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| Code | CSB-E08082h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 91-110 | |||||
| 1:4 | Average % | 105 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-100 | |||||
| The recovery of human Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-99 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This Human Col Ⅰ ELISA Kit was designed for the quantitative measurement of Human Col Ⅰ protein in serum, urine, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.
Sample type: Cell culture supernatant
Sample species: Human
Sample dilution: 1:5
Review: The collagen 1 content in cells was detected using the CSB-E08082h kit with a correct trend of the calibration curve and intact data.
By Anonymous
Sample type: Cell culture supernatant
Sample species: Human
Sample dilution: No dilution
Review: CSB-E08082h was used to detect exosomes promoting collagen type I production in fibroblasts, and its OD value is shown in the attached graph. The instruction manual is very detailed in the process of using, and it is very smooth to do the experiment according to the steps.
By Anonymous
