Mouse Collagen alpha-1(I) chain (COL1A1) ELISA kit

Code CSB-EL005727MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse COL1A1 ELISA Kit was designed for the quantitative measurement of Mouse COL1A1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Target Name collagen, type I, alpha 1
Alternative Names Col1a1 ELISA kit; Cola1Collagen alpha-1(I) chain ELISA kit; Alpha-1 type I collagen ELISA kit
Abbreviation COL1A1
Uniprot No. P11087
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse COL1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 81-92  
1:2 Average % 102  
Range % 95-105  
1:4 Average % 91  
Range % 85-95  
1:8 Average % 97  
Range % 91-101  
Recovery
The recovery of mouse COL1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 88 82-92  
EDTA plasma (n=4) 100 90-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.558 2.599 2.579 2.386  
10 2.008 2.111 2.060 1.867  
5 1.389 1.342 1.366 1.173  
2.5 0.843 0.828 0.836 0.643  
1.25 0.503 0.540 0.522 0.329  
0.625 0.377 0.384 0.381 0.188  
0.312 0.278 0.268 0.273 0.080  
0 0.195 0.191 0.193    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Type I collagen is a member of group I collagen (fibrillar forming collagen).
Gene References into Functions
  1. Our studies demonstrate that a collagen-I-dense ECM can potently alter hormonal signals to drive the progression of ERalpha + breast cancer, increasing intravasation and pulmonary metastases. PMID: 28103936
  2. Offspring that inherited the osteogenesis imperfect (OI) and high bone mass alleles had higher bone mass and strength than mice that inherited the OI allele alone. PMID: 27297606
  3. a surface population of Hsp90 extracellularly binds TGFbetaRI and this complex behaves as an active participant in collagen production in TGFbeta-activated fibroblasts. PMID: 27418101
  4. Gremlin1 accelerates hepatic stellate cell activation through upregulation of TGF-B1, alpha-SMA, and COL1a1 expression in a liver fibrosis disease model. PMID: 28467108
  5. collagen-I-mediated inhibition of proplatelet formation is specifically controlled by GPVI. PMID: 27505889
  6. Col1a1Jrt/+ mutant mice produce craniofacial and dental defects consistent with osteogenesis imperfecta and Ehlers-Danlos syndrome PMID: 26951553
  7. the rate of collagen I degradation was increased in Poldip2(+/-) vs. Poldip2(+/+) MASMs. Conversely, activation of the PI3K/Akt/mTOR signaling pathway, involved in regulation of protein synthesis, was significantly elevated in Poldip2(+/-) MASMs as was beta1-integrin expression. PMID: 26801741
  8. These data provided a plausible mechanism linking maternal vitamin D deficiency with altered postnatal lung function. PMID: 27121020
  9. Col1 expression was seen in the cartilage zone of the mandibular condyle cartilage. PMID: 26362410
  10. the Col1a1Jrt/+ mouse model of severe OI has hypersensitivity to mechanical and thermal stimuli, consistent with a state of chronic pain PMID: 26277094
  11. HRG binds to alpha2 integrin through low-affinity interactions in a heparin sulfate-independent manner, thereby blocking endothelial cells adhesion to collagen I. PMID: 26051322
  12. Calreticulin in mediates vascular smooth muscle cell responses to injury through the regulation of collagen deposition and neointima formation. PMID: 26910059
  13. Homocysteine Induces Collagen I Expression by Downregulating Histone Methyltransferase G9a PMID: 26192994
  14. These data suggested that intestinal epithelial cells were injured after IM treatment through the pVHL overexpression-induced degradation of collagen I or HIF-1alpha. PMID: 26551465
  15. lipid nanoparticles loaded with small interfering RNA to the procollagen alpha1(I) gene specifically reduce total hepatic collagen content without detectable side effects, potentially qualifying as a therapy for fibrotic liver diseases PMID: 26096209
  16. Collagen Ialpha1 matricryptin p1158/59 facilitates left ventricle remodeling post-myocardial infarction by regulating scar formation through targeted extracellular matrix generation and stimulation of angiogenesis. PMID: 26383724
  17. low-level laser therapy influences the expression of col1a1 and vegf in a mouse fibroblast cell line. PMID: 25171833
  18. Hgh fat diet increased TGFbeta1 protein expression, Smad3 activation, and collagen deposition in skeletal muscle, and exercise attenuated TGFbeta1 protein expression and collagen deposition in skeletal muscle. PMID: 25689619
  19. During heart valve development, Krox20-mediated activation of fibrillar Col1a1 and Col3a1 genes is crucial to avoid postnatal degeneration of the aortic valve leaflets. PMID: 25344368
  20. Data indicate significantly reduced volumetric density of elastin and collagen and thinner skin dermis were observed in Marfan mice. PMID: 25086405
  21. Fibrocytes are not an essential source of type I collagen during lung fibrosis. PMID: 25281715
  22. Allele-specific Col1a1 silencing reduces mutant collagen in fibroblasts from Brtl mouse, a model for classical osteogenesis imperfecta. PMID: 24022296
  23. These findings reveal a novel aging cascade whereby resistance to collagen cleavage accelerates cellular aging. PMID: 23957394
  24. Hypoxic tumor environments exhibit disrupted collagen I fibers and low macromolecular transport. PMID: 24349142
  25. results indicate that Hrd1 plays an important role in the maturation of collagen I in renal fibrosis, and that Sec23A pathway is required for ER-to-Golgi procollagen trafficking to promote collagen synthesis PMID: 24114659
  26. Data indicate a significantly increased expression of type I collagen, TIMP-2, TGF-beta, PAI-1 and RAGE in diabetic db/db cells. PMID: 23991045
  27. Analysis of mutant mouse lines for Col1a1 and Jag1 (Delta-Notch pathway) that both showed clear alterations in several bone-related parameters PMID: 23371561
  28. the absence of integrin alpha2beta1 alleviates the effects of age related bone degradation through over-expression of collagen type I. PMID: 23680479
  29. Data indicate serine-threonine kinase receptor-associated protein (STRAP) as a novel regulator of the coordinated translation of collagen alpha1(I) and alpha2(I) mRNAs. PMID: 23918805
  30. This study deministrated that collagen1alpha1 cells as a novel source of the fibrotic scar after spinal cord injury and shift the focus from the meninges to the vasculature during scar formation. PMID: 23966707
  31. DNA injection and electroporation of the testes showed that spermatogonia self-renewal ability was impaired remarkably with the loss-of-function of Col1a1. PMID: 23064687
  32. Strong transgenerational inheritance of these perturbations was observed only at transgenes integrated at the Col1a1 locus, where both activating and repressive chromatin modifications were heritable for multiple generations. PMID: 23325759
  33. Trps1 is identified as a potent inhibitor of Dspp expression and the subsequent mineralization of dentin. PMID: 22508542
  34. Hypermethylation of CpG sites in the promoter/exon 1 of COL1A1 may underlie reduced collagen synthesis at the transcriptional level in myopic scleras. PMID: 22690110
  35. we show that Prdm5 controls both Collagen I transcription and fibrillogenesis by binding inside the Col1a1 gene body and maintaining RNA polymerase II occupancy PMID: 22589746
  36. Higher MMP2 expression in mice on high-fat diet suggests its involvement in the reduction of COL1A1 protein abundance with high-fat diet. PMID: 21775118
  37. LH3-mediated glucosylation occurs at the specific molecular loci in the type I collagen molecule and plays critical roles in controlling collagen cross-linking, fibrillogenesis, and mineralization. PMID: 22573318
  38. atomistic simulations of a section of mouse type I heterotrimer and homotrimer collagen molecules PMID: 22325288
  39. Adult male offspring of OVA-sensitized /challenged dams exposed to maternal cigarette smokeS (OSM) displayed 1.8-fold greater collagen 1A1 mRNA levels. PMID: 21718087
  40. Fetal microchimeric cells switch on collagen type I transcription implicating mesenchymal lineage cells. PMID: 21150166
  41. study reports that vimentin filaments associate with collagen mRNAs in a 5'stem-loop sequence and LARP6-dependent manner and stabilize collagen mRNAs PMID: 21746880
  42. an important role of the 5' stem-loop in regulating type I collagen mRNA and protein expression PMID: 21193410
  43. Periostin is a PTH regulated extracellular matrix protein, partly co-localizing with collagen 1a1. PMID: 20654714
  44. SPARC is a critical regulator of collagen assembly. The type I collagen N-propeptide can effect the incorporation of collagen I into fibrillar extracellular matrix. PMID: 20708079
  45. Col(I)-Luc transgenic mice can be applied in the field of bone tissue engineering for monitoring bone repair processes and for investigating osteoinductive molecules or scaffolds. PMID: 20218816
  46. Transgenic (col1A1) Col3.6-HSD2 mice showed a low bone mass phenotype, with decreased ex vivo osteogenesis, which strengthens the concept that endogenous glucocorticoid signaling is required for optimal bone mass acquisition PMID: 20541046
  47. Exogenous VEGF restored resistance to oxidative stress in BAV SMCs to normal. Type I collagen gene induction was increased in BAV aorta. PMID: 21095332
  48. Demonstrate that collagen content is important to extralobar pulmonary artery stiffening caused by chronic hypoxia in Col1a1 transgenic mice. PMID: 20852040
  49. Interactions of alpha1(I)(3) homotrimers and normal alpha1(I)(2)alpha2(I) heterotrimers with fibroblast collagenase (MMP-1), were studied. PMID: 20463013
  50. Col1a1 gene regulatory elements have sites of stress-induced DNA duplex destabilization and nuclear scaffold/matrix association potential PMID: 11813254

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Subcellular Location Secreted, extracellular space, extracellular matrix.
Protein Families Fibrillar collagen family
Tissue Specificity Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
Database Links

KEGG: mmu:12842

STRING: 10090.ENSMUSP00000001547

UniGene: Mm.277735

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