Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) TCP15 Polyclonal antibody

Uniprot No.

Q9C9L2

Target Names

TCP15

Alternative Names

TCP15 antibody; At1g69690 antibody; T6C23.11 antibody; Transcription factor TCP15 antibody; AtTCP15 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana TCP15 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Transcription factor involved the regulation of plant development. Together with TCP14, modulates plant stature by promoting cell division in young internodes. Represses cell proliferation in developing leaf blade and specific floral tissues. Together with TCP15, acts downstream of gibberellin (GA), and the stratification pathways that promote seed germination. Involved in the control of cell proliferation at the root apical meristem (RAM) by regulating the activity of CYCB1-1. Acts together with SPY to promote cytokinin responses that affect leaf shape and trichome development in flowers. Involved in gynoecium and silique development. Modulates the development of the different tissues of the gynoecium through its participation in auxin and cytokinin responses. Modulates the expression of the cytokinin-responsive genes ARR7 and ARR15. May repress the expression of the auxin biosynthetic genes YUC1 and YUC4. Acts as negative regulator of anthocyanin accumulation under high light conditions. Modulates the expression of transcription factors involved in the induction of anthocyanin biosynthesis genes. Transcription factor involved in the regulation of endoreduplication. Represses endoreduplication by activating the gene expression of the key cell-cycle regulators RBR1 and CYCA2-3. Regulates the expression of the defense gene pathogenesis-related protein 2 (PR2) in antagonism to SRFR1, a negative regulator of effector-triggered immunity.

Gene References into Functions

TCP15 acts as a repressor of anthocyanin accumulation acting on the expression of anthocyanin biosynthesis genes and upstream transcriptional regulators. PMID: 26574599
TCP15-dependent transcription is sensitive to oxidative stress. PMID: 23686421
TCP15 regulates the expression of boundary-specific genes through a pathway that affects auxin homeostasis and partially overlaps with the one modulated by class II CIN-like TCP proteins. PMID: 22016421
TCP15 regulates endoreduplication by modulating expression of key cell-cycle genes. PMID: 21992944

Subcellular Location

Nucleus.

Tissue Specificity

Expressed in archespores, pollen mother cell, ovule primordia and megaspore mother cells. Expressed in young proliferating tissues. Expressed in developing embryos, and seeds during germination.

Database Links

KEGG: ath:AT1G69690

STRING: 3702.AT1G69690.1

UniGene: At.23272

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TCP15 Antibody

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