludens proteins and their binding partners may play a novel role in podosome formation and associated function. PMID: 20930113
  85. PLEKHA7 is a cytoplasmic component of the epithelial adherens junction belt, distinct from ZO-1 and E-cadherin PMID: 20808826
  86. the first PDZ domain of zona occludens-1 (ZO-1) and 2 (ZO-2) interacts with the carboxy-terminal PDZ binding motif of TAZ PMID: 20850437
  87. The effects of diesel exhaust particles on endothelial vascular cell permeability and down regulation of ZO-1 are reported. PMID: 20576493
  88. Demonstrate that a decrease in ZO-1 expression reduces human trophoblast cell-cell fusion and differentiation. PMID: 20200207
  89. These results suggested that although hypercholesterolemic serum could not change the expression of ZO-1, it could change the distribution and increase the permeability in endothelial cells through PI3K signaling pathway. PMID: 20150971
  90. Insights into regulated ligand binding sites from the structure of ZO-1 Src homology 3-guanylate kinase module. PMID: 20200156
  91. ZO-1, occludin, and E-cadherin are downregulated in carcinomas arising from various compartments of the biliary tract PMID: 19184677
  92. Study shows that ZO isoforms bind PtdInsPs and offers an alternative regulatory mechanism for the formation and stabilisation of protein complexes in the nucleus. PMID: 19784548
  93. Rab13,VASP,and ZO-1 were found in apical tight junctions in normal epithelium but were dislocated to the basolateral position in patients with inactive Crohn's disease. In patients with ulcerative colitis,these tight junction proteins were not dislocated. PMID: 19885626
  94. The ZO-1 promoter region in bone marrow of myelodysplastic syndrome patients shows a hypermethylation status, which is specific for MDS. PMID: 18315903
  95. Data show that connexin 43 (cx43) binds alpha-tubulin equally well as beta-tubulin, and that ZO-1 binds directly to Cx43. PMID: 12064592
  96. oxidative stress induces tyrosine phosphorylation and cellular redistribution of occludin-ZO-1 and E-cadherin-beta-catenin complexes by a tyrosine-kinase-dependent mechanism PMID: 12169098
  97. Tyrosine phosphorylation of ZO-1 leads to down-regulation of the function of ZO-1 and dedifferentiation of the glands in colorectal cancers. These phenomena contribute to liver metastases, & redifferentiation of the glands occurs in the liver metastases. PMID: 12708492
  98. dedifferentiation and a decreased expression of E-cadherin and ZO-1 are closely related to liver metastasis PMID: 12725331
  99. Modulatory role of VIPergic submucosal neuronal pathways on intestinal epithelial barrier permeability and ZO-1 expression. PMID: 12881224
  100. Interactions with ZO-1 and ZO-2, in particular, may mediate recruitment of armadillo repeat gene deletes in velocardiofacial syndrome protein to the plasma membrane and the nucleus PMID: 15456900

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Subcellular Location Cell membrane, Peripheral membrane protein, Cytoplasmic side, Cell junction, tight junction, Cell junction, Cell junction, gap junction, Cell projection, podosome
Protein Families MAGUK family
Tissue Specificity The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
Database Links

HGNC: 11827

OMIM: 601009

KEGG: hsa:7082

STRING: 9606.ENSP00000281537

UniGene: Hs.743990

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