Mouse Aspartate Aminotransferase (AST) ELISA kit

Product Details

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Target Background

Product Details

Target Name

glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)

Alternative Names

Got2 ELISA kit; Got-2Aspartate aminotransferase ELISA kit; mitochondrial ELISA kit; mAspAT ELISA kit; EC 2.6.1.1 ELISA kit; EC 2.6.1.7 ELISA kit; Fatty acid-binding protein ELISA kit; FABP-1 ELISA kit; Glutamate oxaloacetate transaminase 2 ELISA kit; Kynurenine aminotransferase 4 ELISA kit; Kynurenine aminotransferase IV ELISA kit; Kynurenine--oxoglutarate transaminase 4 ELISA kit; Kynurenine--oxoglutarate transaminase IV ELISA kit; Plasma membrane-associated fatty acid-binding protein ELISA kit; FABPpm ELISA kit; Transaminase A ELISA kit

Abbreviation

GOT2

Uniprot No.

P05202

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, cell culture supernates

Detection Range

1.56 mIU/mL-100 mIU/mL

Sensitivity

0.39 mIU/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse AST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-95
1:2	Average %	93
	Range %	87-98
1:4	Average %	91
	Range %	89-94
1:8	Average %	94
	Range %	90-98

Recovery

The recovery of mouse AST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-99
EDTA plasma (n=4)	98	94-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
100	2.915	2.898	2.907	2.720
50	2.475	2.432	2.454	2.267
25	2.015	2.002	2.009	1.822
12.5	1.376	1.342	1.359	1.172
6.25	0.731	0.717	0.724	0.537
3.12	0.472	0.445	0.459	0.272
1.56	0.312	0.301	0.307	0.120
0	0.189	0.185	0.187

Materials provided

A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Mouse AST antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled AST antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

Materials not provided

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse GOT2 ELISA Kit was designed for the quantitative measurement of Mouse GOT2 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 1.56 mIU/mL-100 mIU/mL and the sensitivity is 0.39 mIU/mL.

Target Background

Function
(From Uniprot)

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.

Gene References into Functions

GOT2-mediated ATP synthesis may be a pivotal mechanism underlying the protective effect of febuxostat against neurodegeneration in EA PMID: 29107957
it is quite likely that the down regulation of GOT2 after blast exposure is playing a significant role in mitochondrial dysfunction after blast exposure PMID: 23600763
The role of mAspAT in vivo is to control the levels of endogenous kynurenic acid (KYNA) through modulation of the mAspAt in the mouse brain. PMID: 20977429
KAT IV inhibited by equimolar tryptophan and aspartate, and half-life is 6.5 minutes at 65 degree C. PMID: 20482848

Subcellular Location

Mitochondrion matrix. Cell membrane.

Protein Families

Class-I pyridoxal-phosphate-dependent aminotransferase family

Tissue Specificity

Detected in brain (at protein level).

Database Links

KEGG: mmu:14719

STRING: 10090.ENSMUSP00000034097

UniGene: Mm.230169

Code	CSB-E12649m
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.