Rat Angiopoietin-related protein 4(ANGPTL4) ELISA kit

Code CSB-EL001712RA
Size 96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name angiopoietin-like 4
Alternative Names Angptl4Angiopoietin-related protein 4 ELISA kit; Angiopoietin-like protein 4 ELISA kit; Hepatic fibrinogen/angiopoietin-related protein ELISA kit; HFARP) [Cleaved into: ANGPTL4 N-terminal chain; ANGPTL4 C-terminal chain] ELISA kit
Abbreviation ANGPTL4
Uniprot No. Q6TMA8
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, bronchoalveolar lavage fluid, urine
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat ANGPTL4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:200 Average % 87  
Range % 83-102  
1:400 Average % 86  
Range % 81-90  
1:800 Average % 92  
Range % 87-98  
1:1600 Average % 102  
Range % 99-105  
Recovery
The recovery of rat ANGPTL4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 88-96  
EDTA plasma (n=4) 89 85-93  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
\\\"\\\"
pg/ml OD1 OD2 Average Corrected  
500 2.634 2.687 2.661 2.561  
250 2.226 2.268 2.247 2.147  
125 1.568 1.534 1.551 1.451  
62.5 0.968 0.943 0.956 0.856  
31.25 0.527 0.568 0.548 0.448  
15.62 0.398 0.377 0.388 0.288  
7.8 0.274 0.289 0.282 0.182  
0 0.099 0.101 0.100    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Mediates inactivation of the lipoprotein lipase LPL, and thereby plays a role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism. May also play a role in regulating glucose homeostasis and insulin sensitivity. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. Upon heterologous expression, inhibits the adhesion of endothelial cell to the extracellular matrix (ECM), and inhibits the reorganization of the actin cytoskeleton, formation of actin stress fibers and focal adhesions in endothelial cells that have adhered to ANGPTL4-containing ECM (in vitro). Depending on context, may modulate tumor-related angiogenesis.; Mediates inactivation of the lipoprotein lipase LPL, and thereby plays an important role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism. Has higher activity in LPL inactivation than the uncleaved protein.
Gene References into Functions
  1. this study represents the first investigation to demonstrate that Angptl4 can predict podocyte injury at earlier stages in minimal change disease PMID: 26352670
  2. GR and FoxO1 are required for Angptl4 transcription activation, and that FoxO1 negatively mediates the suppressive effect of insulin PMID: 24565756
  3. the overexpression of Angptl4 exerts protective, anti-inflammatory and anti-angiogenic effects. It re-presents a novel therapeutic target gene for the treatment of acute lung injury induced by LPS. PMID: 23783408
  4. Data suggest that endothelial lipoprotein lipase (LPL) levels in the heart are altered to maintain FA flux and may exploit angiopoietin-like protein-4 (ANGPTL4). PMID: 22226882
  5. Angptl4 is a novel methylation-silenced gene both in rat and human mammary carcinomas. PMID: 21489049
  6. Angptl-4 is a fasting-induced controller of lipoprotein lipase in adipose tissue, acting extracellularly on the native conformation in an unusual fashion, like an unfolding molecular chaperone PMID: 17088546
  7. ANGPTL4 is a direct GR target that participates in glucocorticoid-regulated triglyceride metabolism. PMID: 19628874

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Subcellular Location Secreted. Secreted, extracellular space, extracellular matrix.
Database Links

KEGG: rno:362850

STRING: 10116.ENSRNOP00000010031

UniGene: Rn.119611

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