Mouse tartrate-resistant acid phosphatase 5b(TRACP-5b) ELISA Kit

Code CSB-E08492m
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q05117
Lead Time 3-5 working days
Abbreviation ACP5
Target Name acid phosphatase 5, tartrate resistant
Alias MGC117378, TRAP, TrATPase|tartrate resistant acid phosphatase 5|tartrate-resistant acid ATPase
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.078 mIU/mL-5 mIU/mL
Sensitivity 0.02 mIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TRACP-5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %100
Range %96-104
1:2Average %98
Range %92-107
1:4Average %93
Range %89-97
1:8Average %95
Range %90-99
Recovery
The recovery of mouse TRACP-5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9792-102
EDTA plasma (n=4)9187-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/mlOD1OD2AverageCorrected
52.412 2.464 2.438 2.270
2.52.029 2.007 2.018 1.850
1.251.655 1.564 1.610 1.442
0.6251.173 1.129 1.151 0.983
0.3120.734 0.782 0.758 0.590
0.1560.491 0.456 0.474 0.306
0.0780.247 0.227 0.237 0.069
00.168 0.167 0.168  
References
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Function May play a role in the process of bone resorption. The osteoclastic trap acts on nucleotide tri- and diphosphates with higher affinity, compared with other substrates.
Subcellular Location Lysosome
Tissue Specificity Characteristic constituent of osteoclasts.
Database Links

KEGG: mmu:11433

STRING: 10090.ENSMUSP00000065425

UniGene: Mm.46354

Pathway Osteoclast differentiation

Q&A and Customer Reviews

Q&A
Q:

I’m searching for ELISA kits to detect TRAP in mouse plasma/serum and in culture supernatants of osteoclasts cultured on bovine bone slices.
I saw that Cusabio proposes a kit for the detection of TRAP with the reference CSB-E08492m.
Can you tell me if this kit is specific of TRAP5b produced by the osteoclasts (no detection of TRAP produced by other cells)?
Also, do you know if 100 μL of serum/plasma is necessary for the test or if a dilution of the samples is required?
Finally, as I would like to measure TRAP in culture supernatants, I was wondering if conditioned media (alpha-MEM containing inactivated FBS and penicillin/streptomycin) can be used as samples and if bovine TRAP coming from bone slices or FBS will be detected by the kit.
Thank you in advance for your consideration.

A:
Thanks for your inquiry.
Pls check our answer:
TRACP is a good marker of bone resorption and osteoclast activity.
TRACP especially in the presence of tracp-5a (mainly from macrophages) and tracp-5b (mainly from osteoclasts) existed in the blood.
The determination of tracp-5b can reflect osteoclast activity.
CSB-E08492m is specifically for TRACP-5b, properly used for the mouse matrix samples.
The common samples are serum and plasma. The sample adding volume is 100ul. There is no need to dilute.
As for the TRAP in culture supernatants, pls arrange the preliminary experiment. Pls kindly note detects the mouse TRACP-5b not TRAP.
Any question, pls feel free to contact me.
Customer Reviews

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