Rat P-Selectin ELISA kit

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Rat P-Selectin ELISA kit

Citations (10) Protocol

Code	CSB-E07399r
Size	96T,5×96T,10×96T
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Citations (10)
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Description

The rat SELP ELISA Kit is engineered for accurate measurement of rat SELP levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the SELP content in the sample. The color intensity is positively correlated with SELP content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.

SELP is an adhesion molecule found on the membranes of α-granules in platelets and the Weibel-Palade bodies in endothelial cells. It binds to monocytes, neutrophils, T cells, and platelets, mediating the rolling of blood cells on the surface of the endothelium and initiating the attachment of leukocytes circulating in the blood to platelets, endothelial cells, and other leukocytes at injured and inflamed sites. Soluble SELP is thought to be a marker of endothelial dysfunction in hypercholesterolemic patients. SELP is also involved in many acute inflammatory conditions mediated by neutrophils. It may also participate in chronic inflammatory conditions such as atherosclerosis because it regulates monocyte and T-lymphocyte emigration from the bloodstream.

Target Name

selectin P (granule membrane protein 140kDa, antigen CD62)

Alternative Names

Selp; P-selectin; CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD antigen CD62P

Abbreviation

SELP

Uniprot No.

P98106

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

9.38 ng/mL-600 ng/mL

Sensitivity

2.34 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat P-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	85-99
1:2	Average %	92
	Range %	84-105
1:4	Average %	97
	Range %	92-103
1:8	Average %	94
	Range %	82-98

Recovery

The recovery of rat P-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	95-100
EDTA plasma (n=4)	95	92-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
600	2.755	2.812	2.784	2.616
300	2.217	2.236	2.227	2.059
150	1.545	1.524	1.535	1.367
75	1.051	0.979	1.015	0.847
37.5	0.587	0.622	0.605	0.437
18.75	0.456	0.438	0.447	0.279
9.38	0.288	0.279	0.284	0.116
0	0.173	0.162	0.168

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

DM-induced Hypermethylation of IR and IGF1R attenuates mast cell activation and airway responsiveness in rats D Fu,Journal of Cellular and Molecular Medicine,2020
Heptapeptide-based modification leading to enhancing the action of MTCA on activated platelets, P-selectin, GPIIb/IIIa Ze Li.et al,FUTURE MED CHEM,2018
Assessment of Thrombotic Risk in Atrial Fibrillation with Ultrasound Molecular Imaging of P-Selectin Jing Y.et al,Thromb Haemost,2018
ATIQCTPC: a nanomedicine capable of targeting tumor and blocking thrombosis in vivo Xu X.et al,Int J Nanomedicine.,2017
IQCA-TAVV: To explore the effect of P-selectin, GPIIb/IIIa, IL-2, IL-6 and IL-8 on deep venous thrombosis Wu J.et al,Oncotarget,2017
Protective role of B-patchoulene from Pogostemon cablin against indomethacin-induced gastric ulcer in rats: Involvement of anti-inflammation Jia-Zhen Wu.et al,Phytomedicine,2017
IQCA-TASS: a nano-scaled P-selectin inhibitor capable of targeting thrombus and releasing IQCA/TARGD (S) S in vivo Jianhui Wu.et al,Journal of Materials Chemistry B,2016
Aqueous extract of Rabdosia rubescens leaves: forming nanoparticles, targeting P-selectin, and inhibiting thrombosis Wang Y. et al,Int J Nanomedicine,2015
Impaired NO-mediated vasodilatation in rat coronary arteries after asphyxial cardiac arrest. Troelsen TT.et al,Acta Anaesthesiol Scand.,2015
The application of tetrahydroisoquinoline-3-carbonyl-TARGD (F) F as an anti-thrombotic agent having dual mechanisms of action Yang G et al,Mol Biosyst,2012

■ Customer Reviews

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■ Q&A

Hello,
please let me know whether this elisa kit can be use to detect the P selectin which is released from platelet or not.

Thanks for your inquiry.
Sorry we did not ever validate it before.
We suggest you do pre-testing first and determine it by yourself.

Function (From Uniprot)	Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG.
Gene References into Functions	Aortic cyclooxygenase-2 and P-selectin were significantly down-regulated in plant sterols and plant sterols + tripeptides groups. PMID: 22575043 Fibrinogen can interact with P-selectin in order to contribute to the development of atherosclerosis, high levels of fibrinogen and P-selectin can be regarded as risk factors for markers of atherosclerosis. PMID: 22340239 Renal injury after ischemia/reperfusion may relate to the strong expression of P-selectin. NO may have a protective affect by decreasing the expression of P-selectin and alleviating the adhesion, aggregation and infiltration of neutrophils. PMID: 21180132 Both baicalin and octreotide can exert some protective effects on multiple organs of rats with severe acute pancreatitis via decreased P-selectin expression. PMID: 19780886 Volatile oil of Magnolia biondii can protect the kidney in rats with diabetic nephropathy by inhibiting the expressions of P-selectin protein in serum and in renal tissue. PMID: 18471420 P-selectin, contribute to massive infiltration of Mo into the interstitium by functioning as specialized post-capillary venules. PMID: 12081568 P-selectin was highly expressed on glomerular endothelial cells after injection of nephrotoxic serum.P-selectin partially mediates glomerular infiltration of macrophage in experimental crescentic glomerulonephritis. PMID: 12100021 Blockade of this molecule protects rat steatotic liver grafts from ischemia/reperfusion injury PMID: 12201360 P-selectin increases CD45-positive leukocyte activation, inflammation and neointimal formation in a Zucker diabetic rat carotid artery balloon injury model. PMID: 12377736 Neointimal rat SMCs supported a 2.5-fold higher arrest of monocytes and "memory" T lymphocytes than medial SMCs, which was dependent on both P-selectin and VLA-4. PMID: 14963004 P-selectin increased significantly in hepatic sinusoidal endothelial cells and renal tubular epithelial cells 1 h after ischemia-reperfusion PMID: 15742404 expression of the P-selectin gene is induced through the activation of NF-kappaB and that P-selectin may be involved in the pathogenesis of diabetic nephropathy PMID: 15888973 P-selectin plays a role in ischemia-reperfusion injury in experimental myocardial infarct. PMID: 17488661 Show More Hide All
Subcellular Location	Cell membrane; Single-pass type I membrane protein.
Protein Families	Selectin/LECAM family
Tissue Specificity	Not detected in the absence of exposure to lipopolysaccharide (LPS). Detected only after exposure to lipopolysaccharide (LPS) in the tissues examined: spleen, lung, brain, liver, heart, kidney, thymus and small intestine.
Database Links	KEGG: rno:25651 STRING: 10116.ENSRNOP00000003759 UniGene: Rn.10012

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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