Human Trefoil factor 2(TFF2) ELISA kit

Code CSB-EL023432HU
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
trefoil factor 2
Alternative Names
SML 1 ELISA Kit; SML1 ELISA Kit; SP ELISA Kit; Spasmolysin ELISA Kit; Spasmolytic polypeptide ELISA Kit; spasmolytic protein 1 ELISA Kit; TFF 2 ELISA Kit; TFF2 ELISA Kit; TFF2_HUMAN ELISA Kit; trefoil factor 2 (spasmolytic protein 1) ELISA Kit; Trefoil factor 2 ELISA Kit; Trefoil factor 2 precursor ELISA Kit
Abbreviation
TFF2
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell culture supernates
Detection Range
31.25 ng/mL-2000 ng/mL
Sensitivity
7.81 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TFF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 80-92  
1:2 Average % 95  
Range % 90-100  
1:4 Average % 97  
Range % 90-103  
1:8 Average % 92  
Range % 88-97  
Recovery
The recovery of human TFF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-98  
EDTA plasma (n=4) 98 90-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
2000 2.026 2.007 2.017 1.920  
1000 1.364 1.306 1.335 1.238  
500 0.788 0.724 0.756 0.659  
250 0.457 0.427 0.442 0.345  
125 0.262 0.246 0.254 0.157  
62.5 0.175 0.167 0.171 0.074  
31.25 0.119 0.114 0.117 0.020  
0 0.101 0.093 0.097    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human TFF2 ELISA Kit was designed for the quantitative measurement of Human TFF2 protein in serum, plasma, tissue homogenates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 31.25 ng/mL-2000 ng/mL and the sensitivity is 7.81 ng/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Inhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains.
Gene References into Functions
  1. Data show that trefoil factor 2 (TFF2( urine levels continuously decreased with disease progression, TFF2 serum concentrations progressively increased from the early to later chronic kidney disease (CKD) stages, indicating changes in renal function and offering the potential to examine the course of CKD. PMID: 28355260
  2. this work reveals that TFF2 has tumor-suppressor activity, which may, in part, be regulated by SMAD4. PMID: 27523981
  3. Data show the important functions of TFF2 in the gastric mucus barrier, mucus epithelia, immune system, central nervous system, and during fertilization. It has been shown to interact with the gastric mucin MUC6. [Review] PMID: 26201258
  4. We reproducibly associate higher expression of the ligand-receptor axis of TFF2 and CXCR4 with BRAF V600E-mutant colon cancer PMID: 25899003
  5. protease-activated receptor 4 and Trefoil factor 2 are expressed in human colorectal cancer PMID: 25876034
  6. The structural features of the N-linked N,N'-di-N-acetyllactosediamine-inducing determinant on human TFF2 are discussed. PMID: 25210040
  7. Human TTF2 is a lectin that binds alpha-GlcNAc-capped mucin 6 g with antibiotic activity against Helicobacter pylori. PMID: 25124036
  8. Significantly higher levels of TFF2 were in patients with Multiple Organ Dysfunction Syndrome. PMID: 23628371
  9. There is an association between TFF2 and TFF3 polymorphisms and risk of atrophic gastritis and gastric cancer in Chinese people. PMID: 23933418
  10. Human gastric TFF2 peptide contains an N-linked fucosylated N,N'-diacetyllactosediamine (LacdiNAc) oligosaccharide PMID: 22997242
  11. Report TFF2 expression in normal/diseased pancreas and suggest role in tumor cell migration. PMID: 22286382
  12. Report a novel TFF2 splice variant (EX2TFF2) which correlates with longer overall survival time in cholangiocarcinoma. PMID: 22159958
  13. TFF2 messenger RNA expression is significantly increased in nasal mucosal brushings during asthma exacerbations in children. PMID: 22329990
  14. Data show that frog TFF2 activates protease-activated receptor (PAR) 1 to induce human platelet aggregation, and suggest that human TFF2 promotes cell migration via PAR4. PMID: 21461878
  15. TFF2 is mitogenic in cholangiocarcinoma via EGFR/MAPK activation. PMID: 21472131
  16. TFF2 negatively regulates preneoplastic progression and subsequent tumor development in the stomach, a role that is subverted by promoter methylation during H pylori infection PMID: 20801119
  17. autoinduction of promoter requires an upstream cis-acting element PMID: 12054609
  18. Transcripts not detectable in conjunctiva. Review. PMID: 12613926
  19. expression of TFF2 and Helicobacter pylori infection in carcinogenesis of gastric mucosa PMID: 12717829
  20. The results suggest that TFF2 expression may play a role in gastric cancer invasion and could be a useful target for therapeutic intervention. PMID: 13679442
  21. PPARgamma mediates NSAIDs-induced up-regulation of TFF2 expression in gastric epithelial cells. PMID: 14759512
  22. TFF2 and its putative receptor, DMBT1, were expressed non-specifically in biliary epithelial cells of the damaged small bile ducts, suggesting a cytoprotective role in biliary pathophysiology. PMID: 15101998
  23. TFF2 could play a role in mammary gland tumorigenesis. PMID: 15177880
  24. TFF2 is expressed in normal and malignant breast epithelial cells and it stimulates the migration of breast cancer cells. PMID: 15177883
  25. Epidermal growth factor and trefoil factor family 2 synergistically trigger chemotaxis on BEAS-2B cells via different signaling cascades PMID: 15256384
  26. Demonstration that TFF2 rhythm is impaired in cohorts of individuals known to suffer gastric symptoms suggests that interventions to restore the normal TFF2 rhythm in those with poor mucosal protection could reduce morbidity. PMID: 15984970
  27. TFF2 staining was detected in large, diffuse tumors and in tumors with lymph node metastasis and had a significant correlation with the number of microvessels. PMID: 16166422
  28. human pancreatic polypeptide inhibits TFF2 secretion in a diurnal rhythm PMID: 16359755
  29. Co-localization of TFF2 with gland mucous cell mucin suggests a physical interaction between TFF2 and gland mucous cell mucin. The TFF2 trapped in the adherent mucins may be responsible for mucosal defense, healing, and repair. PMID: 16786324
  30. PPARgamma may be involved in the gastric mucosal defense through regulating TFF2 expression PMID: 17118693
  31. Gastrin regulates TFF2 transcription through a GC-rich DNA-binding site and a protein kinase dependent pathway. PMID: 17332476
  32. CXCR4 as a bona fide signaling receptor for TFF2 and suggest a mechanism through which TFF2 may modulate immune and tumorigenic responses in vivo. PMID: 19064997
  33. The researchers found evidence that H. pylori-associated CAG has a negative effect on the expression of TFF2 in the gastric antrum and may be associated with H. pylori-induced gastric mucosal damage. PMID: 19344006
  34. p53 induces cell apoptosis and inhibits cell migration in part by downregulating TFF2 expression through an AP-1-like site, suggesting that TFF2 may be an important downstream target of p53. PMID: 19541923

Show More

Hide All

Subcellular Location
Secreted.
Tissue Specificity
Stomach.
Database Links

HGNC: 11756

OMIM: 182590

KEGG: hsa:7032

STRING: 9606.ENSP00000291526

UniGene: Hs.2979

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1