Recombinant Betaine reductase complex component B subunit alpha (grdI)

Code CSB-YP524802EOV
MSDS
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Source Yeast
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Code CSB-EP524802EOV
MSDS
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Source E.coli
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Code CSB-EP524802EOV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP524802EOV
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP524802EOV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
grdI
Uniprot No.
Alternative Names
grdIBetaine reductase complex component B subunit alpha; EC 1.21.4.4; Selenoprotein PB alpha
Species
Peptoclostridium acidaminophilum (Eubacterium acidaminophilum)
Expression Region
1-442
Target Protein Sequence
MKLELGNFYV EEIVFGEKTS FKDGVLTINK QEALDYVMED ENITHAELHI VKPGDMVRLC PVKEAIEPRI KLDGRTYFPG VTDEELTRCG EGRTHALKGC SVLVVGKHWG GFQDGLIDMG GEGAKYTYYS TLKNIVLVGD TNEDFEKNEQ QKKNKALRWA GHKLAEYIGK TVKDMEPQEV ETYELEPVTQ RSEEVTKLPG VVFVMQPQSQ MEELGYNDMV YGWDMNRMVP TYMHPNEVLD GAIISGSFMP CSSKWSTYDF QNFPALKRLY AEHGKTVNFL GVIMSNLNVA LQQKQRSALF VAQMAKSLGA QGAIVAEEGY GNPDADFIAC IVALENEGIK TVGLTNECTG RDGFSQPLVT LDEKANAIVS CGNVSELVEL PPMPVVLGEL EALARDGLSG GWAGDEILGS SVKADGSVIM ENNAMFCGDQ VVGWSTKTMK EF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
In the first step of betaine reductase, the substrate is bound to component PB via a Schiff base intermediate. Then the PB-activated substrate is nucleophilically attacked by the selenol anion of component PA to transform it to a carboxymethylated selenoether and the respective amine. By action of component PC, acetyl phosphate is formed, leaving component PA in its oxidized state. Finally component PA becomes reduced by the thioredoxin system to start a new catalytic cycle of reductive deamination.
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