Recombinant Bacillus subtilis Stage 0 sporulation protein J (spo0J)

Code CSB-YP328624BRJ
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP328624BRJ
MSDS
Size Pls inquire
Source E.coli
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Code CSB-EP328624BRJ-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP328624BRJ
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP328624BRJ
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
spo0J
Uniprot No.
Alternative Names
spo0J; BSU40960; Stage 0 sporulation protein J
Species
Bacillus subtilis (strain 168)
Expression Region
1-282
Target Protein Sequence
MAKGLGKGIN ALFNQVDLSE ETVEEIKIAD LRPNPYQPRK HFDDEALAEL KESVLQHGIL QPLIVRKSLK GYDIVAGERR FRAAKLAGLD TVPAIVRELS EALMREIALL ENLQREDLSP LEEAQAYDSL LKHLDLTQEQ LAKRLGKSRP HIANHLRLLT LPENIQQLIA EGTLSMGHGR TLLGLKNKNK LEPLVQKVIA EQLNVRQLEQ LIQQLNQNVP RETKKKEPVK DAVLKERESY LQNYFGTTVN IKRQKKKGKI EIEFFSNEDL DRILELLSER ES
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for the initiation of sporulation and for normal chromosome segregation. Antagonizes sporulation inhibition by Soj. It probably interacts with a specific DNA site and other proteins involved in partitioning and cell division, and antagonizes Soj in response to cell cycle events related to chromosome partitioning.
Gene References into Functions
  1. A network of cis and trans interactions is required for ParB (Spo0J) spreading. PMID: 28407103
  2. Targeting of Smc/ScpAB to ParB/parS loading sites is strictly dependent on engagement of Smc head domains and relies on an open organization of the Smc coiled coils. PMID: 26904953
  3. Non-specific binding of DNA by ParB leads to condensation. PMID: 25572315
  4. Findings indicate that spreading is important for in vivo function of Spo0J. PMID: 17462018
  5. Study demonstrates that structural maintenance of chromosomes is highly enriched in the region around the origin of replication, specifically near parS sites to which Spo0J binds and at highly transcribed genes. PMID: 19450516
  6. In Bacillus subtilis, parS sites are bound by the partitioning protein Spo0J (ParB), and we show here that Spo0J recruits the structural maintenance of chromosomes complex to the origin. PMID: 19450517

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Protein Families
ParB family
Database Links
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