Recombinant Bacillus subtilis Uncharacterized protein ymdB (ymdB)

Code CSB-YP523306BRJ
MSDS
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Source Yeast
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Code CSB-EP523306BRJ
MSDS
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Source E.coli
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Code CSB-EP523306BRJ-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP523306BRJ
MSDS
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Source Baculovirus
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Code CSB-MP523306BRJ
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ymdB
Uniprot No.
Alternative Names
ymdB; BSU16970; 2',3'-cyclic-nucleotide 2'-phosphodiesterase; EC 3.1.4.16; Global regulator YmdB
Species
Bacillus subtilis (strain 168)
Expression Region
1-264
Target Protein Sequence
MRILFIGDVV GSPGRDMVKE YVPKLKTKYK PHFTIINGEN AAHGKGLTEK IYHSLIQSGA DAITMGNHTW DKKEIFDFID DVPNLVRPAN FPEGTPGKGI TYVKANGKEL AVINLQGRTF LPPLDDPFLK ADELIAEAAK RTPYIFIDFH AEATSEKLAL GWYTDGRASA VVGTHTHVQT ADNRILPKGT AYITDVGMTG PYDGILGMDR ETIIKRFKTN LPVRFTVAEG KTTLSGVVID IDDQTKKAVK IERILINDDH MFFE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays a central, regulatory role in the late adaptive responses and affects the levels of many genes. May act via regulation of cAMP levels. Decreases the expression of motility genes and induces genes involved in biofilm formation, by controlling the expression of SlrR. Required for formation of intercellular nanotubes that bridge neighboring cells to allow molecular exchange. Plays a key role in directing the early stages of colony development. In vitro, has a metal-dependent phosphodiesterase activity against 2',3'-cAMP and 2',3'-cGMP. Has also 3',5'-cyclic-nucleotide phosphodiesterase activity, but cannot use cyclic di-AMP or cyclic di-GMP, and does not have phosphatase activity.
Gene References into Functions
  1. Authors find that YmdB is a phosphodiesterase with activity against 2',3'- and 3',5'-cyclic nucleotide monophosphates. PMID: 24163345
  2. T he ymdB mutant is unable to form biofilms. PMID: 21856853
Subcellular Location
Cytoplasm.
Protein Families
YmdB-like family
Database Links
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