The Mouse Collagen Type I (Col I) ELISA Kit is specifically designed to standardize the detection of mouse Col I in multiple biological solutions, including serum, plasma, cell culture supernates, and tissue homogenates. This kit employs the Sandwich-ELISA mechanism to quantitatively measure the concentration of Col I. The Col I in the sample and standard is bound in a "sandwich" format by the capture antibody pre-coated on the microwell plate wells and subsequently added Biotin-labeled Col I antibody. The HRP-avidin is added and conjugated to the biotinylated Col I antibody, allowing for a colorimetric reaction to ensue upon TMB substrate addition. HRP-TMB interaction yields a blue color. The color development is terminated, and solution color changes from blue to yellow. The intensity is a positive representative to the Col I concentration.
This kit has been professionally verified with reliable quality, high sensitivity, strong specificity, good linearity, recovery of 87-107%, precision less than 10%, and consistency between batches. See more details on the product instructions.
Collagen type I is the most abundant extracellular matrix protein. It is made up of two α1(I) chains and one α2(I) chain. Apart from cartilaginous tissues, collagen type I is present throughout the body, including skin, tendons, vasculature, as well as organs such as lungs, and heart. It also forms the main component in the organic portion of the calcified tissue of bone and teeth. The interaction between collagen type I and integrin α2β1 is essential for bone-resorbing cells and osteoclasts.