Mouse tartrate-resistant acid phosphatase 5b(TRACP-5b) ELISA Kit

Instructions
Code CSB-E08492m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name acid phosphatase 5, tartrate resistant
Alternative Names Acp5 ELISA Kit; T5ap ELISA Kit; Trap ELISA Kit; Tartrate-resistant acid phosphatase type 5 ELISA Kit; TR-AP ELISA Kit; EC 3.1.3.2 ELISA Kit; Tartrate-resistant acid ATPase ELISA Kit; TrATPase ELISA Kit; Type 5 acid phosphatase ELISA Kit
Abbreviation ACP5
Uniprot No. Q05117
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.078 mIU/mL-5 mIU/mL
Sensitivity 0.02 mIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TRACP-5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %100
Range %96-104
1:2Average %98
Range %92-107
1:4Average %93
Range %89-97
1:8Average %95
Range %90-99
Recovery
The recovery of mouse TRACP-5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9792-102
EDTA plasma (n=4)9187-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/mlOD1OD2AverageCorrected
52.412 2.464 2.438 2.270
2.52.029 2.007 2.018 1.850
1.251.655 1.564 1.610 1.442
0.6251.173 1.129 1.151 0.983
0.3120.734 0.782 0.758 0.590
0.1560.491 0.456 0.474 0.306
0.0780.247 0.227 0.237 0.069
00.168 0.167 0.168  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Q&A and Customer Reviews

 Q&A
Q:

I’m searching for ELISA kits to detect TRAP in mouse plasma/serum and in culture supernatants of osteoclasts cultured on bovine bone slices.
I saw that Cusabio proposes a kit for the detection of TRAP with the reference CSB-E08492m.
Can you tell me if this kit is specific of TRAP5b produced by the osteoclasts (no detection of TRAP produced by other cells)?
Also, do you know if 100 μL of serum/plasma is necessary for the test or if a dilution of the samples is required?
Finally, as I would like to measure TRAP in culture supernatants, I was wondering if conditioned media (alpha-MEM containing inactivated FBS and penicillin/streptomycin) can be used as samples and if bovine TRAP coming from bone slices or FBS will be detected by the kit.
Thank you in advance for your consideration.

A:
Thanks for your inquiry.
Pls check our answer:
TRACP is a good marker of bone resorption and osteoclast activity.
TRACP especially in the presence of tracp-5a (mainly from macrophages) and tracp-5b (mainly from osteoclasts) existed in the blood.
The determination of tracp-5b can reflect osteoclast activity.
CSB-E08492m is specifically for TRACP-5b, properly used for the mouse matrix samples.
The common samples are serum and plasma. The sample adding volume is 100ul. There is no need to dilute.
As for the TRAP in culture supernatants, pls arrange the preliminary experiment. Pls kindly note detects the mouse TRACP-5b not TRAP.
Any question, pls feel free to contact me.

Target Data

Function May play a role in the process of bone resorption. The osteoclastic trap acts on nucleotide tri- and diphosphates with higher affinity, compared with other substrates.
Gene References into Functions
  1. TRAP and nidogen-2 co-localized in adipose tissue cells in situ. PMID: 25450682
  2. Uteroferrin is a cytokine secreted by uterine glands in response to progesterone that promotes fetal erythropoiesis at various stages of pregnancy. PMID: 25093463
  3. Supraphysiologic oscillatory fluid shear induces upregulation of osteoclastic activity, measured by tartrate-resistant acid phosphatase activity and formation of mineral resorption pits. PMID: 24275439
  4. Pax6 binds endogenously to the proximal region of the tartrate acid phosphatase (TRAP) gene promoter and suppresses nuclear factor of activated T cells c1 (NFATc1)-induced TRAP gene expression. PMID: 23990468
  5. Serum TRAP levels were lower in mice receiving eggshell calcium citrate malate than those receiving eggshells calcium malate. PMID: 23603074
  6. tartrate resistant acid phosphatase PMID: 23444125
  7. TRAP is not essential for the formation of the epiphyseal vascular network. PMID: 22579636
  8. dephosphorylation of Man6P-containing lysosomal proteins requires the concerted action of Acp2 and Acp5 and is needed for hydrolysis and removal of degradation products PMID: 22158965
  9. Accumulation of vesicles in the cytoplasm of mutant osteoclasts indicates a novel function for tartrate-resistant acid phosphatase in modulating intracellular vesicular transport in osteoclasts PMID: 12243748
  10. USF1 and USF2 play a functional role in RANKL-dependent TRAP expression during osteoclast differentiation. PMID: 12663664
  11. TRACP expression is a target for regulation by the macrophage/osteoclast transcription factor PU.1 and the osteoclast commitment factor MiTF PMID: 14584903
  12. YY1 plays a functional role in RANKL-mediated TRAP gene expression during osteoclast differentiation. PMID: 15563837
  13. distinct tartrate-resistant acid phosphatase promoter regions have distinct roles in gene regulation in transgenic mice PMID: 15590658
  14. MMP-2 and MMP-9 expression by TRAP positive mononuclear and multinucleated cells are involved in articular cartilage destruction in CIA. PMID: 15862156
  15. TRAP release is due to secretion, rather than cell death PMID: 16475168
  16. TRACP is important for polarizing responses in naive T cells to antigen-presented dendritic cells. PMID: 16939395
  17. both synthesis as well as degradation of collagen are increased when TRAP is absent in mice at 8 weeks and 6 months of age, showing that TRAP has an important role in the metabolism of collagen PMID: 17551769
  18. transcriptional activity of the Tracp gene, in pre-osteoclastic cells, is negatively regulated by the binding of PARP-1 protein to a potential consensus sequence located in its promoter region PMID: 18021007
  19. prolonged hypoxia induces the formation of TRAP-positive osteoclast-like cells, suggesting the occurrence of an autocrine mechanism for osteoclastogenesis PMID: 18056037
  20. Over-expression of monomeric tartrate resistant acid phosphatase, but not the dimeric form in adipose tissue leads to early onset spontaneous hyperplastic obesity PMID: 18320034
  21. central role for ACP5 in removal of the Man-6-P recognition marker from lysosomal proteins, which are then dephosphorylated PMID: 18940929
  22. in the absence of RANKL and osteoclast differentiation, STAT6 binds the TRAP promoter after IL-4 treatment and directly enhances TRAP expression PMID: 19801646

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Subcellular Location Lysosome
Tissue Specificity Characteristic constituent of osteoclasts.
Database Links

KEGG: mmu:11433

STRING: 10090.ENSMUSP00000065425

UniGene: Mm.46354

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