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24T ELISA Kit Trial Size (Only USD$150/ kit)
|Intra-assay Precision (Precision within an assay): CV%<8%
|Three samples of known concentration were tested twenty times on one plate to assess.
|Inter-assay Precision (Precision between assays): CV%<10%
|Three samples of known concentration were tested in twenty assays to assess.
|To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
|The recovery of rat IL-33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
|Average % Recovery
|EDTA plasma (n=4)
|These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
This rat IL33 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of rat IL33 in multiple samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of IL33 bound in the initial step.
IL33 is released upon tissue stress or damage to act as an alarmin. Different from other IL-1 family members such as IL18 and IL-1β, IL33 is inactivated after caspase cleavage. IL33 binds to its receptor IL-1RL1/ST2 and IL1RAP, activating downstream signaling molecules including MyD88 and TRAF6 ultimately inducing NF-κB, p38, JNK, and ERK pathways. IL33 primarily participates in the induction of type-2 immune responses, eliciting the release of Th2 cytokines thus facilitating allergic reactions. It also supports tissue repair by coordinating the action of innate lymphoid cells (ILCs) and regulatory T cells (Tregs). Additionally, IL33 acts in tissue homeostasis, growth, and immune response. It is associated with the pathogenesis of various diseases, including infection, inflammatory disorders, skin diseases, and various cancers.
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