Recombinant Arabidopsis thaliana Ankyrin repeat domain-containing protein 2 (AKR2)

Code CSB-YP874372DOA
MSDS
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Source Yeast
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Code CSB-EP874372DOA
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Source E.coli
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Code CSB-EP874372DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP874372DOA
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Source Baculovirus
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Code CSB-MP874372DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AKR2A
Uniprot No.
Alternative Names
AKR2A; AFT; AKR2; At4g35450; F15J1.20Ankyrin repeat domain-containing protein 2A; AtAKR2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-342
Target Protein Sequence
MASNSEKNPL LSDEKPKSTE ENKSSKPESA SGSSTSSAMP GLNFNAFDFS NMASILNDPS IREMAEQIAK DPAFNQLAEQ LQRSIPNAGQ EGGFPNFDPQ QYVNTMQQVM HNPEFKTMAE KLGTALVQDP QMSPFLDAFS NPETAEHFTE RMARMKEDPE LKPILDEIDA GGPSAMMKYW NDPEVLKKLG EAMGMPVAGL PDQTVSAEPE VAEEGEEEES IVHQTASLGD VEGLKAALAS GGNKDEEDSE GRTALHFACG YGELKCAQVL IDAGASVNAV DKNKNTPLHY AAGYGRKECV SLLLENGAAV TLQNLDEKTP IDVAKLNSQL EVVKLLEKDA FL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Exhibits chaperone activity toward chloroplast outer envelope membrane, mitochondrion outer membrane, endoplasmic reticulum membrane and peroxisomal proteins, by recruiting specific proteins containing a single transmembrane associated with an AKR2A-binding sequence (ABS) and subsequently binding glycolipids (e.g. monogalactosyldiacylglycerol (MGDG) and phosphatidylglycerol (PG)) present in the membrane of the target organelle. Seems to be involved in the regulation of hydrogen peroxide levels during biotic and abiotic stresses by optimizing the ascorbate peroxidase 3 (APX3) hydrogen peroxide-degrading activity. This regulation might be monitored by GRF6. Cytosolic targeting factor for chloroplast outer membrane (COM) proteins that mediates sorting and targeting of nascent chloroplast outer envelope membrane (OEM) proteins to the chloroplast. Facilitates the targeting of OEP7 to chloroplasts. Facilitates the targeting of APX3 to peroxisomes. Involved in cellular metabolism (e.g. peroxisome activity) and required for plant growth and development.
Gene References into Functions
  1. AKR2A-mediated targeting of chloroplast outer membrane proteins is coupled to their translation, which in turn is crucial for biogenesis of the entire chloroplast proteome. PMID: 25880450
  2. the AKR2 ankyrin repeat domain drives chloroplast targeting through coincident binding of two chloroplast lipids PMID: 25203210
  3. The role of AKR2A in the regulation of at least 500 genes in the Arabidopsis genome is discussed. PMID: 21057222
  4. AKR2A is a molecular chaperone that binds specifically to APX3 and to five other single-membrane spanning proteins. PMID: 20215589
  5. AKR2A functions as a cytosolic mediator for sorting and targeting of nascent chloroplast OEM proteins to the chloroplast. PMID: 18193034

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Subcellular Location
Cytoplasm. Nucleus. Plastid, chloroplast outer membrane; Peripheral membrane protein; Cytoplasmic side.
Tissue Specificity
Ubiquitously expressed at basal level.
Database Links

KEGG: ath:AT4G35450

STRING: 3702.AT4G35450.5

UniGene: At.24072

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