Product Details

Purity

>97% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 ng/ml, corresponding to a specific activity of >1.0x10⁵ IU/mg.

Target Names

TFF2

Uniprot No.

Q03403

Research Area

Cell Biology

Alternative Names

SML 1; SML1; SP; Spasmolysin; Spasmolytic polypeptide; spasmolytic protein 1 ; TFF 2; TFF2; TFF2_HUMAN; trefoil factor 2 (spasmolytic protein 1) ; Trefoil factor 2; Trefoil factor 2 precursor

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

24-129aa

Complete Sequence

EKPSPCQCSR LSPHNRTNCG FPGITSDQCF DNGCCFDSSV TGVPWCFHPL PKQESDQCVM EVSDRRNCGY PGISPEECAS RKCCFSNFIF EVPWCFFPKS VEDCHY

Mol. Weight

12.0 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 130mM NaCl

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Recombinant Human TFF2 is a top-quality, tag-free full-length mature protein that corresponds to the 24-129aa expression region of Trefoil factor 2 (Spasmolysin). Designed specifically for use in cell biology research, this protein is expressed in E. coli and boasts a purity of over 97% as determined by SDS-PAGE and HPLC. Additionally, its endotoxin levels are less than 1.0 EU/µg, as measured by the LAL method, ensuring minimal interference in your experiments.

Recombinant Human TFF2 is fully biologically active, with an ED₅₀, as determined by a chemotaxis bioassay using human MCF-7 cells, of less than 10 ng/mL. This corresponds to a specific activity of ＞ 1.0 × 105 IU/mg, showcasing the protein's effectiveness in various research applications. Supplied in a lyophilized powder form, this product offers convenient storage and use across a wide range of experiments.

Code	CSB-AP000351HU
Abbreviation	Recombinant Human TFF2 protein (Active)
MSDS	MSDS Datasheet
Size	$142
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Recombinant Human Trefoil factor 2 protein (TFF2) (Active)

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