Mouse neutrophil elastase,NE ELISA Kit

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Target Background

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Product Details

Description

The mouse neutrophil elastase (NE) ELISA Kit is engineered for accurate measurement of mouse NE levels from samples including serum, plasma, cell culture supernates, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the NE content in the sample. The color intensity is positively correlated with NE content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.

NE is a serine protease that plays a causal role in inflammation and is involved in various physiological processes such as the formation of neutrophil extracellular trap and degradation of extracellular matrix and proteins. It is mainly found in neutrophil azurophilic granules. NE can be rapidly released from neutrophils in response to inflammatory stimuli and is therefore regarded as a sign of inflammation and is closely associated with some inflammatory diseases such as bronchiectasis and chronic obstructive pulmonary disease. It also actively participates in cardiovascular disease. NE enhances myocardial damage by triggering excessive inflammation. And many studies have found that high expression of NE in atherosclerotic plaques.

Target Name

elastase 2, neutrophil

Alternative Names

Elane ELISA Kit; Ela2Neutrophil elastase ELISA Kit; EC 3.4.21.37 ELISA Kit; Elastase-2 ELISA Kit; Leukocyte elastase ELISA Kit

Abbreviation

ELA2

Uniprot No.

Q3UP87

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse NE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	88
	Range %	82-95
1:20	Average %	97
	Range %	93-101
1:40	Average %	99
	Range %	95-106
1:80	Average %	93
	Range %	87-99

Recovery

The recovery of mouse NE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	99	95-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.849	2.793	2.821	2.627
10	2.132	2.222	2.177	1.983
5	1.465	1.388	1.427	1.233
2.5	0.948	0.929	0.939	0.745
1.25	0.643	0.666	0.655	0.461
0.625	0.472	0.442	0.457	0.263
0.312	0.278	0.288	0.283	0.089
0	0.198	0.189	0.194

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Background

Function (From Uniprot)	Medullasin modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Capable of killing E.coli; probably digests outer membrane protein A (ompA) in E.coli.
Gene References into Functions	the impact of NE mutations that are typical of human congenital neutropenia in a murine model of in vitro and in vivo neutrophil differentiation PMID: 27942017 Levels of neutrophil elastase (NE) and the nuclear factors CCAAT/enhancer-binding protein alpha (C/EBPalpha) and growth factor-independent 1 (GFI-1) are elevated in hematopoietic stem and progenitor cells from high-fat diet (HFD)-fed mice, but mice lacking either NE or C/EBPalpha are resistant to HFD-induced myelopoiesis. PMID: 28202548 Sivelestat treatment significantly reduced the protein level, neutrophil count, neutrophil elastase activity in bronchoalveolar lavage fluid (BALF). PMID: 28300553 Serine protease required for resistance to microbial infection; contributes to pathogenessis in endotoxic shock PMID: 10714686 Neutrophils from neutrophil elastase-deficient mice show no defect in transendothelial migration PMID: 11994507 results of ultrasound studies using the elastase and AngII apoE in 4 biomechanical parameters: aneurysm diameter, aneurysm volume, circumferential cyclic strain, and blood flow velocity PMID: 26064906 Sirtinol is a novel HNE inhibitor and may have the potential for clinical application in the treatment of inflammatory lung diseases. PMID: 25666548 Increased ELANE activity was associated with development of autoimmune diabetes mellitus in NOD mice. PMID: 25092677 Neutrophil elastase is involved in the induction of microbicidal activities of neutrophils against Trypanosoma cruzi-infected macrophages. PMID: 24599360 contributes to clearance of Aspergillus fumigatus infections PMID: 24054721 In conclusion, sivelestat reduced radiationinduced lung injury in the mice by suppressing neutrophil elastase activity and excessive inflammatory reactions PMID: 23404144 Lung cancer promotion by inflammation is partly mediated by activation of the IL-8/CXCR2 pathway and subsequent recruitment of neutrophils and release of neutrophil elastase. PMID: 24321240 a neutrophil elastase inhibitor prevents bleomycin-induced pulmonary fibrosis in mice PMID: 22441751 During Pseudomonas aeruginosa infection, interleukin (IL)-1beta processing is dependent on neutrophil elastase and not NLRC4 or caspase-1. PMID: 23024281 Neutrophil elastase modulates cytokine expression: contribution to host defense against Pseudomonas aeruginosa-induced pneumonia. PMID: 22927440 NADPH oxidase in antimicrobial host defense against A. fumigatus and B. cepacia, whereas the proteases neutrophil elastase, cathepsin G, and lysosomal cysteine protease cathepsin C/ dipeptidyl peptidase I are dispensable PMID: 22163282 Cathepsin G and neutrophil elastase contribute to lung-protective immunity against mycobacterial infections in mice. PMID: 22461690 Neutrophil elastase cleaves the murine hemidesmosomal protein BP180/type XVII collagen and generates degradation products that modulate experimental bullous pemphigoid. PMID: 21979170 This is the first study showing a contribution of neutrophil-derived neutral serine proteases CG and NE to lung-protective immunity against focal pneumonia-inducing serotype 19 S. pneumoniae in mice. PMID: 21911460 The selective sensitivity of G913X Elane granulocytic cells to ER stress provides new and strong support for the unfolded protein response model of disease pathogenesis in severe congenital neutropenia. PMID: 21285438 neutrophil infiltration and neutrophil elastase (NE) activity are elevated in the chronic UVB-irradiated skin of hairless mice, and confirmed the involvement of NE in MMP activation PMID: 20965700 In the absence of a pathogen challenge, neutrophil-derived proteases and nucleosomes contribute to large vessel thrombosis, the main trigger of myocardial infarction and stroke. PMID: 20676107 Since NE is maximally produced in promyelocytes, this protease may play a role in APL pathogenesis by facilitating the leukemogenic potential of PML-RARalpha. PMID: 14636558 neutrophil elastase and cathepsin G are inhibited by PAI-1 mutants PMID: 15131125 Neutrophil elastase has a role in PML-retinoic acid receptor alpha activities in early myeloid cells PMID: 15601827 When challenged intraperitoneally, mice deficient in neutrophil elastase are markedly more susceptible to Klebsiella infection and death. PMID: 15661916 PECAM-1, alpha6 integrins and neutrophil elastase work together to mediate neutrophil transmigration PMID: 15840647 The results of the present study strongly suggest that neutrophil elastase may represent a common pathogenic link between emphysema and fibrosis. PMID: 16045796 Neutrophil elastase depends on serglycin proteoglycan for localization in granules. PMID: 17272511 This study describes for the first time a novel hierarchy among proteases whereby a serine protease NE up-regulates expression of MMPs and cathepsins. PMID: 17442971 Neutrophil elastase and proteinase 3 complexes have different interaction patterns with peptidic substrates. PMID: 18023421 Neutrophil elastase cleaves laminin-332 (laminin-5) generating peptides that are chemotactic for neutrophils. PMID: 18178964 required for neutrophil egression from the vasculature into the alveolar space; interfering with this process leads to neutrophil-related endothelial cell injury. PMID: 18276796 neutrophil elastase might decrease the pulmonary endothelial production of prostacyclin by inhibiting endothelial NO production PMID: 18323746 PR3 and neutrophil elastase enhance neutrophil-dependent inflammation by eliminating the local antiinflammatory activity of progranulin. Our results support the use of serine protease inhibitors as antiinflammatory agents. PMID: 18568075 Neutrophil elastase (NE) degrades the major outer membrane protein F (OmpF); a targeted deficiency in NE negates its antibacterial role in vitro and in a Pseudomonas aeruginosa pneumonia model. PMID: 18802098 during acute peritoneal inflammation, NE expression and activity increase gradually, facilitating leukocyte influx. PMID: 19088179 Evidence for a role of Ela2 in antimicrobial immunity and endotoxic shock PMID: 10714686 Evidence for a role of neutrophil elastase in bleomycin-induced fibrosis PMID: 11451907 Show More Hide All
Protein Families	Peptidase S1 family, Elastase subfamily
Database Links	KEGG: mmu:50701 STRING: 10090.ENSMUSP00000038925 UniGene: Mm.262194

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Check Code：

Code	CSB-E04804m
Size	96T,5×96T,10×96T
Trial Size
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.