Recombinant Arabidopsis thaliana Magnesium-chelatase subunit chlI, chloroplastic(CHLI)

Code CSB-YP322829DOA
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Source Yeast
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Code CSB-EP322829DOA
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Source E.coli
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Code CSB-EP322829DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP322829DOA
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Source Baculovirus
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Code CSB-MP322829DOA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names CHLI1
Uniprot No. P16127
Alternative Names CHLI1; CH42; CS; At4g18480; F28J12.140Magnesium-chelatase subunit ChlI-1; chloroplastic; Mg-chelatase subunit I-1; EC 6.6.1.1; Mg-protoporphyrin IX chelatase subunit ChlI-1; Protein CHLORINA 42
Species Arabidopsis thaliana (Mouse-ear cress)
Expression Region 61-424
Target Protein Sequence VMNVATEINS TEQVVGKFDS KKSARPVYPF AAIVGQDEMK LCLLLNVIDP KIGGVMIMGD RGTGKSTTVR SLVDLLPEIN VVAGDPYNSD PIDPEFMGVE VRERVEKGEQ VPVIATKINM VDLPLGATED RVCGTIDIEK ALTEGVKAFE PGLLAKANRG ILYVDEVNLL DDHLVDVLLD SAASGWNTVE REGISISHPA RFILIGSGNP EEGELRPQLL DRFGMHAQVG TVRDADLRVK IVEERARFDS NPKDFRDTYK TEQDKLQDQI STARANLSSV QIDRELKVKI SRVCSELNVD GLRGDIVTNR AAKALAALKG KDRVTPDDVA TVIPNCLRHR LRKDPLESID SGVLVSEKFA EIFS
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Involved in chlorophyll biosynthesis. Catalyzes the insertion of magnesium ion into protoporphyrin IX to yield Mg-protoporphyrin IX. The magnesium-chelatase is a complex of three subunits, CHLI, CHLD and CHLH. The reaction takes place in two steps, with an ATP-dependent activation followed by an ATP-dependent chelation step. Possesses high affinity for ATP and may play a major role in chlorophyll biosynthesis. Does not bind abscisic acid (ABA), but is a positive regulator of ABA signaling.
Gene References into Functions
  1. The chlorophyll synthesis enzymes, Mg-chelatase complex and Mg-protoporphyrin IX methyltransferase, specifically affect abscisic acid (ABA) signaling in the control of stomatal aperture and have no effect on ABA-induced gene expression. PMID: 24840863
  2. Gun4 is involved in the stimulation of Mg-chelatase activity. PMID: 15884974
  3. Comparison of the amino acid sequence of CHLI 1 and CHLI 2 encoded in the genome of acifluorfen herbicide resistant mutant aci5 and wild type revealed in particular alterations of the C-terminal end. PMID: 17123104
  4. CHLI2 also has ATPase activity but with a lower Vmax and higher Km ATP than CHLI1 PMID: 18846282
  5. CHLI2 could support some chlorophyll biosynthesis in the complete absence of CHLI1. PMID: 19363094
  6. GUN4 stimulating chlorophyll biosynthesis not only by activating Mg-chelatase but also by promoting interactions between ChlH and chloroplast membranes PMID: 19605356
  7. Paths of evolutionary dynamics for CHLI 1 and CHLI 2 genes determining different forms of subunit I of Mg-chelatase complex of Arabidopsis thaliana are proposed. PMID: 19899639

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Subcellular Location Plastid, chloroplast
Protein Families Mg-chelatase subunits D/I family
Database Links

KEGG: ath:AT4G18480

STRING: 3702.AT4G18480.1

UniGene: At.27665

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