Recombinant Arabidopsis thaliana Dynamin-related protein 1A (DRP1A), partial

Code CSB-YP337176DOA
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Source Yeast
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Code CSB-EP337176DOA
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Source E.coli
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Code CSB-EP337176DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP337176DOA
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Source Baculovirus
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Code CSB-MP337176DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DRP1A
Uniprot No.
Alternative Names
DRP1A; ADL1; ADL1A; AG68; RSW9; At5g42080; MJC20.19Dynamin-related protein 1A; Dynamin-like protein 1; Dynamin-like protein A; Protein RADIAL SWELLING 9
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Microtubule-associated force-producing protein that is targeted to at the leading edges of the forming cell plate during cytokinesis. Plays also a major role in plasma membrane maintenance and cell wall integrity with implications in vesicular trafficking, polar cell expansion, vascular formation, and other aspects of plant growth and development, including stigmatic papillae expansion. Collaboratively with DRP2B, participates in clathrin-coated vesicle formation during endocytosis. Necessary for BOR1 polar localization in low-boron (B) conditions as well as for BOR1 endocytosis and subsequent degradation under high-concentration of boron. Has a GTPase activity. Required for the sterols-dependent dynamic high lipid order observed at the cell plate of dividing cells. Together with SH3P2, converts the fused vesicles to tubular structures at the cell plate and phragmoplasts during cytokinesis. With DRP2B and PIP5K3, required for the precise coordination of polar ARAC3/ROP6 and ARAC4/ROP2 placement and subsequent root hair positioning during planar polarity formation in root hair-forming cells, probably by mediating the correct basal-to-planar polarity switching of D6PK into the polar, lipid-enriched domain. Involved in endocytosis required for cellulose deposition during cell wall formation and elongation. Interacts with plasma membrane-mimetic liposomes and induces their clustering.
Gene References into Functions
  1. DRP1A is required for boron-induced degradation of BOR1. PMID: 27449211
  2. DRP1A is required for high membrane lipid order at the cell plate. PMID: 25234576
  3. propose that rapid and specific internalization of PIN proteins mediated by the DRP1 proteins and the associated CME machinery from the cell plate membranes during cytokinesis is an important mechanism for proper polar PIN positioning in interphase cells PMID: 21658946
  4. DRP2B shares domain organization with animal dynamins whereas DRP1A is plant-specific. PMID: 20231465
  5. purified AtDRP1A behaves very differently than purified dynamin, and in ways that are difficult to reconcile with what is known of its in vivo activity, suggesting that additional factors or modifications are needed for AtDRP1A to function. PMID: 20171176
  6. DRP1 modulates the VAN3 function in vesicle budding from the trans-Golgi network, which regulates vascular formation in Arabidopsis. [DRP1A] PMID: 15923323
  7. absence causes impaired endocytosis which may contribute to the development of shorter, fatter roots, probably by reducing cellulose synthesis PMID: 18256049
  8. DRP1A forms discreet dynamic foci in the epidermal cell cortex and while the foci overlap with those formed by DRP1C and clathrin light chain, there are clear differences in behavior and response to pharmacological inhibitors between DRP1A and DRP1C foci. PMID: 18344418
  9. These results indicate that Arabidopsis dynamin-related proteins, DRP1A and DRP2B, from two different families, participate in membrane remodeling at a similar place in the cell plate. PMID: 18612642

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Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, phragmoplast. Cytoplasm, cell cortex. Cytoplasmic vesicle, clathrin-coated vesicle. Cell membrane.
Protein Families
TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family
Tissue Specificity
Ubiquitous. Expressed in leaves (at protein level).
Database Links

KEGG: ath:AT5G42080

STRING: 3702.AT5G42080.1

UniGene: At.10365

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