Recombinant Biofilm regulatory protein A (brpA)

Code CSB-YP813586FMR
MSDS
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Source Yeast
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Code CSB-EP813586FMR
MSDS
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Source E.coli
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Code CSB-EP813586FMR-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP813586FMR
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP813586FMR
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
brpA
Uniprot No.
Alternative Names
brpA; SMU_410Biofilm regulatory protein A
Species
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Expression Region
27-406
Target Protein Sequence
TSIY NFSLGEFSKT FKDYGTGSGK DVIADEKPFS ILLMGVDTGS SERTSKWEGN SDSMILVTVN PKTKKTTMTS LERDILVKLS GSKTNDQTGY DAKLNAAYAA GGAKMAIMTV QDMLDIKIDK YVQINMEGLV QLVDAVGGIT VTNHFDFPIS IEEHEPEFTA SVEPGTHKIN GEQALVYSRM RYDDPDGDYG RQKRQREVIS KVLKKILALD SVSKYRKILS AVSKNMQTNI EISSSTIPKL LGYSDALKSI RTYQLKGEGT TIDGGSYQLV TSKELLKAQN RIKGQLGLKK STAENLKTTA SLYENFYGGD TSIYDSSSSA SDYSSSGNYS GSSSDYGSSS SYGSNSSSGS SSDYSGQNSY NQGNYQQPAA GTGIGN
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in biofilm formation, cell division, autolysis and the regulation of acid and oxidative stress tolerance. May be associated with systemic virulence in blood.
Gene References into Functions
  1. Expression of BrpA in Streptococcus mutans is regulated by FNR-box mediated repression.brpA expression in S. mutans is auto-regulated through the upstream brpBA promoter. PMID: 28744965
  2. RgpG in S. mutans plays a critical role in cell division and biofilm formation and that BrpA and Psr may be responsible for attachment of cell wall antigens to the cell envelope. PMID: 28687645
  3. loss of BrpA can dramatically influence the transcriptome and significantly affects the regulation of acid and oxidative stress tolerance and biofilm formation in S. mutans, which are key virulence attributes of the organism. PMID: 16585759
Subcellular Location
Cell envelope.
Protein Families
LytR/CpsA/Psr (LCP) family
Database Links

KEGG: smu:SMU_410

STRING: 210007.SMU_410

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