Mouse Protein Wnt-7b(WNT7B) ELISA kit

Instructions
Code CSB-EL026142MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name wingless-type MMTV integration site family, member 7B
Alternative Names Wnt7b ELISA Kit; Wnt-7b ELISA Kit; Protein Wnt-7b ELISA Kit
Abbreviation WNT7B
Uniprot No. P28047
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Stem Cells
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT7B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %93
Range %85-105
1:2Average %89
Range %84-96
1:4Average %95
Range %90-100
1:8Average %95
Range %87-101
Recovery
The recovery of mouse WNT7B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9793-101
EDTA plasma (n=4)9489-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.580 2.618 2.599 2.447
101.532 1.482 1.507 1.355
50.933 0.895 0.914 0.762
2.50.579 0.586 0.583 0.431
1.250.352 0.346 0.349 0.197
0.6250.258 0.269 0.264 0.112
0.3120.207 0.213 0.210 0.058
00.155 0.149 0.152
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Gene References into Functions
  1. Ptgs2 inhibitor celecoxib reduces dsRNA-induced WIHN and Wnt7b, and exogenous prostaglandin E2 can rescue WIHN and Wnt7b. Although other Wnts and pathways likely contribute, these results highlight noncoding dsRNA as an upstream coordinator of prostaglandin and Wnt levels in regeneration. PMID: 28392344
  2. In a mouse model of myopia, there was downregulation of WNT7B expression in the cornea and upregulation in the retina, suggesting its possible role in the development of myopia. PMID: 25823570
  3. Wnt7b is required for epithelial progenitor growth and operates during epithelial-to-mesenchymal signaling in pancreatic development. PMID: 25576928
  4. Transgenic Wnt7b overexpression in neural progenitor cells impairs neuronal differentiation and the development of forebrain structures at embryonic day 10.5. It decreased expression of Tbr1 and Tbr2, in both progenitor cells and post-mitotic neurons. PMID: 24953933
  5. These findings demonstrate the importance of intrinsic Wnt7b expression in hfSCs regulation and normal HF cycling PMID: 24222445
  6. Stable ectopic expression of Wnt-7b in OSCC cells overexpressing miR329 or miR410 restored proliferation and invasion capabilities abolished by these miRNA PMID: 25351956
  7. Results illustrated the critical role of myeloid WNT7B in tumor progression, acting at the levels of angiogenesis, invasion, and metastasis. PMID: 24638982
  8. Thus, WNT7B promotes bone formation in part through mTORC1 activation. PMID: 24497849
  9. Wnt7b preserves endothelial phenotype in aortic endothelial cells. PMID: 23685555
  10. Epithelial Wnt7b and Wnt9b as possible ligands of Fzd1-mediated beta-catenin (Ctnnb1)-dependent (canonical) Wnt signaling in the undifferentiated ureteric mesenchyme. PMID: 22833126
  11. Dkk1 controls the degree of Wnt-7b signalling in the papilla to coordinate kidney organogenesis. PMID: 21354128
  12. Because Wnt7b is known to stimulate epithelial responses during kidney development, these findings suggest that macrophages are able to rapidly invade an injured tissue and reestablish a developmental program that is beneficial for repair and regeneration PMID: 20160075
  13. promoter is regulated by TTF-1, GATA6, and Foxa2 in lung epithelium PMID: 11914369
  14. Wnt7b signaling is required for proper lung mesenchymal growth and vascular development PMID: 12361974
  15. Data demonstrate that Wnt7b signals through Fzd1 and -10 and LRP5 and implicate these Wnt coreceptors in the regulation of lung airway and vascular development. PMID: 15923619
  16. macrophage WNT7b is a short-range paracrine signal required for WNT-pathway responses and programmed cell death in the vascular endothelial cells of the temporary hyaloid vessels of the developing eye PMID: 16163358
  17. Wnt7b signals to neighboring cells to activate both autocrine and paracrine canonical Wnt signaling cascades. In contrast to results from hypomorphic models, Wnt7b modulates several important signaling pathways in the lung. PMID: 18367557
  18. These findings demonstrate that Wnt7b is a key regulator of the tissue architecture that establishes a functional physiologically active mammalian kidney. PMID: 19060336
  19. alpha3beta1 integrin, a major laminin receptor, and c-Met, the receptor for hepatocyte growth factor, signal coordinately to regulate the expression of Wnt7b in the papilla of the developing kidney PMID: 19176588
  20. Wnt7b regulates a program of mesenchymal differentiation in the lung that is essential for SMC development. PMID: 19690384

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Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Wnt family
Database Links

KEGG: mmu:22422

STRING: 10090.ENSMUSP00000105051

UniGene: Mm.306946

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