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24T ELISA Kit Trial Size (Only USD$150/ kit)
|Intra-assay Precision (Precision within an assay): CV%<8%|
|Three samples of known concentration were tested twenty times on one plate to assess.|
|Inter-assay Precision (Precision between assays): CV%<10%|
|Three samples of known concentration were tested in twenty assays to assess.|
|To assess the linearity of the assay, samples were spiked with high concentrations of mouse orexin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.|
|The recovery of mouse orexin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.|
|Sample Type||Average % Recovery||Range|
|EDTA plasma (n=4)||100||89-103|
|These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.|
The Mouse Orexin A ELISA Kit is a powerful tool for quantitative analysis of Orexin A protein levels in mouse serum, plasma, tissue homogenates, and cell lysates. Orexin A, also known as hypocretin-1, is a neuropeptide hormone that plays a crucial role in regulating sleep-wake cycles, appetite, and energy metabolism.
The Mouse Orexin A ELISA Kit has a wide detection range of 78 pg/mL-5000 pg/mL and a high sensitivity of 19.5 pg/mL, ensuring precise and accurate measurements even at low concentrations. The kit uses a sandwich assay principle, which involves the use of two monoclonal antibodies specific to the Orexin A protein.
The assay time for the kit is 1-5 hours, and the sample volume required is 50-100ul. Detection is done at a wavelength of 450 nm, making it easy to read and interpret the results. The kit is suitable for use in metabolism research.
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