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Recombinant Acidianus two-tailed virus Structural protein ORF145

Recombinant Acidianus two-tailed virus Structural protein ORF145

Code	CSB-YP667613ACAM
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP667613ACAM
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP667613ACAM-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP667613ACAM
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP667613ACAM
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Uniprot No.

Q3V4R7

Alternative Names

RNAP inhibitory protein; RIP

Species

Acidianus two-tailed virus (ATV)

Expression Region

1-145

Target Protein Sequence

MKNMLHPQKY ETHVLDDLME FYEGVIGYPE IDLRLAGEEA WLKGVNPELA EAVKKIIKTI RRYLEGSPYD GSEKPIPRYI IAEIFSQIAP EVQLLVNALD TEGKYGFLKH IKKLNLNSLA MLSKNYNEND KLWKELENEG YVYLE

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Plays a role in the inhibition of global transcription by interacting with the RNA polymerase clamp, locking it in a fixed position and inhibiting the formation and/or stability of the preinitiation complex (PIC).

Gene References into Functions

ORF145 from the temperate Acidianus two-tailed virus (ATV) forms a high-affinity complex with RNAP by binding inside the DNA-binding channel where it locks the flexible RNAP clamp in one position. This counteracts the formation of transcription pre-initiation complexes in vitro and represses abortive and productive transcription initiation, as well as elongation. Both host and viral promoters are subjected to ORF145 repre PMID: 27882920

Subcellular Location

Virion.

Database Links

KEGG: vg:4484253

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