Mouse Bone-specific alkaline phosphatase (BALP) ELISA kit

Instructions
Code CSB-E11914m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Bone-specific alkaline phosphatase
Alternative Names Alpl ELISA Kit; Akp-2 ELISA Kit; Akp2 ELISA Kit; Alkaline phosphatase ELISA Kit; tissue-nonspecific isozyme ELISA Kit; AP-TNAP ELISA Kit; TNSALP ELISA Kit; EC 3.1.3.1 ELISA Kit; Alkaline phosphatase 2 ELISA Kit; Alkaline phosphatase liver/bone/kidney isozyme ELISA Kit
Abbreviation BALP
Uniprot No. P09242
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.25 ng/mL-80 ng/mL
Sensitivity 0.31 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse BALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 99  
Range % 94-104  
1:10 Average % 92  
Range % 88-98  
1:20 Average % 84  
Range % 80-88  
1:40 Average % 97  
Range % 92-102  
Recovery
The recovery of mouse BALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 92-98  
EDTA plasma (n=4) 92 87-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
80 2.912 2.811 2.862 2.707  
40 2.321 2.203 2.262 2.107  
20 1.468 1.447 1.458 1.303  
10 0.877 0.866 0.872 0.717  
5 0.522 0.534 0.528 0.373  
2.5 0.350 0.362 0.356 0.201  
1.25 0.259 0.267 0.263 0.108  
0 0.154 0.155 0.155    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

 Customer Reviews
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Sample type: Cell culture supernatant

Sample species: Mouse

Review: The serum samples were subjected to detection of soluble receptor activator of nuclear factor kappa-Β ligand (RANKL) and bone alkaline phosphatase (BALP; cat#:CSB-E11914m, respectively), according to the manufacturer\'s instructions. In agreement, WT mice presented significantly higher circulating BALP concentration than those ASK KO mice.

By Anonymous

Sample type: Serum

Sample species: Mouse

Review: The level of serum BALP, an indicator of osteoblast activation, was significantly decreased in the OVX group compared to the Con group, but GSGE treatment increased serum BALP level compared to the OVX group.

By Anonymous

Sample type: Serum

Sample species: Mouse

Review: We measured the serum levels of a bone formation marker, bone-specific alkaline phosphatase (BALP) using commercially available ELISA assays according to the manufacturers’ instructions. We did not detect a notable difference in the BALP serum levels.

By Anonymous

Target Background

Function
(From Uniprot)
This isozyme may play a role in skeletal mineralization.
Gene References into Functions
  1. intestinal alkaline phosphatase knockout mice display higher intestinal Ca uptake, which over time appears to correlate with a positive effect on the biomechanical properties of trabecular bone. PMID: 29234952
  2. Study results from cerulein induced pancreatitis model in TNAP+/- mice show that altered TNAP expression results in heightened pancreatic inflammation, which may be explained by an augmented response of neutrophils and by a higher sensitivity of acinar cells to cerulein injury. PMID: 30251694
  3. TNAP activation in vascular smooth muscle cells (VSMCs) appears sufficient to induce calcification. TNAP activation in VSMCs stimulates expression of chondrocyte markers. PMID: 27932058
  4. Our results offer clear evidence that TNAP modulates T lymphocyte function and specifically T cell-dependent colitis. PMID: 28039309
  5. The results are the first to demonstrate a role for ENC1 in the control of osteoblast differentiation. Additionally, the contrasting mineralization phenotypes and transcriptional patterns seen with coordinate knockdown of both ENC1 isoforms vs selective knockdown of 67 kDa ENC1 suggest opposing roles for the isoforms in regulation of osteoblastic differentiation, through effects on Alpl expression and phosphate cellular PMID: 27996212
  6. TNAP overexpression in vascular endothelium in mice leads to an unusual course of coronary atherosclerosis and was accompanied by the reduction in body weight and left ventricular ejection fraction. PMID: 29023576
  7. Data, including data from studies using cells from transgenic/knockout mice, suggest that Med1 plays role in enamel formation; Med1 induces Alpl via stimulation of Notch1 signaling by forming Notch1-RBP-Jk complex on Alpl promoter. (Med1 = mediator complex subunit 1; Alpl = alkaline phosphatase, liver-bone-kidney; Notch1 = Notch gene homolog 1; RBP-Jk = kappa J region recombining binding protein suppressor of hairless) PMID: 28673966
  8. These analyses revealed that TNAP deficient mice present an increased proliferation of neural precursors, an altered neuronal morphology, and an augmented neuronal activity. We found that these alterations were associated with a partial downregulation of the purinergic P2X7 receptor (P2X7R). PMID: 27466191
  9. Despite similar deficiencies in alkaline phosphatase, Alpl(-/-) mice develop craniosynostosis and a brachycephalic/acrocephalic craniofacial shape of variable penetrance. PMID: 26605996
  10. Prevention of lethal murine hypophosphatasia by neonatal ex vivo gene therapy using lentivirally transduced bone marrow cells expressing Akp-2. PMID: 26467745
  11. TNAP in the vasculature contributes to the pathology of medial vascular calcification and that it is a druggable target. PMID: 25428889
  12. In cardiac fibroblasts, TNAP expression and activity is induced by sFRP2. PMID: 25972450
  13. p107 is required for the efficient recruitment of an activating SWI/SNF chromatin-remodeling complex, an essential event in Alpl induction. PMID: 25182511
  14. Inhibition of rhBMP-2-induced ALP activity by intracellular delivery of SMURF1 in murine calvarial preosteoblast cells. PMID: 24288199
  15. Findings demonstrate that Alpl(-/-) mice exhibit a craniofacial skeletal phenotype similar to that seen in infants with HPP, including true bony craniosynostosis in the context of severely diminished bone mineralization PMID: 25014884
  16. CD73 and TNAP play interactive roles to metabolize luminally applied 5'-AMP in the renal vasculature such that inhibition of both is required to inhibit the production of adenosine. PMID: 24990899
  17. TNAP plays a role in governing the phosphorylation status of phospholamban in the sarcoplasmic reticulum. PMID: 25015959
  18. Taken together, these data indicate that ATF3 is a novel negative regulator of osteoblast differentiation by specifically suppressing ALP gene expression in preosteoblasts. PMID: 24315873
  19. data suggest that the promineralization role of TNAP may be related not only to its accepted pyrophosphatase activity but also to its ability to modify the phosphorylation status of OPN. PMID: 23427088
  20. mineralization abnormalities of dentin; reduced overall mineralization with decreased matrix vesicle mineralization in the Phospho1(-/-) mice; almost complete absence of matrix vesicles in mice; further reduction in mineralization. PMID: 23694930
  21. In the cerebral cortex, myelinated axons, while present in wild-type, were absent in the Akp2( -/- ) mice and these animals also displayed a significantly increased proportion of immature cortical synapses. PMID: 22696173
  22. a link between ATRA-induced mL/B/K-ALP gene transcription and chromatin remodeling PMID: 22270475
  23. Results demonstrate that TNAP, regulating both ligand availability and protein expression of P2X7 receptor, is essential for axonal development. PMID: 21289095
  24. Data show that alkaline phosphatase (AP) activity in brain vessels and parenchyma in which AP exhibits specific patterns is attributable to TNAP. PMID: 21191615
  25. We show that TNAP knockdown reduces cell proliferation and differentiation into neurons or oligodendrocytes PMID: 20849921
  26. These results demonstrate that calcium ions released from apatite are important in the synergistic effect of 20alpha-HC and apatite. PMID: 20683129
  27. Once the HT2B receptor is expressed, it constitutively controls tissue-nonspecific alkaline phosphatase activity at a post-translational level along the overall period of bone mineral deposition PMID: 20573958
  28. Tissue-nonspecific alkaline phosphatase is the enzyme that hydrolyzes both ATP and inorganic pyrophosate in matrix vesicles. PMID: 19874193
  29. correction of bone mineralization abnormalities in knockout mice null for both the TNAP (Akp2) and PC-1 (Enpp1) genes PMID: 12082181
  30. The Alpl (Akp2) gene is located within the quantitative trait locus region for alkaline phosphatase activity on chromosome 4. PMID: 16159911
  31. alkaline phosphatase 2(Hpp/Hpp) mice develop late-onset skeletal disease, notably defective endochondral ossification and bone mineralization that leads to arthropathies of knees and shoulders PMID: 17539739
  32. The process selectively internalizes IAP and may contribute to the appearance of the enzyme in serum and surfactant-like particles. PMID: 17947448
  33. Results show that inorganic phosphate (P(i)) levels and TNSALP activity increased in response to androgen/androgen receptor (AR), and P(i) signals increase the expression and translocation of AR. PMID: 18838539
  34. TNAP-AID mice expressed alpha-fetoprotein and had deleterious mutations in the tumour suppressor gene Trp53, some of which corresponded to those found in human cancer. PMID: 18997814

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Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor
Protein Families Alkaline phosphatase family
Database Links

KEGG: mmu:11647

STRING: 10090.ENSMUSP00000030551

UniGene: Mm.288186

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