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| Code | CSB-E11914m |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse BALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 99 | ||||
| Range % | 94-104 | |||||
| 1:10 | Average % | 92 | ||||
| Range % | 88-98 | |||||
| 1:20 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:40 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| The recovery of mouse BALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 92-98 | ||||
| EDTA plasma (n=4) | 92 | 87-97 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This Mouse BALP ELISA Kit was designed for the quantitative measurement of Mouse BALP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.25 ng/mL-80 ng/mL and the sensitivity is 0.31 ng/mL .
Sample type: Cell culture supernatant
Sample species: Mouse
Review: The serum samples were subjected to detection of soluble receptor activator of nuclear factor kappa-Β ligand (RANKL) and bone alkaline phosphatase (BALP; cat#:CSB-E11914m, respectively), according to the manufacturer\'s instructions. In agreement, WT mice presented significantly higher circulating BALP concentration than those ASK KO mice.
By Anonymous
Sample type: Serum
Sample species: Mouse
Review: The level of serum BALP, an indicator of osteoblast activation, was significantly decreased in the OVX group compared to the Con group, but GSGE treatment increased serum BALP level compared to the OVX group.
By Anonymous
Sample type: Serum
Sample species: Mouse
Review: We measured the serum levels of a bone formation marker, bone-specific alkaline phosphatase (BALP) using commercially available ELISA assays according to the manufacturers’ instructions. We did not detect a notable difference in the BALP serum levels.
By Anonymous
