Recombinant Ajellomyces capsulata Nuclear distribution protein PAC1 (PAC1)

Code CSB-YP505698AYZ
MSDS
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Source Yeast
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Code CSB-EP505698AYZ
MSDS
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Source E.coli
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Code CSB-EP505698AYZ-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP505698AYZ
MSDS
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Source Baculovirus
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Code CSB-MP505698AYZ
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PAC1
Uniprot No.
Alternative Names
PAC1; LIS1; HCBG_05556; Nuclear distribution protein PAC1; Lissencephaly-1 homolog; LIS-1; nudF homolog
Species
Ajellomyces capsulatus (strain G186AR / H82 / ATCC MYA-2454 / RMSCC 2432) (Darling's disease fungus) (Histoplasma capsulatum)
Expression Region
1-456
Target Protein Sequence
MARLLTNRQA DELHKSIIAY LSANDLPNTA AALRAELNLT EEDFDATAVK KYETLLEKKW TSIVRLQKKI MDLEARNAAL QSELDNLTPA SLLKPRRDPT SWLPTHPPRY SLESHRDTIN CIAFHPKFSS LASGSDDFTI KIWDWELGEL EMTLKGHTRA VLDVDYGSPQ GITMLASCSS DLSVKLWDPA EGYKNVRTLL GHDHSVSAVR FIPCRNLLAS ASRDKDVRIW DVTTGYCVRS IQGHTGWVRD VCPSFDGNFL FSSGDDMTAR LWDISAIPNP ENKVTMAGHE HFIECCALAP PTSYQYLAPV AGLKGPSYPK SAAEFMATGS RDKTIKVWDV RGTCLMTLIG HDNWVRGIVF HPAGKYLLSV SDDRTLRCWD LSQEGKCVKT IKDAHDRFVT CLSWAPGVAK DVPAIPSNTA KGESSEIKET LKSPDVQIRC VIATGSVDRK LQIFAA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for nuclear migration during vegetative growth as well as development. Required for retrograde early endosome (EE) transport from the hyphal tip. Required for localization of dynein to the mitotic spindle poles. Recruits additional proteins to the dynein complex at SPBs.
Subcellular Location
Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle pole.
Protein Families
WD repeat LIS1/nudF family
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