Mouse brain natriuretic peptide,BNP ELISA Kit

Code CSB-E07971m
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
natriuretic peptide precursor B
Alternative Names
NppbNatriuretic peptides B ELISA kit; Gamma-brain natriuretic peptide) [Cleaved into: Brain natriuretic peptide ELISA kit; BNP)] ELISA kit
Uniprot No.
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates, cell culture supernates
Detection Range
6.25 pg/mL-400 pg/mL
1.56 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse BNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %92
Range %86-98
1:2Average %96
Range %90-102
1:4Average %103
Range %100-107
1:8Average %89
Range %84-95
The recovery of mouse BNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9290-95
EDTA plasma (n=4)103101-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
4002.877 2.984 2.931 2.774
2002.136 2.015 2.076 1.919
1001.322 1.433 1.378 1.221
500.811 0.862 0.837 0.680
250.512 0.514 0.513 0.356
12.50.322 0.302 0.312 0.155
6.250.240 0.250 0.245 0.088
00.153 0.160 0.157
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Mouse NPPB ELISA Kit was designed for the quantitative measurement of Mouse NPPB protein in serum, plasma, tissue homogenates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

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Is the plate in this kit strip type?

Thanks for your inquiry. The plate of 96T kit is 8wells*12 strips.
For further questions,just let me know.

Is the standard for this kit mouse cyclized 45 amino acid form? It is critical for our studies that the assay is for this form and not linear 1-32 form or prohormone.


The standard is 293 cell-derived recombinant protein.

Target Background

(From Uniprot)
Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Likely involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, kaluresis and chloruresis.
Gene References into Functions
  1. These results suggested that the papillary tip express a substance that can stimulate BNP production and secretion from cardiomyocytes. PMID: 29734386
  2. The present study provides a novel insight into the potential underlying mechanisms of telmisartan-induced inhibition of cardiomyocyte hypertrophy, which involves inhibition of NFAT activation, nuclear translocation and the ANP/BNP cascade. PMID: 28447738
  3. the developmental regulation and stress response of the Nppa-Nppb cluster involve the concerted action of multiple enhancers and epigenetic changes distributed across a structurally rigid regulatory domain. PMID: 27048739
  4. Data suggest that the negative inhibition of P2X3R activity by the BNP/NPR-A pathway results in a decreased P2X3R-mediated excitability of trigeminal neurons in wildetype cultures. In familial hemiplegic migraine type-1 model cultures, however, lack of efficient P2X3Rs downregulation contributes to the neuronal hyperexcitability phenotype. PMID: 27346147
  5. P2X3 receptors on mouse trigeminal ganglion neurons are subjected to contrasting modulation by inhibitory brain natriuretic peptide and facilitatory calcitonin gene-related peptide that both operate via complex intracellular signaling. PMID: 27175010
  6. mouse trigeminal neurons endogenous BNP acts on NPR-A receptors to determine constitutive depression of P2X3 receptor function. Tonic inhibition of P2X3 receptor activity by BNP/NPR-A/PKG pathways occurs via two distinct mechanisms: P2X3 serine phosphorylation and receptor redistribution to non-raft membrane compartments PMID: 26576636
  7. Data (including data from studies in knockout mice) suggest expression of ANP/BNP/GC-A (atrial natriuretic peptide, brain natriuretic peptide, and guanylyl cyclase-A) is important in embryonic neovascularization and organogenesis. PMID: 26517044
  8. Goalpha plays a role in ANF sorting during intracellular vectorial transport and with the presence of a mechanism that preserves the molar relationship between cellular ANF and BNP. PMID: 25917834
  9. The RNA-Seq demonstrates that NPPB which well activates natriuretic peptide receptor 1 (NPR1), is well expressed in mouse dorsal root ganglian. PMID: 25123163
  10. a slow modulatory action by BNP on TRPV1 and P2X3 receptors outlining the role of this peptide as a negative regulator of trigeminal sensory neuron excitability to nociceptive stimuli PMID: 24312267
  11. These data provide detailed novel insight into the complex effects of natriuretic peptides and their receptors on electrical conduction in the heart. PMID: 24344164
  12. Natriuretic peptides buffer renin-dependent hypertension. PMID: 24717731
  13. BNP-NPRA signaling is involved in both itch and pain and does not function upstream of the GRP-GRPR dedicated neuronal pathway. PMID: 24438367
  14. Data indicate that the identified stress-responsive enhancer of Nppa and Nppb, the most common markers of heart failure, is a 650-bp fragment within 50 kb of the Nppa and Nppb loci. PMID: 24391132
  15. these results define the primary pruriceptive neurons, characterize Nppb as an itch-selective neuropeptide, and reveal the next two stages of this dedicated neuronal pathway. PMID: 23704570
  16. Elevated BNP levels, by inducing sympathetic overdrive and altering Ca(2+) handling, promote adverse cardiac remodelling and ventricular arrhythmias PMID: 22617407
  17. BNP controls early load-dependent regulation of SERCA through calcineurin in heart failure. PMID: 20711735
  18. Expression of brain natriuretic peptide and other serum response factor-dependent fetal cardiac genes in response to acute mechanical stress was blunted in mice lacking Mrtf-A. PMID: 20606005
  19. plasma levels of adrenomedullin, atrial natriuretic peptide(ANP)and BNP increased in association with aging; ANP and BNP increased in association with pulse pressure; possible relation between levels and age-related changes in cardiovascular system PMID: 12484513
  20. BNP-mRNA expression in normotensive AT2-deficient mice demonstrate a direct interaction of the renin-angiotensin system and natriuretic peptide that is fibrosis- rather than blood pressure-dependent. PMID: 14599720
  21. activation of cardiac GC-A by locally secreted atrial and brain natriuretic peptides protects the heart from excessive cardiac remodeling by inhibiting the calcineurin-NFAT pathway. PMID: 15939815
  22. ET-1 and Ang II play a key role in the induction of B-type natriuretic peptide in response to load in intact adult murine hearts. PMID: 17188849
  23. BNP is a specific HIF-1alpha target gene PMID: 17822384
  24. mephrin A initially truncated BNP in kidney and the truncated BNP was catabolized by neprilysin PMID: 17823376
  25. The results show that B-type natriuretic peptide decreases intragastric pressure, emptying and absorption by acting via the NPR-A receptor. PMID: 18367637
  26. BNP promotes vessel growth by increasing the number of endothelial progenitors and enhancing their functional properties. PMID: 19275472
  27. endogenous ANP/BNP protects the heart against the death and progression of pathological remodeling in a mouse model of dilated cardiomyopathy and sudden death PMID: 19346456
  28. Targeting of BNP with short interfering RNA (siRNA) resulted in the inhibition of embryonic stem cell proliferation, as indicated by a marked reduction in the cell number and colony size. PMID: 19399180
  29. Data indicate that EET-mediated cardioprotection involves BNP and PI3K signalling events. PMID: 19401302
  30. The natriuretic peptide/guanylyl cyclase--a system functions as a stress-responsive regulator of angiogenesis in mice. PMID: 19487812
  31. Data show that the system was able to measure the induction of hypertrophic markers BNP and ANP in tissue after 2 hrs and 5 hrs of stretch. PMID: 19670825

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Subcellular Location
[Brain natriuretic peptide 45]: Secreted.
Protein Families
Natriuretic peptide family
Tissue Specificity
[Brain natriuretic peptide 45]: Expressed abundantly in the ventricle, and in a lesser extent in the atrium (at protein level).
Database Links
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