Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 ng/ml, corresponding to a specific activity of >1.0x105 IU/mg.
Research Area
Cell Biology
Alternative Names
SML 1; SML1; SP; Spasmolysin; Spasmolytic polypeptide; spasmolytic protein 1 ; TFF 2; TFF2; TFF2_HUMAN; trefoil factor 2 (spasmolytic protein 1) ; Trefoil factor 2; Trefoil factor 2 precursor
Species
Homo sapiens (Human)
Expression Region
24-129aa
Complete Sequence
EKPSPCQCSR LSPHNRTNCG FPGITSDQCF DNGCCFDSSV TGVPWCFHPL PKQESDQCVM EVSDRRNCGY PGISPEECAS RKCCFSNFIF EVPWCFFPKS VEDCHY
Protein Length
Full Length of Mature Protein
Buffer
Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 130mM NaCl
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
5-10 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Recombinant Human TFF2 is a top-quality, tag-free full-length mature protein that corresponds to the 24-129aa expression region of Trefoil factor 2 (Spasmolysin). Designed specifically for use in cell biology research, this protein is expressed in E. coli and boasts a purity of over 97% as determined by SDS-PAGE and HPLC. Additionally, its endotoxin levels are less than 1.0 EU/µg, as measured by the LAL method, ensuring minimal interference in your experiments.
Recombinant Human TFF2 is fully biologically active, with an ED50, as determined by a chemotaxis bioassay using human MCF-7 cells, of less than 10 ng/mL. This corresponds to a specific activity of > 1.0 × 105 IU/mg, showcasing the protein's effectiveness in various research applications. Supplied in a lyophilized powder form, this product offers convenient storage and use across a wide range of experiments.