Mouse Coagulation Factor XIII A1 Polypeptide(F13A1)ELISA kit

Code CSB-E13519m
Size 96T,5×96T,10×96T
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Product Details

Target Name
coagulation factor XIII, A1 polypeptide
Alternative Names
F13a1 ELISA kit; F13aCoagulation factor XIII A chain ELISA kit; Coagulation factor XIIIa ELISA kit; EC 2.3.2.13 ELISA kit; Protein-glutamine gamma-glutamyltransferase A chain ELISA kit; Transglutaminase A chain ELISA kit
Abbreviation
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.56 ng/mL-100 ng/mL
Sensitivity
0.39 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Blood Coagulation
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse F13A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %87
Range %82-93
1:200Average %95
Range %90-99
1:400Average %93
Range %87-98
1:800Average %102
Range %96-108
Recovery
The recovery of mouse F13A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9691-102
EDTA plasma (n=4)9893-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
1002.684 2.657 2.671 2.487
502.398 2.317 2.358 2.174
251.865 1.898 1.882 1.698
12.51.247 1.214 1.231 1.047
6.250.673 0.699 0.686 0.502
3.120.488 0.498 0.493 0.309
1.560.357 0.386 0.372 0.188
00.183 0.184 0.184  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse F13A1 ELISA Kit was designed for the quantitative measurement of Mouse F13A1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL .

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Target Background

Function
(From Uniprot)
Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
Gene References into Functions
  1. Factor XIII-A and TG2 regulate resorption, adipogenesis and plasma fibronectin homeostasis in bone and bone marrow. PMID: 28387755
  2. Suggest that resident macrophages maintain plasma FXIII-A and exclude the platelet lineage as a major contributor. PMID: 28596376
  3. These findings provide insight into assembly of the fibrinogen/FXIII-A2B2 complex in both physiologic and therapeutic situations. PMID: 27561317
  4. Mast cells have the ability to produce and degrade FXIIIA depending on their chymase expression profile: mast cells expressing chymase degrade FXIIIA, whereas mast cells that do not express chymase mainly produce FXIIIA PMID: 27302551
  5. A lack of factor XIII leads to prolonged clotting times, increased signs of inflammation, and elevated bacterial dissemination in streptococcal skin diseases. PMID: 27338836
  6. FXIII is a significant determinant of mucosal healing and clinical outcome following inflammatory colitis induced mucosal injury PMID: 26098308
  7. These findings expose a newly recognized, essential role for fibrin crosslinking during whole blood clot formation and consolidation and establish FXIIIa activity as a key determinant of thrombus composition and size. PMID: 26324704
  8. related to islet dysfunction during the development of type 2 diabetes PMID: 26018251
  9. 5-HT inhibits Factor XIII-A-mediated plasma fibronectin matrix assembly and crosslinking in osteoblast cultures via direct competition with transamidation PMID: 25460579
  10. High Transglutaminase factor XIII promotes arthritis through mechanisms linked to inflammation and bone erosion. PMID: 25336631
  11. Fibrinogen from mice harboring the Fibgamma390-396A mutation exhibited reduced binding to FXIII, and plasma from these mice exhibited delayed FXIII activation and fibrin crosslinking, indicating these residues mediate FXIII binding and activation. PMID: 24983320
  12. FXIII-A serves as a preadipocyte-bound proliferation/differentiation switch that mediates effects of hepatocyte-produced circulating pFN. PMID: 24934257
  13. Physiological significance of mouse TG2 and Factor XIII through identification of substrate proteins in MC3T3-E1 cells. PMID: 22045386
  14. Studies indicate that in FXIII-A-knockout mice, tail-tip bleeding times are doubled and clot stability, assessed by thromboelastography, was impaired compared with wild-type mice. PMID: 23075332
  15. FXIIIA mRNA was regulated by the MAP kinase pathway. PMID: 21959563
  16. Data indicate that FXIIIa-targeted probe signals showed good overlap with immune-fluorescent fibrin staining images. PMID: 22272319
  17. factor XIIIA transglutaminase activity regulates osteoblast matrix secretion and deposition by affecting microtubule dynamics PMID: 21283799
  18. Inhibition of TG2 and FXIIIA during early development of lesions reduced the macrophage content in the media of atherosclerotic vessels, while not affecting lesion size or geometric remodeling PMID: 20810110
  19. Knockout mouse studies suggest that plasma FXIII-A does not derive primarily (if at all) from circulating platelets and indeed may not derive from the megakaryocyte lineage. PMID: 20086247
  20. All FXIII2/2 and FXIII2/+ mice died within 5 days after MI from left ventricular (LV) rupture and had locally reduced levels of FXIII. PMID: 17183677
  21. Factor XIII A subunit deficieny is associated with impaired clot retraction PMID: 19996413
  22. impaired clot stabilization in FXIII-A mutant mice PMID: 12529747
  23. Maternal FXIII plays a critical role in uterine hemostasis and maintenance of the placenta during gestation. PMID: 12933578
  24. platelet FXIII and calpain have roles in regulating integrin alpha(IIb)beta3 adhesive function PMID: 15131115
  25. FXIII has an essential role in wound repair and remodeling PMID: 16113836
  26. analysis of the expression, localization and activity of TG2 and FXIIIA in mineralizing tissues [review] PMID: 16368540
  27. Results demonstrate that MC3T3-E1/C14 osteoblasts express two transglutaminase isoforms--TG2 and FXIIIA. PMID: 16469487
  28. the entire FXIIIA gene is expressed, thus indicating that the 37-kDa FXIIIA is not a splice variant but a product of posttranslational proteolytic processing. PMID: 17341477
  29. Carditis and fibrosis in FXIII-deficient mice might be caused by a faulty or delayed reparative process that was initiated by abnormal haemorrhagic events within heart tissue. PMID: 18278192
  30. FXIIIa supports the early survival of micrometastases by a mechanism linked to NK cell function PMID: 18315549

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Subcellular Location
Cytoplasm. Secreted.
Protein Families
Transglutaminase superfamily, Transglutaminase family
Database Links
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