Recombinant Bacillus subtilis Probable A/G-specific adenine glycosylase YfhQ (yfhQ)

Code CSB-YP516276BRJ
MSDS
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Source Yeast
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Code CSB-EP516276BRJ
MSDS
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Source E.coli
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Code CSB-EP516276BRJ-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP516276BRJ
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP516276BRJ
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mutY
Uniprot No.
Alternative Names
mutY; yfhQ; BSU08630; Adenine DNA glycosylase; EC 3.2.2.31
Species
Bacillus subtilis (strain 168)
Expression Region
1-369
Target Protein Sequence
MNVLEDKLKQ KDIQQFRDDL ISWFEREQRV LPWREDQDPY KVWVSEVMLQ QTRVETVIPY FLRFVEQFPT VEALADADEE KVLKAWEGLG YYSRVRNLQS AVKEVKQEYG GIVPPDEKDF GGLKGVGPYT KGAVLSIAYN KPIPAVDGNV MRVMSRILSI WDDIAKPKTR TIFEDAIRAF ISKEKPSEFN QGLMELGALI CTPKSPSCLL CPVQQHCSAF EEGTERELPV KSKKKKPGIK TMAAIVLTDE DGQVYIHKRP SKGLLANLWE FPNLETQKGI KTEREQLIAF LENEYGIQAD ISDLQGVVEH VFTHLVWNIS VFFGKVKQVS DTSKLKKVTK EELEQFAFPV SHQKIWKMAG EAAAISAAP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Base excision repair (BER) glycosylase that initiates repair of A:oxoG to C:G by removing the inappropriately paired adenine base from the DNA backbone, generating an abasic site product. 8-oxoguanine (oxoG) is a genotoxic DNA lesion resulting from oxidation of guanine; this residue is misread by replicative DNA polymerases, that insert adenine instead of cytosine opposite the oxidized damaged base. Shows a powerful dicrimination of A versus C, since it does not cleave cytosine in oxoG:C pairs. May also be able to remove adenine from A:G mispairs, although this activity may not be physiologically relevant.
Protein Families
Nth/MutY family
Database Links
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