Rat Interleukin 33(IL-33) ELISA Kit

Code CSB-E14077r
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This rat IL33 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of rat IL33 in multiple samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of IL33 bound in the initial step.

IL33 is released upon tissue stress or damage to act as an alarmin. Different from other IL-1 family members such as IL18 and IL-1β, IL33 is inactivated after caspase cleavage. IL33 binds to its receptor IL-1RL1/ST2 and IL1RAP, activating downstream signaling molecules including MyD88 and TRAF6 ultimately inducing NF-κB, p38, JNK, and ERK pathways. IL33 primarily participates in the induction of type-2 immune responses, eliciting the release of Th2 cytokines thus facilitating allergic reactions. It also supports tissue repair by coordinating the action of innate lymphoid cells (ILCs) and regulatory T cells (Tregs). Additionally, IL33 acts in tissue homeostasis, growth, and immune response. It is associated with the pathogenesis of various diseases, including infection, inflammatory disorders, skin diseases, and various cancers.

Target Name interleukin 33
Alternative Names Il33Interleukin-33 ELISA kit; IL-33 ELISA kit
Abbreviation IL33
Uniprot No. Q66H70
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 103  
Range % 98-108  
1:2 Average % 96  
Range % 91-101  
1:4 Average % 89  
Range % 83-95  
1:8 Average % 98  
Range % 93-104  
Recovery
The recovery of rat IL-33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 85-94  
EDTA plasma (n=4) 94 90-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.981 2.998 2.990 2.842  
500 1.967 1.873 1.920 1.772  
250 1.333 1.427 1.380 1.232  
125 0.845 0.835 0.840 0.692  
62.5 0.565 0.559 0.562 0.414  
31.2 0.331 0.348 0.340 0.192  
15.6 0.225 0.236 0.231 0.083  
0 0.143 0.153 0.148    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury.; In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation.
Gene References into Functions
  1. The release of IL-33 from necrotic cells might induce autophagy, which can further balance the effects of increased apoptosis secondary to contrast-induced nephropathy in diabetic kidney disease. PMID: 27055881
  2. TNF-alpha stimulated IL-33/sST2 and subsequent increase of IL-6 may be the mechanism of IL-33 in acute pancreatitis. PMID: 26927343
  3. poly-IC and IL-33 play a role in myelin repair by enhancing expression of myelin genes. PMID: 27022724
  4. IL-33 protects against myocardial I/R injury by inhibiting inflammatory responses and myocardial apoptosis. PMID: 26571038
  5. These findings indicate that IL-33 might play an important role in the inflammatory response following subarachnoid hemorrhage. PMID: 25417195
  6. MRAs play a pivotal role in the Gal-3 and IL-33/ST2 modulation in post-MI cardiac remodeling. PMID: 25458175
  7. Induced interleukin-33 expression enhances the tumorigenic activity of rat glioma cells. PMID: 24327583
  8. Data indicate IL-33 concentration was increased in the pancreas in duct ligation-induced acute pancreatitis. PMID: 23418608
  9. The effects of external application of IL1beta and IL33 on the cardiomyocyte transcriptome, were compared. PMID: 23219998
  10. This study examined the expression of IL-33 and the IL-33-mediated regulation of cell functions in pancreatic stellate cells isolated from human and rat pancreas tissues. PMID: 20689058

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Subcellular Location Nucleus. Chromosome. Cytoplasm. Cytoplasmic vesicle, secretory vesicle. Secreted.
Protein Families IL-1 family
Database Links

KEGG: rno:361749

UniGene: Rn.106849

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