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| Code | CSB-E07399r |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat P-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 85-99 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 84-105 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 82-98 | |||||
| The recovery of rat P-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 95-100 | ||||
| EDTA plasma (n=4) | 95 | 92-100 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The Rat P-Selectin ELISA kit is a powerful tool for researchers in the field of cardiovascular research. This ELISA kit allows for the quantitative measurement of P-selectin levels in a range of sample types from Rattus norvegicus (Rat), including serum, plasma, and tissue homogenates.
With a detection range of 9.38 ng/mL to 600 ng/mL and a sensitivity of 2.34 ng/mL, this ELISA kit delivers accurate and precise results, making it an essential tool for inflammation, immunology and cardiovascular research. The assay time is only 1-5 hours, and the sample volume required is only 50-100ul, making it a convenient and easy-to-use product.
The Rat P-Selectin ELISA kit utilizes a sandwich assay principle, where the P-selectin antigen is captured by a specific antibody and detected by a second antibody labeled with a detection reagent. The detection wavelength is 450 nm, providing a reliable and consistent readout.
This ELISA kit has been cited in over 10 publications, highlighting its reliability and utility in inflammation, immunology and cardiovascular research. It is a valuable addition to any research laboratory studying inflammation, immunology and cardiovascular research, providing accurate and reliable measurement of P-selectin levels in rat samples.
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Hello,
please let me know whether this elisa kit can be use to detect the P selectin which is released from platelet or not.
