Rat P-Selectin ELISA kit

Code CSB-E07399r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
selectin P (granule membrane protein 140kDa, antigen CD62)
Alternative Names
Selp; P-selectin; CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD antigen CD62P
Uniprot No.
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
9.38 ng/mL-600 ng/mL
2.34 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of rat P-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 85-99  
1:2 Average % 92  
Range % 84-105  
1:4 Average % 97  
Range % 92-103  
1:8 Average % 94  
Range % 82-98  
The recovery of rat P-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 95-100  
EDTA plasma (n=4) 95 92-100  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
600 2.755 2.812 2.784 2.616  
300 2.217 2.236 2.227 2.059  
150 1.545 1.524 1.535 1.367  
75 1.051 0.979 1.015 0.847  
37.5 0.587 0.622 0.605 0.437  
18.75 0.456 0.438 0.447 0.279  
9.38 0.288 0.279 0.284 0.116  
0 0.173 0.162 0.168    
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

The Rat P-Selectin ELISA kit is a powerful tool for researchers in the field of cardiovascular research. This ELISA kit allows for the quantitative measurement of P-selectin levels in a range of sample types from Rattus norvegicus (Rat), including serum, plasma, and tissue homogenates.

With a detection range of 9.38 ng/mL to 600 ng/mL and a sensitivity of 2.34 ng/mL, this ELISA kit delivers accurate and precise results, making it an essential tool for inflammation, immunology and cardiovascular research. The assay time is only 1-5 hours, and the sample volume required is only 50-100ul, making it a convenient and easy-to-use product.

The Rat P-Selectin ELISA kit utilizes a sandwich assay principle, where the P-selectin antigen is captured by a specific antibody and detected by a second antibody labeled with a detection reagent. The detection wavelength is 450 nm, providing a reliable and consistent readout.

This ELISA kit has been cited in over 10 publications, highlighting its reliability and utility in inflammation, immunology and cardiovascular research. It is a valuable addition to any research laboratory studying inflammation, immunology and cardiovascular research, providing accurate and reliable measurement of P-selectin levels in rat samples.


Customer Reviews and Q&A

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please let me know whether this elisa kit can be use to detect the P selectin which is released from platelet or not.

Thanks for your inquiry.
Sorry we did not ever validate it before.
We suggest you do pre-testing first and determine it by yourself.

Target Background

(From Uniprot)
Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG.
Gene References into Functions
  1. Aortic cyclooxygenase-2 and P-selectin were significantly down-regulated in plant sterols and plant sterols + tripeptides groups. PMID: 22575043
  2. Fibrinogen can interact with P-selectin in order to contribute to the development of atherosclerosis, high levels of fibrinogen and P-selectin can be regarded as risk factors for markers of atherosclerosis. PMID: 22340239
  3. Renal injury after ischemia/reperfusion may relate to the strong expression of P-selectin. NO may have a protective affect by decreasing the expression of P-selectin and alleviating the adhesion, aggregation and infiltration of neutrophils. PMID: 21180132
  4. Both baicalin and octreotide can exert some protective effects on multiple organs of rats with severe acute pancreatitis via decreased P-selectin expression. PMID: 19780886
  5. Volatile oil of Magnolia biondii can protect the kidney in rats with diabetic nephropathy by inhibiting the expressions of P-selectin protein in serum and in renal tissue. PMID: 18471420
  6. P-selectin, contribute to massive infiltration of Mo into the interstitium by functioning as specialized post-capillary venules. PMID: 12081568
  7. P-selectin was highly expressed on glomerular endothelial cells after injection of nephrotoxic serum.P-selectin partially mediates glomerular infiltration of macrophage in experimental crescentic glomerulonephritis. PMID: 12100021
  8. Blockade of this molecule protects rat steatotic liver grafts from ischemia/reperfusion injury PMID: 12201360
  9. P-selectin increases CD45-positive leukocyte activation, inflammation and neointimal formation in a Zucker diabetic rat carotid artery balloon injury model. PMID: 12377736
  10. Neointimal rat SMCs supported a 2.5-fold higher arrest of monocytes and "memory" T lymphocytes than medial SMCs, which was dependent on both P-selectin and VLA-4. PMID: 14963004
  11. P-selectin increased significantly in hepatic sinusoidal endothelial cells and renal tubular epithelial cells 1 h after ischemia-reperfusion PMID: 15742404
  12. expression of the P-selectin gene is induced through the activation of NF-kappaB and that P-selectin may be involved in the pathogenesis of diabetic nephropathy PMID: 15888973
  13. P-selectin plays a role in ischemia-reperfusion injury in experimental myocardial infarct. PMID: 17488661

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Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Protein Families
Selectin/LECAM family
Tissue Specificity
Not detected in the absence of exposure to lipopolysaccharide (LPS). Detected only after exposure to lipopolysaccharide (LPS) in the tissues examined: spleen, lung, brain, liver, heart, kidney, thymus and small intestine.
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