CHLI1 Antibody

Datasheet

Code	CSB-PA322829XA01DOA
Size	US$299
Image	Western Blot Positive WB detected in Recombinant protein All lanes: CHLI1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35.1 kDa Observed band size: 35 kDa
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Target Background

Product Details

Uniprot No.

P16127

Target Names

CHLI1

Alternative Names

Magnesium-chelatase subunit ChlI-1, chloroplastic (Mg-chelatase subunit I-1) (EC 6.6.1.1) (Mg-protoporphyrin IX chelatase subunit ChlI-1) (Protein CHLORINA 42) CHLI1 CH42 CS At4g18480 F28J12.140

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CHLI1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Protein G

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The CHLI1 polyclonal antibody is obtained by immunizing a rabbit with a recombinant Saccharomyces cerevisiae CHLI1 protein, which triggers an antibody response. The rabbit serum is collected and then subjected to protein G affinity chromatography purification to harvest the CHLI1 polyclonal antibodies. The effectiveness of the CHLI1 antibody in detecting Saccharomyces cerevisiae CHLI1 protein is evaluated through ELISA and WB assays.

The CHLI1 protein in Arabidopsis thaliana is a subunit of magnesium chelatase, an enzyme complex involved in the biosynthesis of chlorophyll. Chlorophyll is a green pigment essential for photosynthesis, the process by which plants convert light energy into chemical energy.

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Target Background

Function

Involved in chlorophyll biosynthesis. Catalyzes the insertion of magnesium ion into protoporphyrin IX to yield Mg-protoporphyrin IX. The magnesium-chelatase is a complex of three subunits, CHLI, CHLD and CHLH. The reaction takes place in two steps, with an ATP-dependent activation followed by an ATP-dependent chelation step. Possesses high affinity for ATP and may play a major role in chlorophyll biosynthesis. Does not bind abscisic acid (ABA), but is a positive regulator of ABA signaling.

Gene References into Functions

The chlorophyll synthesis enzymes, Mg-chelatase complex and Mg-protoporphyrin IX methyltransferase, specifically affect abscisic acid (ABA) signaling in the control of stomatal aperture and have no effect on ABA-induced gene expression. PMID: 24840863
Gun4 is involved in the stimulation of Mg-chelatase activity. PMID: 15884974
Comparison of the amino acid sequence of CHLI 1 and CHLI 2 encoded in the genome of acifluorfen herbicide resistant mutant aci5 and wild type revealed in particular alterations of the C-terminal end. PMID: 17123104
CHLI2 also has ATPase activity but with a lower Vmax and higher Km ATP than CHLI1 PMID: 18846282
CHLI2 could support some chlorophyll biosynthesis in the complete absence of CHLI1. PMID: 19363094
GUN4 stimulating chlorophyll biosynthesis not only by activating Mg-chelatase but also by promoting interactions between ChlH and chloroplast membranes PMID: 19605356
Paths of evolutionary dynamics for CHLI 1 and CHLI 2 genes determining different forms of subunit I of Mg-chelatase complex of Arabidopsis thaliana are proposed. PMID: 19899639

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Subcellular Location

Plastid, chloroplast.

Protein Families

Mg-chelatase subunits D/I family

Database Links

KEGG: ath:AT4G18480

STRING: 3702.AT4G18480.1

UniGene: At.27665

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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CHLI1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background