Recombinant Bacillus subtilis NTD biosynthesis operon protein NtdA (ntdA)

Code CSB-YP519197BRJ
MSDS
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Source Yeast
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Code CSB-EP519197BRJ
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Source E.coli
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Code CSB-EP519197BRJ-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP519197BRJ
MSDS
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Source Baculovirus
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Code CSB-MP519197BRJ
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ntdA
Uniprot No.
Alternative Names
ntdA; yhjL; BSU10550; 3-oxo-glucose-6-phosphate:glutamate aminotransferase; EC 2.6.1.104; 3-dehydro-glucose-6-phosphate--glutamate transaminase; Kanosamine 6-phosphate transaminase
Species
Bacillus subtilis (strain 168)
Expression Region
1-441
Target Protein Sequence
MQKQVKISGK SKENMSLLKH LKGDVQGKEL VIEDSIVNER WKQVLKEKID IEHDLFNYQK NREISKVPFL PVDRLITNDE VEDILNTLTE VLPTGKFTSG PYLEQFEKVL STYLHKRYVI ATSSGTDAIM IGLLALGLNP GDEVIMPANS FSATENAVLA SGGVPIYVDI NPQTFCIDPD KIEEAITPYT KFILPVHLYG KHSDMQHIRQ IANRYKLKVI EDACQGIGLT DLGKYADITT LSFNPYKNFG VCGKAGAIAT DNEELAKKCI QFSYHGFEVN VKNKKVINFG FNSKMDNLQA AIGLERMKYL SLNNFKRLFL ADRYITQLAE LQNKGYIELP ELSEDHVWHL FPIKVRTEDR ADIMTKLNED FGVQTDVYYP ILSHMQKTPL VQDKYAGLQL VHTEKAHSQV LHLPLYPSFT LEEQDRVMEG LFHVIKQEIG V
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the biosynthesis of kanosamine (3-amino-3-deoxy-D-glucose), which is known to have antibiotic and antifungal properties, and to be a precursor of the antibiotic neotrehalosadiamine (3,3'-diamino-3,3'-dideoxy-alpha,beta-trehalose (NTD)). Catalyzes the reversible pyridoxal phosphate-dependent transamination of 3-dehydro-alpha-D-glucose 6-phosphate to form alpha-D-kanosamine-6-phosphate. It can only use alpha-anomer and glutamate is the only amino donor.
Gene References into Functions
  1. The structure of NtdA, a sugar aminotransferase involved in the kanosamine biosynthetic pathway in Bacillus subtilis, reveals a new subclass of aminotransferases. PMID: 24097983
Protein Families
DegT/DnrJ/EryC1 family
Database Links
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