NELFB Antibody

Datasheet
Code CSB-PA03765A0Rb
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Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. Q8WX92
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Image
  • Western blot
    All lanes: Negative elongation factor B antibody at 2ug/ml
    Lane 1: EC109whole cell lysate
    Lane 2: 293T whole cell lysate
    Secondary
    Goat polyclonal to Rabbit IgG at 1/15000 dilution
    Predicted band size: 64 kDa
    Observed band size: 64 kDa

  • Western Blot
    All lanes: Negative elongation factor B antibody at 2ug/ml
    Lane 1: NIH/3T3 whole cell lysate
    Lane 2: HL60 whole cell lysate
    Secondary
    Goat polyclonal to Rabbit IgG at 1/15000 dilution
    Predicted band size: 64 kDa
    Observed band size: 64 kDa

  • Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA03765A0Rb at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA03765A0Rb at dilution of 1:100

  • Chromatin Immunoprecipitation Hela(1.1*106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-COBRA1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers (CSB-PP03765HU) against the TFF1 promoter.

Immunogen Recombinant human Negative elongation factor B protein (8-199AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, ChIP; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200
Relevance Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin unfolding.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Negative elongation factor B (NELF-B) (Cofactor of BRCA1), NELFB, COBRA1 KIAA1182
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Research Area Epigenetics and Nuclear Signaling
Target Names NELFB
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Function Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Binds RNA which may help to stabilize the NELF complex on nucleic acid. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. May be able to induce chromatin unfolding.
Subcellular location Nucleus

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