Recombinant Mouse Fatty acid-binding protein, liver protein(Fabp1) (Active)

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Code CSB-AP000551MO
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Product Details

Purity >95% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The binding affinity of rMuFABP1 for the synthetic ligand cis-parinaric acid has been measured by fluorescence titration. Half maximal fluorescence of 2.5 μM rMuFABP1 is achieved with approximately 5 μM cis-paranaric acid.
Target Names Fabp1
Uniprot No. P12710
Research Area Signal Transduction
Alternative Names Fabp1; FabplFatty acid-binding protein; liver; 14 kDa selenium-binding protein; Fatty acid-binding protein 1; Liver-type fatty acid-binding protein; L-FABP
Species Mus musculus (Mouse)
Source E.coli
Expression Region 1-127aa
Complete Sequence MNFSGKYQLQ SQENFEPFMK AIGLPEDLIQ KGKDIKGVSE IVHEGKKIKL TITYGPKVVR NEFTLGEECE LETMTGEKVK AVVKLEGDNK MVTTFKGIKS VTELNGDTIT NTMTLGDIVY KRVSKRI
Mol. Weight 14.2 kDa
Protein Length Full Length
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, 2 % trehalose
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 5-10 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
(From Uniprot)
Plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes. Binds cholesterol. Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.
Gene References into Functions
  1. https://chalkbeat.org/posts/ny/2018/06/04/a-chalkbeat-cheat-sheet-the-specialized-high-schools-admissions-test-overhaul/ PMID: 28972119
  2. Ablating both Fabp1 and Scp2/Scpx (TKO) induces hepatic phospholipid and cholesterol accumulation in high fat-fed mice PMID: 29307784
  3. role of Fabp1/Scp-2 in hepatic phytol metabolism PMID: 28411199
  4. Individually ablating SCPx or SCP2/SCPx elicited concomitant upregulation of L-FABP. PMID: 27940000
  5. Lack of a significant decrease in the flux of HDL-[(3)H]CE to biliary FC or bile acids in FABP1(-/-) mice indicates the likely compensation of its function by an as yet unidentified mechanism. Taken together, these studies demonstrate that FABP1 and SCP2 facilitate the preferential movement of HDL-CEs to bile for final elimination PMID: 27381048
  6. data showed that Fabp1 gene ablation markedly diminished the impact of high-fat diet on brain endocannabinoid levels, especially in male mice PMID: 27861894
  7. Studies show that despite overall tertiary structure similarity, the hFABP1 differs significantly from rat FABP1 in secondary structure, much larger ligand binding cavity, and affinities/specificities for some ligands. Moreover, while both mouse and hFABP1 mediate ligand induction of PPARA, they differ markedly in genes induced. PMID: 27117865
  8. FABP1 knockout increased brain levels of arachidonic acid-containing endocannabinoids PMID: 27167970
  9. L-FABP was more important in hepatic retention of bile acids, while SCP-2/SCP-x more broadly affected biliary bile acid and phospholipid levels. PMID: 26541319
  10. These findings suggest that some of the phenotypic divergence between the two L-Fabp(-/-) lines may reflect unanticipated differences in genetic background, underscoring the importance of genetic background in phenotypic characterization. PMID: 26251469
  11. Loss of L-FABP and SCP-2, or both induces hepatic lipid accumulation in female mice and mimics non-alcoholic fatty liver disease. PMID: 26116377
  12. attenuates tubulointerstitial damage in aldosterone-induced nephropathy by reducing oxidative stress PMID: 25339700
  13. L-FABP appears to function more in hepatic retention of bile acids as well as hepatic uptake and biliary secretion of HDL-cholesterol PMID: 25277800
  14. L-FABP is not required to channel ATGL-hydrolyzed FAs to mitochondria for beta-oxidation or the nucleus for PPAR-alpha regulation PMID: 24610891
  15. Data show that combined deletion of microsomal triglyceride transfer protein (Mttp) and liver fatty acid binding protein 1 (L-Fabp) are protected from lithogenic diet (LD)-induced gallstone formation. PMID: 24474819
  16. L-Fabp has a role in modifying intestinal fatty acid composition and adenoma formation in ApcMin/+ mice PMID: 23921281
  17. The maximum increase in LFABP expression occurs when stimulation with IL-6 and PPARalpha-ligands takes place simultaneously. PMID: 23534555
  18. liver-type fatty acid-binding protein in the proximal tubules may protect against angiotensin II-induced SSHT by attenuating activation of the intrarenal renin-angiotensin system and reducing oxidative stress and tubulointerstitial inflammation. PMID: 23940201
  19. These data establish that L-FABP is an indirect antioxidant protein essential for sequestering FFA and that its impairment could contribute to in the pathogenesis of alcoholic liver disease . PMID: 23359610
  20. CrPic exhibited amelioration alloxan induced oxidative stress in mouse livers. A significant increase in liver fatty acid-binding protein (L-FABP) was observed, which indicates increased fatty acid utilization in liver tissue PMID: 23603011
  21. Liver-type fatty acid-binding protein gene-ablation exacerbated diet-induced weight gain and fat tissue mass gain in mice fed high-fat diet. PMID: 23539345
  22. L-FABP deletion attenuates both diet-induced hepatic steatosis and fibrogenesis, despite the observation that L-Fabp paradoxically promotes fatty acid and lipid droplet accumulation and inhibits hepatic stellate cell activation in vitro. PMID: 23401290
  23. L-FABP's importance in fibrate-induction of hepatic PPARalpha LCFA beta-oxidative genes, especially in the context of high glucose levels. PMID: 23747828
  24. Aged female L-Fabp-/- mice are protected against weight gain and hepatic steatosis. PMID: 22327204
  25. liver fatty acid-binding protein, the single most prevalent hepatic cytosolic protein that binds cholesterol, was upregulated twofold in SCP-2 null hepatocytes PMID: 22241858
  26. L-FABP gene ablation decreased fat oxidation and sensitized all mice to weight gain as whole body fat tissue mass (FTM) and LTM-with the most gain observed in FTM of control vs high-fat fed female L-FABP null mice. PMID: 20035485
  27. enhanced uptake and intracellular targeting of long and medium chain fatty acids to the nucleus PMID: 12023965
  28. L-FABP is an important determinant of hepatic lipid composition and turnover PMID: 12670956
  29. data point to an inducible defect in fatty acid utilization in fasted L-Fabp-/- mice that involves targeting of substrate for use in triglyceride metabolism PMID: 14534295
  30. under fasting conditions, hepatic L-FABP contributes to hepatic long chain fatty acid oxidation and ketogenesis by a nontranscriptional mechanism, whereas L-FABP can activate ketogenic gene expression in fed mice PMID: 14656998
  31. L-FABP may function as a carrier for selectively enhancing the distribution of long-chain fatty acyl CoA (LCFA-CoA), as well as LCFA, to nuclei for potential interaction with nuclear receptors. PMID: 14992586
  32. results with cultured primary hepatocytes isolated from L-FABP (+/+) and L-FABP (-/-) mice demonstrated a physiological role of L-FABP in the uptake and metabolism of branched-chain fatty acids PMID: 15155724
  33. gene expression of liver-type FABP was independent of PPARalpha and may have general implications for the activation of PPARgamma in alveolar macrophages PMID: 15203117
  34. Data show that liver fatty acid protein gene ablation exerts a significant role, especially in female mice, in branched-chain fatty acid metabolism. PMID: 15692150
  35. results suggesting a physiological role for the major cytosolic bile acid-binding protein (L-FABP) in influencing liver bile metabolic phenotype and gall-bladder bile lipids of male mice, especially in response to dietary cholesterol PMID: 15984932
  36. L-FABP is involved in the physiological regulation of cholesterol metabolism, body weight gain, and obesity. PMID: 16123197
  37. reducing both L-FABP and microsomal triglyceride transfer protein is an effective means to reduce very low density lipoprotein secretion without causing hepatic steatosis PMID: 16950764
  38. Inactivation of Gata4 or Hnf1alpha had a partial effect (approximately 50% reduction) on Fabp1 gene expression during cytodifferentiation and suckling. PMID: 17272516
  39. L-FABP can select cargo for and bud PCTV from intestinal ER membranes PMID: 17449472
  40. inhibition of CYP2E1 and regulation of L-FABP by Platycodi radix play an important role in alcohol-induced hepatoprotection PMID: 17587688
  41. L-Fabp(-/-) mice are protected against Western diet-induced obesity and hepatic steatosis. PMID: 18032478
  42. Knockout female C57BL mice exhibit increased obesity in aging. PMID: 18806093
  43. expression of renal hL-FABP was upregulated in the diabetic Tg mice and protective against tubulointerstitial damage of diabetic nephropathy PMID: 18854419
  44. role for L-FABP as an important physiological regulator of PPARalpha PMID: 19104910
  45. Together these data indicate a role for L-FABP in intestinal trafficking of both saturated (SFA),and cholesterol. PMID: 19116776
  46. Data suggest that changes in hepatic cholesterol metabolism and biliary lipid secretion as well as changes in enterohepatic BA metabolism increase gallstone susceptibility in LD fed L-Fabp(-/-) mice. PMID: 19136665
  47. studies support the hypothesis that L-FABP may facilitate ligand (LCFA)-activated PPARalpha transcriptional activity at least in part by increasing total LCFA ligand available to PPARalpha PMID: 19285478
  48. studies consistent with L-FABP regulating PPARalpha transcriptional activity in hepatocytes through direct interaction with PPARalpha; findings suggest role for L-FABP interaction with PPARalpha in long chain fatty acid metabolism PMID: 19289416
  49. renal expression and urinary excretion of hL-FABP significantly reflected the severity of tubulointerstitial damage in FA-induced nephropathy. PMID: 19435794
  50. Data support the hypothesis that L-FABP plays a role in physiological regulation of not only hepatic fatty acid metabolism, but also that of hepatic cholesterol. PMID: 19815623

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Subcellular Location Cytoplasm
Protein Families Calycin superfamily, Fatty-acid binding protein (FABP) family
Database Links

KEGG: mmu:14080

STRING: 10090.ENSMUSP00000064655

UniGene: Mm.22126

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