Name: Recombinant Human 5-hydroxytryptamine receptor 1B(HTR1B) (Active)
Brand: CUSABIO
SKU: CSB-CF010882HUb2

Product Details

Purity	Greater than 90% as determined by SDS-PAGE.
Activity	Measured by its binding ability in a functional ELISA. Immobilized HTR1B at 5 μg/ml can bind human GSTK1, the EC50 of human GSTK1 protein is 159.40-218.50 ng/ml.
Target Names	HTR1B
Uniprot No.	P28222
Research Area	Neuroscience
Alternative Names	5 HT1DB; 5 hydroxytryptamine receptor 1B; 5-HT-1B; 5-HT-1D-beta; 5-HT1B; 5-hydroxytryptamine receptor 1B; 5HT1B_HUMAN; Htr1b; HTR1D2; HTR1DB; S12; Serotonin 1D beta receptor; Serotonin receptor 1B
Species	Homo sapiens (Human)
Source	in vitro E.coli expression system
Expression Region	1-390aa
Target Protein Sequence	MEEPGAQCAPPPPAGSETWVPQANLSSAPSQNCSAKDYIYQDSISLPWKVLLVMLLALITLATTLSNAFVIATVYRTRKLHTPANYLIASLAVTDLLVSILVMPISTMYTVTGRWTLGQVVCDFWLSSDITCCTASILHLCVIALDRYWAITDAVEYSAKRTPKRAAVMIALVWVFSISISLPPFFWRQAKAEEEVSECVVNTDHILYTVYSTVGAFYFPTLLLIALYGRIYVEARSRILKQTPNRTGKRLTRAQLITDSPGSTSSVTSINSRVPDVPSESGSPVYVNQVKVRVSDALLEKKKLMAARERKATKTLGIILGAFIVCWLPFFIISLVMPICKDACWFHLAIFDFFTWLGYLNSLINPIIYTMSNEDFKQAFHKLIRFKCTS
Mol. Weight	62.1kDa
Protein Length	Full Length
Tag Info	N-terminal 10xHis-SUMO-tagged
Form	Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs	Protein FAQs
Storage Condition	Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time	Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA	Please contact us to get it.

Target Background

Function (From Uniprot)	G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for ergot alkaloid derivatives, various anxiolytic and antidepressant drugs and other psychoactive substances, such as lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Regulates the release of 5-hydroxytryptamine, dopamine and acetylcholine in the brain, and thereby affects neural activity, nociceptive processing, pain perception, mood and behavior. Besides, plays a role in vasoconstriction of cerebral arteries.
Gene References into Functions	Involvement of HTR1B variants in ADHD susceptibility as well as in externalizing and internalizing comorbidities, considering sex differences. PMID: 28923721 The polymorphism of HTR1B C861G is not a risk factor for Tourette's in Han Chinese population. PMID: 26123080 Acute tryptophan depletion (ATD) did not significantly influence participants' sensitivity to probability, wins, or losses, although there was a trend for a lower tendency to choose experimental gambles overall following depletion. Following ATD, participants with higher peripheral 5-HT1B transcription levels increased choice of experimental gambles and used information pertaining to possible wins more when making choices PMID: 27638901 This study found several promoter variants in linkage disequilibrium that may potentially influence the allelic imbalance in the C861G variant. aAnd no evidence of allelic imbalance nor parent-of-origin effects of the C861G variant was observed in suicidal behavior. PMID: 28445878 Single nucleotide polymorphism in HTR1B gene is associated with Attention deficit hyperactivity disorder. PMID: 28512748 nine ADHD candidate single nucleotide polymorphisms (SNPs) in seven genes were tested for association with PD in 5333 cases and 12,019 healthy controls. No significant association was observed. PMID: 28176268 results highlight the importance of HTR1 in leukemogenesis and LSC survival and identify this receptor family as a new target for therapy in AML with prognostic value PMID: 28193998 HTR polymorphisms related to vulnerability to major depressive disorder (MDD) and MDD with suicidal attempts. PMID: 28007644 The results of this study give new evidence for the role of epigenetic and genetic factors which could modulate HTR1B expression in the pharmacological response to antidepressants. PMID: 28025020 5-HT1B receptor-dependent cellular Src-related kinase-Nox1-pathways contribute to vascular remodeling in pulmonary arterial hypertension. PMID: 28473438 preliminary findings suggest a role of the HTR1B gene in susceptibility to development of bulimia nervosa subtypes. Furthermore, this gene might have an impact on the severity of anxiety in anorexia nervosa-spectrum patients. PMID: 27579596 Polymorphic variants of the HTR1B gene are associated with the susceptibility of secondary RP in vibration-exposed occupational populations of Chinese Han people. PMID: 26639766 large scale neural activation differences in the inferior and medial frontal and temporal/parietal regions of the response inhibition network between the different variants of both the HTR1B and 5HTT genes; activation in these regions was significantly associated with stop-task performance, but not with ADHD diagnosis or severity PMID: 26428549 Results from Parkinson's disease patients and MPTP-lesioned monkeys argue in favor of a contribution of 5-HT1B receptors in the pathophysiology of levodopa-induced motor complications PMID: 26254863 The interaction between two major serotonergic structures involved in serotonin release, the SERT and 5-HT1B receptor, results in a modification of the inhibitory serotonergic tone mediated via 5-HT1A receptors. PMID: 25652393 Serotonin 1B Receptor Gene (HTR1B) Methylation as a Risk Factor for Callous-Unemotional Traits in Antisocial Boys PMID: 25993020 Down-regulation of 5-HT1B and 5-HT1D receptors inhibits proliferation, clonogenicity and invasion of human pancreatic cancer cells. PMID: 25268648 reductions in 5-HT1B availability found in this study suggest that 5-HT1B receptors may contribute to the etiology or expression of cocaine dependence and potentially represent a target for medication development. PMID: 24433854 5-HT1B- and 5-HT1D-mediated signaling play an important role in the regulation of the proliferative and invasive phenotype of pancreatic cancer cells. PMID: 25170871 Genetic variation in HTR1B predicts changes in bone metabolism during SSRI use. PMID: 24074042 HTR1B SNPs significantly associate with early-onset obsessive compulsive disorder. PMID: 24571444 Low level of 5-HT(1B)receptors in limbic system corelates to decreased serotonin function in patients with Parkinson disease.(review) PMID: 24126162 HTR1B SNP rs6296 was associated with childhood aggressive behavior but not with adulthood anger or hostility. The HTR1B SNP rs6296 modified the association between childhood aggressive behavior and adulthood hostility. PMID: 22945537 5-HT inhibits pulmonary artery smooth muscle cell apoptosis via pERK1/2, PDK, 5-HT1B, 5-HTT pathway. PMID: 23601559 Alterations in 5HT1B-receptor density were found to be differentially linked to a novel, five-factor model of posttraumatic stress disorder symptoms. PMID: 22584868 An association between the sexual dimension of aging males' syndrome and genetic variants of 5-HTR1B G861C is observed. PMID: 23485949 Brain 5-HT1B receptor occupancy with zolmitriptan is demonstrated. PMID: 23430984 In a Turkish population of migraine patients no genetic association with 5-HT1B polymorphisms is found. PMID: 23375453 These results cannot provide support for an important function of TPH1 and HTR1B in the pathogenesis of sclerosing bone dysplasias. PMID: 23563356 This meta-analysis supports the associations of HTR1B -261T>G and -161A>T with alcohol and drug abuse and further investigations are warranted in larger samples. PMID: 23335468 The results of this study concluded that SSRIs reduce attention in older adults, particularly in those with high-expression genetic variants at the 1B receptors. PMID: 22717018 HTR1B genotypes AA & AT are associated with a lower age of onset of smoking in women than the TT genotype. PMID: 23177301 crystal structures of the 5-HT1B G protein-coupled receptor bound to the agonist antimigraine medications ergotamine and dihydroergotamine PMID: 23519210 Epistatic interactions between presynaptic components (5HTR1B and SLC6A4) as reported are likely to regulate serotonin levels in the synapse and impact treatment for ADHD. PMID: 22575343 The CC genotype of HTR1B G681C polymorphism had higher cortisol and prolactin responses and higher platelet-rich plasma serotonin in health volunteers but this was not observed in nonresponsive OCD with the same genotype after chronic SRI treatment. PMID: 22522758 age is a relevant factor for 5-HT(1B) in the cortex of healthy adults PMID: 22851636 Preliminary data imply that the genetic variants of the 5-HT1B receptor might be associated with prostate cancer PMID: 22420486 Neither the 5-HTTLPR gene nor the 5-HT1B G861C polymorphism alone is a risk factor for antisocial alcoholism in Taiwan's Han Chinese population, but that the interaction between both genes may increase susceptibility to antisocial alcoholism. PMID: 22550993 our findings point to an association between functional variants in the promoter region of the HTR1B gene and alcohol dependence, supporting previous neurobiological evidences of the involvement of HTR1B variations in alcohol-related phenotypes. PMID: 22005095 two SNPs, both serotonin receptor 5-HT(1B) linked, had a significant main effect on ejaculation latency time. PMID: 22240001 Selective inhibition of 5-HT2A by sarpogrelate stimulated angiogenesis, restoring ischemic limb blood perfusion in a severe diabetic mouse model through stimulation of the eNOS/Akt pathway via the endothelial 5-HT1B receptor. PMID: 22172591 There was a statistically significant association with Body Mass Index at HTR1B single nucleotide polymorphism rs4140535 in African Americans but not in Caucasians. PMID: 22106445 genetic association studies in Ireland/Northern Ireland: SNPs in HTR1B and OPRmu1 genes are associated with subgroups of subjects with alcohol dependence distinguished by age of onset. PMID: 21906503 These findings point to a role for HTR(1B) polymorphism in heroin dependence among Han Chinese, and may be informative for future genetic or neurobiological studies on heroin dependence. PMID: 21839728 Sumatriptan and tegaserod were more potent at the 124Cys-variant h5-HT1B receptor PMID: 21478802 findings suggest that allelic variability in SLC1A2/3, 5-HTR1B and NTRK2 may be relevant to the underlying diathesis for suicidal acts. PMID: 21711518 The HTR1B A-161T polymorphism may be particularly valuable as a functional genetic marker for alcoholis PMID: 21172311 our findings suggest abnormally reduced function of ventral striatum/ventral pallidum 5-HT1B receptors in humans with major depressive disorder PMID: 20480149 This study found haplotype of HTR1B gene that was significantly more frequent in worman then in mam. PMID: 20674037 There was no assocition of HTR1B with adolescent idiopathic scoliosis. PMID: 20388441 Show More Hide All
Subcellular Location	Cell membrane, Multi-pass membrane protein
Protein Families	G-protein coupled receptor 1 family
Tissue Specificity	Detected in cerebral artery smooth muscle cells (at protein level). Detected in brain cortex, striatum, amygdala, medulla, hippocampus, caudate nucleus and putamen.
Database Links	HGNC: 5287 OMIM: 182131 KEGG: hsa:3351 STRING: 9606.ENSP00000358963 UniGene: Hs.123016

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Code	CSB-CF010882HUb2
Size	US$3528Purchase it in Cusabio online store (only available for customers from the US)
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized HTR1B at 5 μg/ml can bind human GSTK1, the EC₅₀ of human GSTK1 protein is 159.40-218.50 ng/ml.
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Recombinant Human 5-hydroxytryptamine receptor 1B(HTR1B) (Active)

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