Code | CSB-E12632r |
Size | 96T,5×96T,10×96T |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of rat SP-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:5 | Average % | 88 | ||||
Range % | 82-92 | |||||
1:10 | Average % | 94 | ||||
Range % | 90-99 | |||||
1:20 | Average % | 95 | ||||
Range % | 91-101 | |||||
1:40 | Average % | 94 | ||||
Range % | 90-98 |
The recovery of rat SP-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 89 | 85-96 | ||||
EDTA plasma (n=4) | 92 | 87-98 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The Rat Pulmonary Surfactant-associated protein D (SP-D) ELISA kit can be your reliable and efficient solution for measuring the levels of Pulmonary surfactant-associated protein D in serum, plasma, and tissue homogenates from Rattus norvegicus (Rat) samples.
With a detection range of 0.312 ng/mL-20 ng/mL and a sensitivity of 0.078 ng/mL, this ELISA kit provides accurate and precise measurements of SP-D levels. The assay time ranges from 1-5 hours and requires a sample volume of 50-100ul, making it convenient and easy to use in your lab.
The assay principle is quantitative and the measurement method is a Sandwich assay, ensuring reliable and reproducible results. The detection wavelength is 450 nm.
Designed for research in Lung-related disease and Signal Transduction, this ELISA kit has been cited in more than 3 publications, attesting to its quality and reliability.
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