Rat Ciliary Neurotrophic Factor,CNTF ELISA Kit

Code CSB-E04528r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
ciliary neurotrophic factor
Alternative Names
CntfCiliary neurotrophic factor ELISA kit; CNTF ELISA kit
Uniprot No.
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
3.9 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat CNTF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 95
Range % 92-100
1:2 Average % 90
Range % 82-95
1:4 Average % 103
Range % 99-110
1:8 Average % 87
Range % 82-98
The recovery of rat CNTF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 82-95
EDTA plasma (n=4) 108 106-113
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.121 1.985 2.053 1.913
500 1.598 1.543 1.571 1.431
250 1.223 1.144 1.184 1.044
125 0.865 0.911 0.888 0.748
62 0.542 0.513 0.528 0.388
31.2 0.339 0.331 0.335 0.195
15.6 0.257 0.255 0.256 0.116
0 0.142 0.137 0.140  
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Rat CNTF ELISA Kit was designed for the quantitative measurement of Rat CNTF protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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Target Background

(From Uniprot)
CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
Gene References into Functions
  1. endogenous SNCA plays a crucial role in spinal neuronal survival, in which the underlying mechanism may be linked to the regulation both apoptotic genes (Caspase7/9) and CNTF. PMID: 27581683
  2. Activation of CNTF/CNTFRalpha signaling pathway by hRheb(S16H) transduction of dopaminergic neurons PMID: 25799580
  3. FGF-2 down-regulated the expression of endogenous CNTF. PMID: 23271288
  4. CNTF induces a decrease in the sodium current and an increase in resting potential as in sodium inversion potential. PMID: 23665214
  5. Hence we can conclude that increase in the expression of ciliary neurotropic factor gene, as a nerve growth factor, following ultrasound radiation, can be considered as the reason of the effect of ultrasound on the rate of injured nerve regeneration. PMID: 23982826
  6. NO signaling induces CNTF expression in astrocytes that favors the beneficial outcomes of reactive astrogliosis in vivo. PMID: 23264628
  7. The data strongly suggested that intracellular ciliary neurotrophic factor may directly modulate proopiomelanocortin gene expression via the activation of ciliary neurotrophic factor receptors in the cell nucleus. PMID: 22146310
  8. The results of this study showed taht increased CNTF and CNTFR complex in the sprouting, metabolically active supraoptic nucleus are related directly to the sprouting response and not the increase in neurosecretory activity. PMID: 22037350
  9. CNTF promotes motor reinnervation of the MCN stump after its end-to-side neurorrhaphy with ulnar nerve and improves functional recovery of the biceps brachii muscle. PMID: 21696588
  10. Sympathetic neurons respond to stretch with an upregulation of VEGF, which is mediated by the NGF/CNTF and TrkA signaling pathway paralleled by HIF-1alpha expression. PMID: 21640078
  11. CNTF induces regeneration of cone outer segments in a rat model of retinal degeneration PMID: 20209167
  12. The CNTF mRNA in the distal stumps of injured spinal cords increased significantly on postoperation days 1-3, then returned to normal level on day 14. PMID: 19626993
  13. activates spinal cord astrocytes, stimulating their production and release of fibroblast growth factor-2, to increase motor neuron survival PMID: 11771938
  14. CNTF promotes the outgrowth of embryonic cranial motor neurons in isolated stage E12 hindbrain explants. PMID: 11932952
  15. CNTF is expressed in the rat pineal gland and eyes during embryonic development and supports the in vitro survival of neonatal sympathetic neurons, which innervate pineal glands immediately after birth. PMID: 11973480
  16. Retinoic acid had no significant effect on the expression of this protein in OLN-93 oligodendrocytes. PMID: 12397370
  17. CNTF affects adipocyte gene expression, and the specific receptor for this cytokine is induced in rodent models of obesity/type II diabetes PMID: 12424252
  18. In olfactory bulb, ciliary neurotrophic factor is localized to ensheathing cell nuclei, cell bodies and axon-enveloping processes; additionally, individual axons of olfactory neurons are CNTF immunoreactive. PMID: 12849744
  19. Our novel findings of increased cardiac CNTF and cardiopulmonary MCP-1 mRNA indicate a role for these factors in the pathogenesis of heart failure. PMID: 12950323
  20. Results show that ciliary neurotrophic factor (CNTF) and its receptor (CNTFRalpha) are differentially regulated in hippocampal neurons and reactive astrocytes following kainic acid injection. PMID: 15179044
  21. CNTF and its receptors are differentially expressed after optic nerve transection and could help delay retinal ganglion cell death in such a stressful environment. PMID: 15193523
  22. data suggest CNTF decreases lean body weight through a combination of appetite inhibition & ubiquitin-proteasome proteolytic pathway activation; findings show that CNTF is at least in part responsible for anorexia & weight loss found with neuronal injury PMID: 15304243
  23. CNTF-CNTFRalpha activates the JAK/STAT pathway leading to enhanced Cx43 expression and intercellular coupling PMID: 15342787
  24. CNTF/CPT-cAMP-induced retinal ganglion cell survival & axonal regeneration are a result of multiple pathway actions, with PKA as an essential component, but these pathways can function in an antagonistic manner under different conditions. PMID: 15574731
  25. CNTF expression is up-regulated from the day following intracerebral hemorrhage, with increased expression observed in brain tissue surrounding the hematoma lesion and white matter structures in association with astroglial proliferation. PMID: 15755520
  26. in skeletal muscle, CNTF can rapidly decrease sodium currents by altering inactivation gating, probably through an intracellular PKC-dependent mechanism that could lead to decreased membrane excitability PMID: 15831538
  27. Ciliary neurotrophic factor prevents acute lipid-induced insulin resistance by attenuating ceramide accumulation and phosphorylation of c-Jun N-terminal kinase in peripheral tissues PMID: 16396984
  28. A notable decrease in ciliary neurotrophic factor (CNTF) was demonstrated by Western blot after ammonia treatment, concurring with the reduction in CNTF mRNA observed in DNA microarrays. PMID: 16483693
  29. Ciliary neural trophic factor may mimic leptin's effects in skeletal muscle [comment] PMID: 16617151
  30. Results indicate that Sox10 is necessary and sufficient for regulating ciliary neurotrophic factor expression in the peripheral nervous system. PMID: 16684879
  31. Three-dimensional reconstructions of CNTF-immunoreactive axonal bulbar projections of these neurons revealed an ordered bilaterally symmetric pattern. PMID: 16999202
  32. The CNTF signaling pathway acts via regulation of nitric oxide production to modulate developmental programmed cell death of postmitotic rod precursor cells. PMID: 17054938
  33. Co-administration of ciliary neurotrophic factor with its soluble receptor protects against neuronal death and enhances neurite outgrowth. PMID: 18086669
  34. Cntf negatively regulates the phototransduction machinery in rod photoreceptors: implications for light-induced photostasis plasticity. PMID: 18188971
  35. Differentiation of TsAM5D chromaffin cells by glial cell derived neurotrophic factor (GDNF) plus CNTF at 39 degrees C become dependent solely on nerve growth factor (NGF) for their survival and neurite outgrowth. PMID: 18293415
  36. CNTF can increase microglial phagocytosis through a calcium-mediated pathway. Our results also suggest that the upregulation of alphav integrin by CNTF could be involved in the increased phagocytotic activity of microglia. PMID: 18401707
  37. Data show that both ciliary neurotrophic factor and fibroblast growth factor-2 are present in regions of elevated oligodendrocyte progenitor cell proliferation and generation after spinal cord injury and may play a role in injury-induced gliogenesis. PMID: 18615534
  38. increased CNTF occurs in response to conditions which induce high levels of phagocytic activity by perivascular cells in the axotomized neurohypophysis PMID: 18805412
  39. CNTF may act as a protective factor against weight gain during hypercaloric diet and could account for individual differences in the susceptibility to obesity. PMID: 18950628
  40. study showed CNTF is up-regulated by ammonia exposure, through mediation of p38 MAPK activation in astrocytes; also observed that SAPK/JNK & Erk1/2 activations in oligodendrocytes & neurons also play indirect roles in CNTF synthesis by astrocytes PMID: 18992343
  41. studies indicate that CNTF can activate microglia and dendritic-like microglia similar to interleukin-6 (IL-6); however, unlike IL-6, CNTF does not stimulate the expected signaling pathways in microglia, nor does it appear to require gp130 PMID: 19267906
  42. CNTF could promote axon regeneration, Schwann cell migration, monocyte infiltration and activation. CNTF might also indirectly promote axonal regeneration by further activating the JAK-STAT3 pathway. PMID: 19289286
  43. Data demonstrate that CNTF is a potent axon growth-promoting factor for mature retinal ganglion cells (RGCs), and that exogenously applied CNTF stimulates RGCs indirectly via a mechanism that depends on astrocyte-derived CNTF. PMID: 19332123
  44. The effect of gp130 stimulation on glutamate-induced excitotoxicity in primary hippocampal neurons PMID: 12150983

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Subcellular Location
Protein Families
CNTF family
Tissue Specificity
Nervous system.
Database Links
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