Mouse glutathione peroxidase(GSH-Px)ELISA Kit

Instructions
Code CSB-E13068m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name glutathione peroxidase(GSH-Px)
Alternative Names Gpx1 ELISA Kit; Glutathione peroxidase 1 ELISA Kit; GPx-1 ELISA Kit; GSHPx-1 ELISA Kit; EC 1.11.1.9 ELISA Kit; Cellular glutathione peroxidase ELISA Kit; Selenium-dependent glutathione peroxidase 1 ELISA Kit
Abbreviation GSH-Px
Uniprot No. P11352
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 U/mL-2000 U/mL
Sensitivity 7.8 U/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse GSH-PX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 91
Range % 85-97
1:2 Average % 94
Range % 89-98
1:4 Average % 97
Range % 91-102
1:8 Average % 95
Range % 89-99
Recovery
The recovery of mouse GSH-PX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 93-102
EDTA plasma (n=4) 88 82-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
U/ml OD1 OD2 Average Corrected
2000 2.324 2.401 2.363 2.275
1000 1.602 1.699 1.651 1.563
500 1.056 1.039 1.048 0.960
250 0.657 0.665 0.661 0.573
125 0.431 0.446 0.439 0.351
62.5 0.274 0.285 0.280 0.192
31.25 0.158 0.162 0.160 0.072
0 0.087 0.088 0.088  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Protects the hemoglobin in erythrocytes from oxidative breakdown.
Gene References into Functions
  1. This repletion study shows that loss of capacity to incorporate Se into Gpx1 in Gpx1 KO mice does not dramatically alter expression of other Se biomarkers, nor the short-term flux of Se from intestine to liver to kidney. PMID: 29502249
  2. interactive modulations between the GPx-1 gene and Nrf2-dependent glutathione induction are critical for attenuating phencyclidine-induced abnormal behaviors in mice. PMID: 27796745
  3. Notably, CUEDC2 promoted E3 ubiquitin ligases tripartite motif-containing 33 (TRIM33)-mediated the antioxidant enzyme, glutathione peroxidase 1 (GPX1) ubiquitination, and proteasome-dependent degradation. PMID: 27286733
  4. GPx1 does not clearly exacerbate hyperoxia-induced increases in oxidative stress or lung injury but may alter pulmonary immune function. PMID: 28385808
  5. GPx-1 expression deters the unfolded protein response following exposure to cigarette smoke PMID: 28070146
  6. Glutathione peroxidase 1 (GPx1) knockdown not only induced oxidative stress characterized by the increased production of reactive oxygen species (ROS) but also caused reductive stress indicated by an elevation of glutathione (GSH)/oxidized GSH (GSSG) ratio. PMID: 28039148
  7. Exposure to far infrared rays significantly protects acute restraint stress oxidative burdens via inhibition of JAK2/STAT3 signaling by induction of GPx-1. PMID: 26850477
  8. Genetic inhibition of Gpx1 potentiates cocaine-induced renal damage via activation of AT1R by inhibition of PI3K-Akt signaling. PMID: 26930476
  9. Glutathione peroxidase 1 deficiency attenuates concanavalin A-induced liver injury by induction of T-cell hyporesponsiveness through chronic reactive oxygen species exposure. PMID: 27124582
  10. GPx1 was found to play a critical role in regulating pro-inflammatory pathways in vascular endothelium. PMID: 26569096
  11. Plasmalogen enrichment via batyl alcohol supplementation attenuated atherosclerosis in ApoE- and ApoE/GPx1-deficient mice. PMID: 26545014
  12. Gpx1 expression in the mouse skeletal muscle can be altered by both exercise and dyslipidemia through changes in DNA methylation, leading to a fine regulation of free radical metabolism. PMID: 27010651
  13. our findings unveil a new metabolic role for Reg3beta in protein nitration and a new biosynthesis control of GPX1 by a completely "unrelated" regenerating protein, Reg3beta, via transcriptional activation of Scly PMID: 23811004
  14. Rora induces the mRNA level of antioxidant enzymes, superoxide dismutase 2 and glutathione peroxidase 1, through the Rora response elements located in the upstream promoters of Sod2 and Gpx1. PMID: 24597775
  15. High-fat-fed Gpx1(-/-) mice also exhibited decreased hepatic steatosis and liver damage accompanied by decreased plasma insulin and decreased glucose-induced insulin secretion. PMID: 24252128
  16. we propose that ileocolitis in the DKO mice is caused by Nox1, which is induced by TNF. The milder disease in female het-TKO intestine is probably due to random or imprinted X-chromosome inactivation, which produces mosaic Nox1 expression. PMID: 24374371
  17. PD-linked mutations in Parkin and DJ-1 cause dysregulation of neurotransmitter systems beyond the nigrostriatal dopaminergic circuit especially in the context of Gpx1 deficiency. PMID: 24075852
  18. During early differentiation of embryonic stem (ES) cells, the quick degradation of GPx-1 was mediated by proteasome. Both knockdown of GPx-1 expression with shRNA and inhibiting GPx-1 activity by inhibitor led to the differentiation of ES cells. PMID: 24802396
  19. Data indicate that he macrophage-colony-stimulating factor (MCSF) and oxLDL-induced proliferation of peritoneal macrophages from GPx-1(-/-)ApoE(-/-) mice was mediated by the ERK1/2 (extracellular-signal regulated kinase 1/2) signaling pathway. PMID: 23991041
  20. The present study has sought to investigate the extent of vascular dysfunction and oxidant formation in glutathione peroxidase-1-deficient (GPx-1(-/-)) mice during the aging process with special emphasis on dysregulation of the endothelial NO synthase. PMID: 24296279
  21. GPx-1-PTP1B-PP2A axis plays a critical role in countering the inflammatory and proteolytic responses that result in lung-tissue destruction in response to cigarette smoke exposure. PMID: 23590304
  22. How does oxidative stress contribute to vascular disease? Data from vascular smooth muscle suggest that reduced GPx1 activity (i.e., increased oxidative stress) promotes secretion of cyclophilin A which mediates paracrine inflammation of vasculature. PMID: 21782974
  23. Candidate colitis genes in the Gdac1 locus of mice deficient in glutathione peroxidase-1 and -2, were analyzed. PMID: 22970191
  24. GPx1, an important antioxidant enzyme of the premature retina, afforded protection against oxidative stress and oxidative injury in ROP. PMID: 23287791
  25. Dietary selenium deficiency precluded the overproduction of glutathione peroxidase-1 in full-fed over-expressing mice and partially rescued their metabolic syndromes. PMID: 23014491
  26. The peroxidase activity is the main mechanism for Prdx6 antioxidant function, these results indicate that the PLA(2) activity also plays a substantial role in protecting cells against oxidant stress caused by an exogenous hydroperoxide. PMID: 22067043
  27. gpx-1 primes pro-inflammatory cytokine production after LPS challenge in vivo. PMID: 22412999
  28. Gpx1 mRNA expression is higher in female than male early after birth. PMID: 22326323
  29. Our data reveal a structural and kinetic mechanism for the in vivo functional dependence of GPX1 on SOD1 in mammals. PMID: 21420488
  30. The data indicate glutathione peroxidase GPX1 levels is decreased in stem cell-like characteristics. PMID: 21347610
  31. molecular events contributing to neuron injury after transient cerebral ischemia; data imply absence of Gpx1 induces activation of compensatory signaling mechanisms reducing oxidative stress without potentiating transduction of existing activated pathways PMID: 21193029
  32. GPx1-dependent control of intracellular ROS accumulation is important not only for regulation of Th cell proliferation but also for modulation of differentiation into Th1, Th2, and Th17 cells. PMID: 20367278
  33. Data show that dietary selenium deficiency exhibits a hypoinsulinemic trend in GPX1-overexpressing mice and a strong hypolipidemic effect in the liver of WT mice. PMID: 20578960
  34. Data show that knockout of SOD1 impaired islet function, pancreas integrity, and body glucose homeostasis more than that of GPX1. PMID: 20586612
  35. Gpx1 regulates leukocyte-endothelial cell interactions in the cerebral microvasculature, but that this is affected by the nature of the inflammatory insult. PMID: 21166922
  36. Loss of Gpx1 is associated with diet induced severe colitis. PMID: 20848490
  37. In GPx2 KO mice, an increase in GPx1 can only partially compensate for GPx2, even under selenium supplementation, indicating that GPx2 is the major antiapoptotic GPx in the colon. PMID: 20828612
  38. knockout of SOD1 and (or) GPX1 did not potentiate the LPS-induced liver injury, but delayed the induced hepatic glutathione depletion and plasma NO production. PMID: 21145306
  39. Gpx-1 protects against cigarette smoke-induced lung inflammation, and agents that mimic the actions of gpx-1 may have therapeutic utility in inflammatory lung diseases where cigarette smoke plays a role. PMID: 20511341
  40. Report the role of GPx1 in protecting cardiac mitochondria against reoxygenation damage in vivo. PMID: 20306076
  41. Glutathione peroxidase deficiency exacerbates ischemia-reperfusion injury in male but not female myocardium: insights into antioxidant compensatory mechanisms. PMID: 19801492
  42. Results indicate that Gpx1 deficiency accelerates cardiac hypertrophy and dysfunction but has no effect on vascular hypertrophy and mean arterial blood pressure PMID: 19917877
  43. Yam was found to increase the activities of superoxide dismutase and glutathione peroxidase in brain. PMID: 19885949
  44. In vivo antioxidant role of glutathione peroxidase: evidence from knockout mice. PMID: 11898410
  45. Glutathione peroxidase-1 has a role in modulating molecular pathways involved in both cell death and inflammatory cascades in brain following injury. PMID: 12429206
  46. Deficiency of GPx-1 exacerbates endothelial dysfunction in hyperhomocysteinemic mice and demonstrates support for the hypothesis that hyperhomocysteinemia contributes to endothelial dysfunction through a peroxide-dependent oxidative mechanism. PMID: 12482825
  47. Data show that phospho-c-Jun N-terminal kinase may catalyze phosphorylation of p53 protein in Se-deficient mouse liver, and that this cascade is attenuated by low levels of glutathione peroxidase 1. PMID: 12492400
  48. GPX1 can exert a potent role in defending against liver aponecrosis induced by moderate oxidative stress PMID: 12654481
  49. Gpx1 functions as the primary protection against acute oxidative stress PMID: 12804009
  50. Gpx1 is protective against both ROS-mediated senescence-like changes and oxidant-mediated cell death PMID: 14732290

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Subcellular Location Cytoplasm
Protein Families Glutathione peroxidase family
Database Links

KEGG: mmu:14775

STRING: 10090.ENSMUSP00000081010

UniGene: Mm.1090

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