Rat nuclear factor-κB p65,NF-κB p65 ELISA Kit

Code CSB-E08788r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Rat NFκB p65 ELISA Kit was designed for the quantitative measurement of Rat NFκB p65 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL .

Target Name nuclear factor-κB p65,NF-κB p65
Abbreviation NFκB p65
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat NF-κB p65 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:100Average %89
Range %86-92
1:200Average %99
Range %97-103
1:400Average %89
Range %85-93
1:800Average %95
Range %91-99
The recovery of rat NF-κB p65 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9591-97
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
1002.039 2.022 2.031 1.887
501.334 1.326 1.330 1.186
250.908 0.902 0.905 0.761
12.50.566 0.557 0.562 0.418
6.250.358 0.364 0.361 0.217
3.120.291 0.281 0.286 0.142
1.560.243 0.232 0.238 0.094
00.145 0.143 0.144  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


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