Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DRP1A Polyclonal antibody

Uniprot No.

P42697

Target Names

DRP1A

Alternative Names

DRP1A antibody; ADL1 antibody; ADL1A antibody; AG68 antibody; RSW9 antibody; At5g42080 antibody; MJC20.19Dynamin-related protein 1A antibody; Dynamin-like protein 1 antibody; Dynamin-like protein A antibody; Protein RADIAL SWELLING 9 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana DRP1A protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Microtubule-associated force-producing protein that is targeted to at the leading edges of the forming cell plate during cytokinesis. Plays also a major role in plasma membrane maintenance and cell wall integrity with implications in vesicular trafficking, polar cell expansion, vascular formation, and other aspects of plant growth and development, including stigmatic papillae expansion. Collaboratively with DRP2B, participates in clathrin-coated vesicle formation during endocytosis. Necessary for BOR1 polar localization in low-boron (B) conditions as well as for BOR1 endocytosis and subsequent degradation under high-concentration of boron. Has a GTPase activity. Required for the sterols-dependent dynamic high lipid order observed at the cell plate of dividing cells. Together with SH3P2, converts the fused vesicles to tubular structures at the cell plate and phragmoplasts during cytokinesis. With DRP2B and PIP5K3, required for the precise coordination of polar ARAC3/ROP6 and ARAC4/ROP2 placement and subsequent root hair positioning during planar polarity formation in root hair-forming cells, probably by mediating the correct basal-to-planar polarity switching of D6PK into the polar, lipid-enriched domain. Involved in endocytosis required for cellulose deposition during cell wall formation and elongation. Interacts with plasma membrane-mimetic liposomes and induces their clustering.

Gene References into Functions

DRP1A is required for boron-induced degradation of BOR1. PMID: 27449211
DRP1A is required for high membrane lipid order at the cell plate. PMID: 25234576
propose that rapid and specific internalization of PIN proteins mediated by the DRP1 proteins and the associated CME machinery from the cell plate membranes during cytokinesis is an important mechanism for proper polar PIN positioning in interphase cells PMID: 21658946
DRP2B shares domain organization with animal dynamins whereas DRP1A is plant-specific. PMID: 20231465
purified AtDRP1A behaves very differently than purified dynamin, and in ways that are difficult to reconcile with what is known of its in vivo activity, suggesting that additional factors or modifications are needed for AtDRP1A to function. PMID: 20171176
DRP1 modulates the VAN3 function in vesicle budding from the trans-Golgi network, which regulates vascular formation in Arabidopsis. [DRP1A] PMID: 15923323
absence causes impaired endocytosis which may contribute to the development of shorter, fatter roots, probably by reducing cellulose synthesis PMID: 18256049
DRP1A forms discreet dynamic foci in the epidermal cell cortex and while the foci overlap with those formed by DRP1C and clathrin light chain, there are clear differences in behavior and response to pharmacological inhibitors between DRP1A and DRP1C foci. PMID: 18344418
These results indicate that Arabidopsis dynamin-related proteins, DRP1A and DRP2B, from two different families, participate in membrane remodeling at a similar place in the cell plate. PMID: 18612642

Hide All

Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, phragmoplast. Cytoplasm, cell cortex. Cytoplasmic vesicle, clathrin-coated vesicle. Cell membrane.

Protein Families

TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family

Tissue Specificity

Ubiquitous. Expressed in leaves (at protein level).

Database Links

KEGG: ath:AT5G42080

STRING: 3702.AT5G42080.1

UniGene: At.10365

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DRP1A Antibody

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