Recombinant Arabidopsis thaliana Heat shock 70 kDa protein 1 (HSP70-1), partial

Code CSB-YP334938DOA
MSDS
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Source Yeast
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Code CSB-EP334938DOA
MSDS
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Source E.coli
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Code CSB-EP334938DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP334938DOA
MSDS
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Source Baculovirus
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Code CSB-MP334938DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MED37E
Uniprot No.
Alternative Names
HSP70-1; ERD2; HSC70-1; MED37_4; MED37E; At5g02500; T22P11.90Heat shock 70 kDa protein 1; Heat shock cognate 70 kDa protein 1; Heat shock cognate protein 70-1; AtHsc70-1; Heat shock protein 70-1; AtHsp70-1; Protein EARLY-RESPONSIVE TO DEHYDRATION 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
In cooperation with other chaperones, Hsp70s are key components that facilitate folding of de novo synthesized proteins, assist translocation of precursor proteins into organelles, and are responsible for degradation of damaged protein under stress conditions (Probable). Probably involved in defense response. Chaperone involved in protein targeting to chloroplasts. May cooperate with SGT1 and HSP90 in R gene-mediated resistance towards the oomycete Hyaloperonospora parasitica (downy mildew). Plays a role with WPP-domain proteins in facilitating WIT1 nuclear envelope targeting. Modulates stomatal aperture in response to various environmental conditions and physiological responses to the hormone abscisic acid (ABA).
Gene References into Functions
  1. Here, the authors determined crystal structures of AtBAG5 alone and in complex with Ca(2+)-free CaM. Structural and biochemical studies revealed that Ca(2+)-free CaM and Hsc70 bind AtBAG5 independently, whereas Ca(2+)-saturated CaM and Hsc70 bind AtBAG5 with negative cooperativity. PMID: 27539741
  2. Data conclude that an HSC70-SGT1 chaperone complex is important for multiple plant environmental responses and that the evolutionarily conserved SGS domain of SGT1 is a key determinant of the HSC70-SGT1 association. PMID: 18065690
  3. HSC70-1 is involved in development and abiotic stress responses in Arabidopsis. PMID: 19443614
Subcellular Location
Cytoplasm, cytosol. Nucleus.
Protein Families
Heat shock protein 70 (TC 1.A.33) family, DnaK subfamily
Tissue Specificity
Expressed at a substantial level during normal growth in root, stem, leaf and flower tissues, but not in siliques. Expressed in cotyledons, leaves, stems, vascular bundles, roots, stigmas and anthers.
Database Links

KEGG: ath:AT5G02500

STRING: 3702.AT5G02500.1

UniGene: At.23663

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