Rat procollagen Ⅰ N-terminal peptide,PⅠNP ELISA Kit

Rat procollagen Ⅰ N-terminal peptide,PⅠNP ELISA Kit

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Rat procollagen Ⅰ N-terminal peptide,PⅠNP ELISA Kit

Citations (13) Protocol

Code	CSB-E12774r
Size	96T,5×96T,10×96T
Trial Size
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Product Details
Citations (13)
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Description

This Rat PⅠNP ELISA Kit was designed for the quantitative measurement of Rat PⅠNP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL .

Target Name

procollagen Ⅰ N-terminal peptide,PⅠNP

Alternative Names

procollagen 1 N-terminal peptide,P1NP, procollagen I N-terminal peptide,PINP

Abbreviation

PⅠNP

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

62.5 pg/mL-4000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat PⅠNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	100
	Range %	95-105
1:10	Average %	96
	Range %	90-102
1:20	Average %	97
	Range %	92-108
1:40	Average %	96
	Range %	90-103

Recovery

The recovery of rat PⅠNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-103
EDTA plasma (n=4)	100	95-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.488	2.527	2.508	2.331
2000	1.848	1.839	1.844	1.667
1000	1.331	1.356	1.344	1.167
500	0.909	0.963	0.936	0.759
250	0.524	0.534	0.529	0.352
125	0.314	0.329	0.322	0.145
62.5	0.254	0.267	0.261	0.084
0	0.176	0.178	0.177

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Resistant dextrin protects against pathological bone loss in ovariectomized rats and inhibits RANKL-induced osteoclastogenesis H Wang,Histology and histopathology,2022
Metabolomics and physiological analysis of the effect of calcium supplements on reducing bone loss in ovariectomized rats by increasing estradiol levels H Mao,Nutrition & metabolism,2021
Fructus Ligustri Lucidi aqueous extract promotes calcium balance and short-chain fatty acids production in ovariectomized rats B Chen,Journal of Ethnopharmacology,2021
Vaspin antagonizes high fat-induced bone loss in rats and promotes osteoblastic differentiation in primary rat osteoblasts through Smad-Runx2 signaling Wang H,Nutrition & Metabolism,2020
Differential skeletal response in adult and aged rats to independent and combinatorial stimulation with pulsed electromagnetic fields and mechanical vibration Cai J,FASEB J,2020
Flaxseed oil ameliorated high-fat-diet-induced bone loss in rats by promoting osteoblastic function in rat primary osteoblasts Chen F, et al,Nutrition & Metabolism,2019
Glucagon like peptide 2 has a positive impact on osteoporosis in ovariectomized rats Xu B, et al,Life Sciences,2019
Strontium-loaded titania nanotube arrays repress osteoclast differentiation through multiple signalling pathways: In vitro and in vivo studies Mi B.et al,Sci Rep,2017
Promotion effect of extracts from plastrum testudinis on alendronate against glucocorticoid-induced osteoporosis in rat spine Hui Ren.et al,Sci Rep,2017
Local application of an ibandronate/collagen sponge improves femoral fracture healing in ovariectomized rats Guo J.et al,PLoS One,2017
Low-molecular weight fucoidan inhibits the differentiation of osteoclasts and reduces osteoporosis in ovariectomized rats Xin Jin.et al,Molecular Medicine Reports,2016
Variance of spinal osteoporosis induced by dexamethasone and methylprednisolone and its associated mechanism Ren H. et al,Steroids,2015
Effect of whole body vibration therapy on circulating serotonin levels in an ovariectomized rat model of osteoporosis Wei QS et al,Iran J Basic Med Sci,2014

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.