I’m searching for ELISA kits to detect TRAP in mouse plasma/serum and in culture supernatants of osteoclasts cultured on bovine bone slices.
I saw that Cusabio proposes a kit for the detection of TRAP with the reference CSB-E08492m.
Can you tell me if this kit is specific of TRAP5b produced by the osteoclasts (no detection of TRAP produced by other cells)?
Also, do you know if 100 μL of serum/plasma is necessary for the test or if a dilution of the samples is required?
Finally, as I would like to measure TRAP in culture supernatants, I was wondering if conditioned media (alpha-MEM containing inactivated FBS and penicillin/streptomycin) can be used as samples and if bovine TRAP coming from bone slices or FBS will be detected by the kit.
Thank you in advance for your consideration.
Acp5 Proteins for Mus musculus (Mouse)
|Recombinant Mouse Tartrate-resistant acid phosphatase type 5(Acp5)|| Yeast
Detect Range: 20 μg/L-100 μg/L
Sensitivity: 5 μg/L
Detect Range: 1.25 μg/mL-20 μg/mL
Sensitivity: 1.25 μg/mL
Detect Range: 78.125 ng/mL-5000 ng/mL
Sensitivity: 69.748 ng/mL
Detect Range: 15.625 ng/mL-1000 ng/mL
Sensitivity: 13.797 ng/mL
Detect Range: 15.62 ng/mL-1000 ng/mL
Sensitivity: 3.9 ng/mL
Detect Range: 31.25 pg/mL-2000 pg/mL
Sensitivity: 0.002 pg/mL
Detect Range: 15.6 pg/mL-1000 pg/mL
Sensitivity: 3.9 pg/mL
Detect Range: 350 μg/mL-21.88 μg/mL
Sensitivity: 5.5 μg/mL