Mouse neutrophil elastase,NE ELISA Kit

Instructions
Code CSB-E04804m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name elastase 2, neutrophil
Alternative Names Elane ELISA Kit; Ela2Neutrophil elastase ELISA Kit; EC 3.4.21.37 ELISA Kit; Elastase-2 ELISA Kit; Leukocyte elastase ELISA Kit
Abbreviation ELA2
Uniprot No. Q3UP87
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse NE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:10Average %88
Range %82-95
1:20Average %97
Range %93-101
1:40Average %99
Range %95-106
1:80Average %93
Range %87-99
Recovery
The recovery of mouse NE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9187-95
EDTA plasma (n=4)9995-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.849 2.793 2.821 2.627
102.132 2.222 2.177 1.983
51.465 1.388 1.427 1.233
2.50.948 0.929 0.939 0.745
1.250.643 0.666 0.655 0.461
0.6250.472 0.442 0.457 0.263
0.3120.278 0.288 0.283 0.089
00.198 0.189 0.194  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Medullasin modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (By similarity).
Gene References into Functions
  1. the impact of NE mutations that are typical of human congenital neutropenia in a murine model of in vitro and in vivo neutrophil differentiation PMID: 27942017
  2. Levels of neutrophil elastase (NE) and the nuclear factors CCAAT/enhancer-binding protein alpha (C/EBPalpha) and growth factor-independent 1 (GFI-1) are elevated in hematopoietic stem and progenitor cells from high-fat diet (HFD)-fed mice, but mice lacking either NE or C/EBPalpha are resistant to HFD-induced myelopoiesis. PMID: 28202548
  3. Sivelestat treatment significantly reduced the protein level, neutrophil count, neutrophil elastase activity in bronchoalveolar lavage fluid (BALF). PMID: 28300553
  4. Serine protease required for resistance to microbial infection; contributes to pathogenessis in endotoxic shock PMID: 10714686
  5. Neutrophils from neutrophil elastase-deficient mice show no defect in transendothelial migration PMID: 11994507
  6. results of ultrasound studies using the elastase and AngII apoE in 4 biomechanical parameters: aneurysm diameter, aneurysm volume, circumferential cyclic strain, and blood flow velocity PMID: 26064906
  7. Sirtinol is a novel HNE inhibitor and may have the potential for clinical application in the treatment of inflammatory lung diseases. PMID: 25666548
  8. Increased ELANE activity was associated with development of autoimmune diabetes mellitus in NOD mice. PMID: 25092677
  9. Neutrophil elastase is involved in the induction of microbicidal activities of neutrophils against Trypanosoma cruzi-infected macrophages. PMID: 24599360
  10. contributes to clearance of Aspergillus fumigatus infections PMID: 24054721
  11. In conclusion, sivelestat reduced radiationinduced lung injury in the mice by suppressing neutrophil elastase activity and excessive inflammatory reactions PMID: 23404144
  12. Lung cancer promotion by inflammation is partly mediated by activation of the IL-8/CXCR2 pathway and subsequent recruitment of neutrophils and release of neutrophil elastase. PMID: 24321240
  13. a neutrophil elastase inhibitor prevents bleomycin-induced pulmonary fibrosis in mice PMID: 22441751
  14. During Pseudomonas aeruginosa infection, interleukin (IL)-1beta processing is dependent on neutrophil elastase and not NLRC4 or caspase-1. PMID: 23024281
  15. Neutrophil elastase modulates cytokine expression: contribution to host defense against Pseudomonas aeruginosa-induced pneumonia. PMID: 22927440
  16. NADPH oxidase in antimicrobial host defense against A. fumigatus and B. cepacia, whereas the proteases neutrophil elastase, cathepsin G, and lysosomal cysteine protease cathepsin C/ dipeptidyl peptidase I are dispensable PMID: 22163282
  17. Cathepsin G and neutrophil elastase contribute to lung-protective immunity against mycobacterial infections in mice. PMID: 22461690
  18. Neutrophil elastase cleaves the murine hemidesmosomal protein BP180/type XVII collagen and generates degradation products that modulate experimental bullous pemphigoid. PMID: 21979170
  19. This is the first study showing a contribution of neutrophil-derived neutral serine proteases CG and NE to lung-protective immunity against focal pneumonia-inducing serotype 19 S. pneumoniae in mice. PMID: 21911460
  20. The selective sensitivity of G913X Elane granulocytic cells to ER stress provides new and strong support for the unfolded protein response model of disease pathogenesis in severe congenital neutropenia. PMID: 21285438
  21. neutrophil infiltration and neutrophil elastase (NE) activity are elevated in the chronic UVB-irradiated skin of hairless mice, and confirmed the involvement of NE in MMP activation PMID: 20965700
  22. In the absence of a pathogen challenge, neutrophil-derived proteases and nucleosomes contribute to large vessel thrombosis, the main trigger of myocardial infarction and stroke. PMID: 20676107
  23. Since NE is maximally produced in promyelocytes, this protease may play a role in APL pathogenesis by facilitating the leukemogenic potential of PML-RARalpha. PMID: 14636558
  24. neutrophil elastase and cathepsin G are inhibited by PAI-1 mutants PMID: 15131125
  25. Neutrophil elastase has a role in PML-retinoic acid receptor alpha activities in early myeloid cells PMID: 15601827
  26. When challenged intraperitoneally, mice deficient in neutrophil elastase are markedly more susceptible to Klebsiella infection and death. PMID: 15661916
  27. PECAM-1, alpha6 integrins and neutrophil elastase work together to mediate neutrophil transmigration PMID: 15840647
  28. The results of the present study strongly suggest that neutrophil elastase may represent a common pathogenic link between emphysema and fibrosis. PMID: 16045796
  29. Neutrophil elastase depends on serglycin proteoglycan for localization in granules. PMID: 17272511
  30. This study describes for the first time a novel hierarchy among proteases whereby a serine protease NE up-regulates expression of MMPs and cathepsins. PMID: 17442971
  31. Neutrophil elastase and proteinase 3 complexes have different interaction patterns with peptidic substrates. PMID: 18023421
  32. Neutrophil elastase cleaves laminin-332 (laminin-5) generating peptides that are chemotactic for neutrophils. PMID: 18178964
  33. required for neutrophil egression from the vasculature into the alveolar space; interfering with this process leads to neutrophil-related endothelial cell injury. PMID: 18276796
  34. neutrophil elastase might decrease the pulmonary endothelial production of prostacyclin by inhibiting endothelial NO production PMID: 18323746
  35. PR3 and neutrophil elastase enhance neutrophil-dependent inflammation by eliminating the local antiinflammatory activity of progranulin. Our results support the use of serine protease inhibitors as antiinflammatory agents. PMID: 18568075
  36. Neutrophil elastase (NE) degrades the major outer membrane protein F (OmpF); a targeted deficiency in NE negates its antibacterial role in vitro and in a Pseudomonas aeruginosa pneumonia model. PMID: 18802098
  37. during acute peritoneal inflammation, NE expression and activity increase gradually, facilitating leukocyte influx. PMID: 19088179
  38. Evidence for a role of Ela2 in antimicrobial immunity and endotoxic shock PMID: 10714686
  39. Evidence for a role of neutrophil elastase in bleomycin-induced fibrosis PMID: 11451907

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Protein Families Peptidase S1 family, Elastase subfamily
Database Links

KEGG: mmu:50701

STRING: 10090.ENSMUSP00000038925

UniGene: Mm.262194

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