Recombinant Arabidopsis thaliana Transcription factor TCP15 (TCP15)

Code CSB-YP863241DOA
MSDS
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Source Yeast
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Code CSB-EP863241DOA
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Source E.coli
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Code CSB-EP863241DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863241DOA
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Source Baculovirus
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Code CSB-MP863241DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TCP15
Uniprot No.
Alternative Names
TCP15; At1g69690; T6C23.11; Transcription factor TCP15; AtTCP15
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-325
Target Protein Sequence
MDPDPDHNHR PNFPLQLLDS STSSSSTSLA IISTTSEPNS EPKKPPPKRT STKDRHTKVE GRGRRIRMPA MCAARVFQLT RELGHKSDGE TIEWLLQQAE PAVIAATGTG TIPANFTSLN ISLRSSRSSL SAAHLRTTPS SYYFHSPHQS MTHHLQHQHQ VRPKNESHSS SSSSSQLLDH NQMGNYLVQS TAGSLPTSQS PATAPFWSSG DNTQNLWAFN INPHHSGVVA GDVYNPNSGG SGGGSGVHLM NFAAPIALFS GQPLASGYGG GGGGGGEHSH YGVLAALNAA YRPVAETGNH NNNQQNRDGD HHHNHQEDGS TSHHS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor involved the regulation of plant development. Together with TCP14, modulates plant stature by promoting cell division in young internodes. Represses cell proliferation in developing leaf blade and specific floral tissues. Together with TCP15, acts downstream of gibberellin (GA), and the stratification pathways that promote seed germination. Involved in the control of cell proliferation at the root apical meristem (RAM) by regulating the activity of CYCB1-1. Acts together with SPY to promote cytokinin responses that affect leaf shape and trichome development in flowers. Involved in gynoecium and silique development. Modulates the development of the different tissues of the gynoecium through its participation in auxin and cytokinin responses. Modulates the expression of the cytokinin-responsive genes ARR7 and ARR15. May repress the expression of the auxin biosynthetic genes YUC1 and YUC4. Acts as negative regulator of anthocyanin accumulation under high light conditions. Modulates the expression of transcription factors involved in the induction of anthocyanin biosynthesis genes. Transcription factor involved in the regulation of endoreduplication. Represses endoreduplication by activating the gene expression of the key cell-cycle regulators RBR1 and CYCA2-3. Regulates the expression of the defense gene pathogenesis-related protein 2 (PR2) in antagonism to SRFR1, a negative regulator of effector-triggered immunity.
Gene References into Functions
  1. TCP15 acts as a repressor of anthocyanin accumulation acting on the expression of anthocyanin biosynthesis genes and upstream transcriptional regulators. PMID: 26574599
  2. TCP15-dependent transcription is sensitive to oxidative stress. PMID: 23686421
  3. TCP15 regulates the expression of boundary-specific genes through a pathway that affects auxin homeostasis and partially overlaps with the one modulated by class II CIN-like TCP proteins. PMID: 22016421
  4. TCP15 regulates endoreduplication by modulating expression of key cell-cycle genes. PMID: 21992944
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in archespores, pollen mother cell, ovule primordia and megaspore mother cells. Expressed in young proliferating tissues. Expressed in developing embryos, and seeds during germination.
Database Links

KEGG: ath:AT1G69690

STRING: 3702.AT1G69690.1

UniGene: At.23272

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