| Description |
This Rat NFκB p65 ELISA Kit was designed for the quantitative measurement of Rat NFκB p65 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL . |
| Target Name |
nuclear factor-κB p65,NF-κB p65 |
| Abbreviation |
NFκB p65 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
1.56 pg/mL-100 pg/mL |
| Sensitivity |
0.39 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others
|
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | | Three samples of known concentration were tested twenty times on one plate to assess. | | | Inter-assay Precision (Precision between assays): CV%<10% | | | | | Three samples of known concentration were tested in twenty assays to assess. | | | | | | | | | | |
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat NF-κB p65 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | | | Sample | Serum(n=4) | | | 1:100 | Average % | 89 | | | Range % | 86-92 | | | 1:200 | Average % | 99 | | | Range % | 97-103 | | | 1:400 | Average % | 89 | | | Range % | 85-93 | | | 1:800 | Average % | 95 | | | Range % | 91-99 | |
|
| Recovery |
| The recovery of rat NF-κB p65 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
| Sample Type | Average % Recovery | Range | | | Serum (n=5) | 95 | 91-97 | | | EDTA plasma (n=4) | 94 | 90-98 | | | | | | | | | | | | | | | | | |
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 100 | 2.039 | 2.022 | 2.031 | 1.887 | | | 50 | 1.334 | 1.326 | 1.330 | 1.186 | | | 25 | 0.908 | 0.902 | 0.905 | 0.761 | | | 12.5 | 0.566 | 0.557 | 0.562 | 0.418 | | | 6.25 | 0.358 | 0.364 | 0.361 | 0.217 | | | 3.12 | 0.291 | 0.281 | 0.286 | 0.142 | | | 1.56 | 0.243 | 0.232 | 0.238 | 0.094 | | | 0 | 0.145 | 0.143 | 0.144 | | |
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| ELISA Data Analysis |
Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
