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| Code | CSB-E04804m |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:10 | Average % | 88 | ||||
| Range % | 82-95 | |||||
| 1:20 | Average % | 97 | ||||
| Range % | 93-101 | |||||
| 1:40 | Average % | 99 | ||||
| Range % | 95-106 | |||||
| 1:80 | Average % | 93 | ||||
| Range % | 87-99 | |||||
| The recovery of mouse NE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-95 | ||||
| EDTA plasma (n=4) | 99 | 95-104 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The mouse neutrophil elastase (NE) ELISA Kit is engineered for accurate measurement of mouse NE levels from samples including serum, plasma, cell culture supernates, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the NE content in the sample. The color intensity is positively correlated with NE content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.
NE is a serine protease that plays a causal role in inflammation and is involved in various physiological processes such as the formation of neutrophil extracellular trap and degradation of extracellular matrix and proteins. It is mainly found in neutrophil azurophilic granules. NE can be rapidly released from neutrophils in response to inflammatory stimuli and is therefore regarded as a sign of inflammation and is closely associated with some inflammatory diseases such as bronchiectasis and chronic obstructive pulmonary disease. It also actively participates in cardiovascular disease. NE enhances myocardial damage by triggering excessive inflammation. And many studies have found that high expression of NE in atherosclerotic plaques.
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