Rat Osteopontin,OPN ELISA Kit

Instructions
Code CSB-E08393r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name secreted phosphoprotein 1
Alternative Names Spp1 ELISA Kit; 2b7 ELISA Kit; Spp-1 ELISA Kit; Osteopontin ELISA Kit; Bone sialoprotein 1 ELISA Kit; Secreted phosphoprotein 1 ELISA Kit; SPP-1 ELISA Kit
Abbreviation SPP1
Uniprot No. P08721
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat OPN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 88  
Range % 84-96  
1:200 Average % 96  
Range % 90-102  
1:400 Average % 84  
Range % 81-87  
1:800 Average % 88  
Range % 83-94  
Recovery
The recovery of rat OPN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 101 96-105  
EDTA plasma (n=4) 94 90-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.801 2.774 2.788 2.649  
1000 2.345 2.451 2.398 2.259  
500 1.625 1.635 1.630 1.491  
250 0.967 0.890 0.929 0.790  
125 0.548 0.532 0.540 0.401  
62.5 0.343 0.355 0.349 0.210  
31.25 0.161 0.148 0.155 0.016  
0 0.144 0.133 0.139    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Background

Function
(From Uniprot)
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.; FUNCTION
Gene References into Functions
  1. glomerular and tubular OPN mRNA expression was correlated with progressive renal damage PMID: 27717860
  2. These results demonstrate that OPN has an impact on the proliferation and differentiation of RASMCs stimulated by gingipains. PMID: 28302392
  3. Osteopontin plays a key role in vascular smooth muscle cell via signaling pathways involving AP-1 and C/EBP beta, leading to increases in vascular smooth muscle cell proliferation and subsequent vascular remodeling. PMID: 27089830
  4. Urinary osteopontin and neutrophil gelatinase associated lipocalin can be used in the detection of drug-induced acute kidney injury. PMID: 27026710
  5. Secreted phosphoprotein 1 is a key regulatory factor that affects nerve degeneration and regeneration through c-Fos, PKCalpha and p-ERK/ERK pathways after rat sciatic nerve injury. PMID: 28270094
  6. The extent of calcium oxalate crystal deposition is directly associated with the expression of osteopontin, inflammation and endoplasmic reticulum stress in renal tissue. PMID: 28442375
  7. rIPC exhibited protective effects against renal I/R injury which might be due to inhibition of OPN expression, inflammatory cytokine TGF-beta and caspase-3 and activation of anti-apoptotic protein survivin as well as improvement of oxidative stress in kidney tissues PMID: 27486809
  8. OPN is elevated in the aortas of diabetic rats. PMID: 27743892
  9. The analysis showed that the expression profiles of Spp1 signaling-related genes were notably different in four types of liver diseases: nonalcoholic fatty liver disease, liver cirrhosis, liver cancer, and acute liver failure. (Review) PMID: 27659347
  10. OPN might enhance inflammation and cell proliferation, attenuate cell apoptosis, and ultimately facilitate liver regeneration at the termination stage of liver regeneration. PMID: 27585571
  11. OPN induces oxidative stress via the involvement of mitochondria and NOX-4. It may affect mitochondrial morphology and integrity, at least in part, via the involvement of BIK. PMID: 27262843
  12. the genes expression changes of OPN signaling pathways may account for enhanced cell proliferation, survival, adhesion and migration, augmented inflammation response and attenuated apoptosis during liver regeneration. PMID: 26581507
  13. OPN plays an important role in the neuroprotection elicited by Hyperbaric oxygen preconditioning. PMID: 25146847
  14. OPN may directly upregulate VEGF expression through the PI3K/AKT and ERK1/2 pathway. PMID: 26099282
  15. In response to exposure to high concentration of calcium, renal tubular epithelial NRK cells increase the production of OPN, which may have a promoting role in CaOx crystal adhesion to NRK cells by calcium stimulation. PMID: 26216642
  16. It was found that BMP-6 and osteopontin considerably participate in progressive renal fibrosis through development of myofibroblasts. PMID: 25467747
  17. The expression of Spp1 in epididymal adipose tissue and liver was not modified by sleeve gastrectomy in obese mice. PMID: 24687376
  18. The expression of OPN mRNA was markedly inhibited in the renal stone formation model by concomitant administration of estradiol and/or progesterone. PMID: 24449951
  19. Osteopontin itself can induce EMT on NRK52E cells, and the process is associated with the upregulation of TGF-b1. PMID: 23788374
  20. Altered fetal expression of the osteopontin-CD44-integrin-receptor-system in the MWF mutant nephron deficit model points to a possible role in low nephron number hypertension and cardiovascular disease. PMID: 23241663
  21. H2O2 stimulates OPN expression in a unique biphasic pattern, where early increases are translational and late increases are transcriptional. PMID: 24247243
  22. miR181a regulates OPN expression and that altering miR181a expression may be a novel therapeutic approach to modulate OPN protein expression. PMID: 23466073
  23. Data suggest that expression of osteopontin in white adipose tissue can be regulated by dietary factors; osteopontin expression, which is up-regulated in diet-induced obesity, is down-regulated by reducing diet fat or replacing lard with soybean oil. PMID: 22847642
  24. Distribution patterns of immunoreactive OPN and osteocalcin (OC)-containing neurons are similar in many structures within the hindbrain. OC may play a role in modulating the neuroprotective function of OPN. PMID: 22552891
  25. OPN may play an important role in the pathogenesis of arterial restenosis via regulating its downstream genes. PMID: 23467880
  26. Reduction of OPN in vivo inhibited progression of chronic allograft nephropathy. PMID: 23498805
  27. phosphorylations are critical to OPN's ability to inhibit nucleation, whereas the growth of the hydroxyapatite crystals is effectively controlled by the highly acidic OPN polypeptide PMID: 23457612
  28. OPN bound to calcium deposits may lead to phagocytosis of debris and may participate in the regulation of ectopic calcification in the ischemic brain. PMID: 22087764
  29. findings indicate that injury-induced osteopontin may support neurite sprouting, suggesting a role for this molecule in recovery from central nervous system trauma PMID: 22692550
  30. VEGF/Flt-1 signaling plays a significant role in vascular inflammation and neointima formation by regulating osteopontin expression in vascular adevntitial fibroblasts. PMID: 22814749
  31. The data indicate an interaction between osteopontin(OPN)and calcium in the hippocampus in the chronic period after ischemia, suggesting that OPN binding to calcium deposits may be involved in scavenging mechanisms. PMID: 22496158
  32. Calcitonin gene-related peptide significantly upregulated Cnn1 and downregulated OPN in corpus cavernosum smooth muscle cells from diabetic rats with erectile dysfunction. PMID: 22049796
  33. Results indicate that osteopontin may play an important role of differentiation and osteoblasts and cementoblasts matrix formation during periodontal tissue remodeling during orthodontic tooth movement. PMID: 21806466
  34. Osteopontin is involved in the urotensin II-induced migration of adventitial fibroblasts, and UII could upregulate osteopontin gene expression and protein synthesis in fibroblasts. PMID: 22036853
  35. Data suggest that that upregulation of osteopontin expression in adventitial fibroblasts might be important in the pathogenesis of vascular remodeling after arterial injury. PMID: 21949681
  36. OPN activates NFATc1 and enhances osteoclast survival through a Ca(2+)-NFAT-dependent pathway. PMID: 21940634
  37. Osteopontin expression was increased in dorsal root ganglia following laminectomy and was a useful biomarker for scar formation. PMID: 20838370
  38. Osteopontin secreted by brain macrophages in this model of stroke may be involved in the phagocytosis of fragmented cell debris and possibly not in the phagocytosis of axonic or myelin debris. PMID: 21264948
  39. role for induced OPN in the response to I-R injury in the right cerebral cortex PMID: 21283687
  40. Hypertension can lead to high expression of arterial osteopontin. PMID: 20396353
  41. Results suggest potential roles of osteopontin and its main source, the senescent myofibroblasts, in glomerular fibrosis. PMID: 20714131
  42. Regulation of osteopontin in colorectal cancer cells. PMID: 20596651
  43. OPN may be necessary for the survival of TH positive cells in SN but through the RGD-containing peptide fragment may also have neuroprotective properties relevant to Parkinson's disease. PMID: 20626561
  44. increased smooth muscle cell protein levels following treatment with oxidized low-density lipoprotein PMID: 20211246
  45. In early stage of acute rejection of renal allograft in rats, the expressions of osteopontin, IL15, perforin and granzyme B are up-regulated. PMID: 20450731
  46. Expression of osteopontin increases dramatically in the livers during the development of nonalcoholic fatty liver fibrosis. PMID: 19032867
  47. This stusy demonstrated that the temporal and cellular patterns of osteopontin induction in reactive glial cells in this in vitro model suggesting that osteopontin has a multifunctional role in the pathogenesis of ischemic injury. PMID: 20407481
  48. Overexpression of Wnt genes in Brown Norway rats is critical in the maintenance of their ischemic resistance. Activation of the Wnt signaling cascade reduces mitochondrial cytochrome c release and caspase 3 activity through the action of osteopontin. PMID: 20392802
  49. the results of the current study show that Egr-1 binding to the OPN promoter is positively regulated by TGF-beta, and that the p38 MAPK, JNK, and ERK pathways are involved in OPN-mediated Egr-1 upregulation. PMID: 20417179
  50. A reactive oxygen species-initiated and OPN-mediated signalling pathway may play an important role in accelerated restenosis after percutaneous coronary intervention. PMID: 19076167

Show More

Hide All

Subcellular Location Secreted
Protein Families Osteopontin family
Database Links

KEGG: rno:25353

STRING: 10116.ENSRNOP00000062358

UniGene: Rn.8871

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1