Recombinant Human Trefoil factor 2 protein(TFF2) (Active)

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Code CSB-AP000351HU
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Product Details

Purity >97% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 ng/ml, corresponding to a specific activity of >1.0x105 IU/mg.
Target Names TFF2
Uniprot No. Q03403
Research Area Cell Biology
Alternative Names SML 1; SML1; SP; Spasmolysin; Spasmolytic polypeptide; spasmolytic protein 1 ; TFF 2; TFF2; TFF2_HUMAN; trefoil factor 2 (spasmolytic protein 1) ; Trefoil factor 2; Trefoil factor 2 precursor
Species Homo sapiens (Human)
Source E.coli
Expression Region 24-129aa
Mol. Weight 12.0 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 130mM NaCl
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 5-10 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

(From Uniprot)
Inhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains (By similarity).
Gene References into Functions
  1. Data show that trefoil factor 2 (TFF2( urine levels continuously decreased with disease progression, TFF2 serum concentrations progressively increased from the early to later chronic kidney disease (CKD) stages, indicating changes in renal function and offering the potential to examine the course of CKD. PMID: 28355260
  2. this work reveals that TFF2 has tumor-suppressor activity, which may, in part, be regulated by SMAD4. PMID: 27523981
  3. Data show the important functions of TFF2 in the gastric mucus barrier, mucus epithelia, immune system, central nervous system, and during fertilization. It has been shown to interact with the gastric mucin MUC6. [Review] PMID: 26201258
  4. We reproducibly associate higher expression of the ligand-receptor axis of TFF2 and CXCR4 with BRAF V600E-mutant colon cancer PMID: 25899003
  5. protease-activated receptor 4 and Trefoil factor 2 are expressed in human colorectal cancer PMID: 25876034
  6. The structural features of the N-linked N,N'-di-N-acetyllactosediamine-inducing determinant on human TFF2 are discussed. PMID: 25210040
  7. Human TTF2 is a lectin that binds alpha-GlcNAc-capped mucin 6 g with antibiotic activity against Helicobacter pylori. PMID: 25124036
  8. Significantly higher levels of TFF2 were in patients with Multiple Organ Dysfunction Syndrome. PMID: 23628371
  9. There is an association between TFF2 and TFF3 polymorphisms and risk of atrophic gastritis and gastric cancer in Chinese people. PMID: 23933418
  10. Human gastric TFF2 peptide contains an N-linked fucosylated N,N'-diacetyllactosediamine (LacdiNAc) oligosaccharide PMID: 22997242
  11. Report TFF2 expression in normal/diseased pancreas and suggest role in tumor cell migration. PMID: 22286382
  12. Report a novel TFF2 splice variant (EX2TFF2) which correlates with longer overall survival time in cholangiocarcinoma. PMID: 22159958
  13. TFF2 messenger RNA expression is significantly increased in nasal mucosal brushings during asthma exacerbations in children. PMID: 22329990
  14. Data show that frog TFF2 activates protease-activated receptor (PAR) 1 to induce human platelet aggregation, and suggest that human TFF2 promotes cell migration via PAR4. PMID: 21461878
  15. TFF2 is mitogenic in cholangiocarcinoma via EGFR/MAPK activation. PMID: 21472131
  16. TFF2 negatively regulates preneoplastic progression and subsequent tumor development in the stomach, a role that is subverted by promoter methylation during H pylori infection PMID: 20801119
  17. autoinduction of promoter requires an upstream cis-acting element PMID: 12054609
  18. Transcripts not detectable in conjunctiva. Review. PMID: 12613926
  19. expression of TFF2 and Helicobacter pylori infection in carcinogenesis of gastric mucosa PMID: 12717829
  20. The results suggest that TFF2 expression may play a role in gastric cancer invasion and could be a useful target for therapeutic intervention. PMID: 13679442
  21. PPARgamma mediates NSAIDs-induced up-regulation of TFF2 expression in gastric epithelial cells. PMID: 14759512
  22. TFF2 and its putative receptor, DMBT1, were expressed non-specifically in biliary epithelial cells of the damaged small bile ducts, suggesting a cytoprotective role in biliary pathophysiology. PMID: 15101998
  23. TFF2 could play a role in mammary gland tumorigenesis. PMID: 15177880
  24. TFF2 is expressed in normal and malignant breast epithelial cells and it stimulates the migration of breast cancer cells. PMID: 15177883
  25. Epidermal growth factor and trefoil factor family 2 synergistically trigger chemotaxis on BEAS-2B cells via different signaling cascades PMID: 15256384
  26. Demonstration that TFF2 rhythm is impaired in cohorts of individuals known to suffer gastric symptoms suggests that interventions to restore the normal TFF2 rhythm in those with poor mucosal protection could reduce morbidity. PMID: 15984970
  27. TFF2 staining was detected in large, diffuse tumors and in tumors with lymph node metastasis and had a significant correlation with the number of microvessels. PMID: 16166422
  28. human pancreatic polypeptide inhibits TFF2 secretion in a diurnal rhythm PMID: 16359755
  29. Co-localization of TFF2 with gland mucous cell mucin suggests a physical interaction between TFF2 and gland mucous cell mucin. The TFF2 trapped in the adherent mucins may be responsible for mucosal defense, healing, and repair. PMID: 16786324
  30. PPARgamma may be involved in the gastric mucosal defense through regulating TFF2 expression PMID: 17118693
  31. Gastrin regulates TFF2 transcription through a GC-rich DNA-binding site and a protein kinase dependent pathway. PMID: 17332476
  32. CXCR4 as a bona fide signaling receptor for TFF2 and suggest a mechanism through which TFF2 may modulate immune and tumorigenic responses in vivo. PMID: 19064997
  33. The researchers found evidence that H. pylori-associated CAG has a negative effect on the expression of TFF2 in the gastric antrum and may be associated with H. pylori-induced gastric mucosal damage. PMID: 19344006
  34. p53 induces cell apoptosis and inhibits cell migration in part by downregulating TFF2 expression through an AP-1-like site, suggesting that TFF2 may be an important downstream target of p53. PMID: 19541923

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Subcellular Location Secreted
Tissue Specificity Stomach.
Database Links

HGNC: 11756

OMIM: 182590

KEGG: hsa:7032

STRING: 9606.ENSP00000291526

UniGene: Hs.2979


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