Recombinant Bothropoides pauloensis Zinc metalloproteinase neuwiedase

Code CSB-YP872779BUR
MSDS
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Source Yeast
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Code CSB-EP872779BUR
MSDS
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Source E.coli
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Code CSB-EP872779BUR-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP872779BUR
MSDS
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Source Baculovirus
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Code CSB-MP872779BUR
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Uniprot No.
Alternative Names
Snake venom metalloproteinase neuwiedase; SVMP; EC 3.4.24.-; Fragment
Species
Bothrops pauloensis (Neuwied's lancehead) (Bothrops neuwiedi pauloensis)
Expression Region
1-198
Target Protein Sequence
QQRFFPQRYI ELVIVADRRM YTKYNSDSNK IRTRVHELVN TVNGFFRSMN VDASLANLEV WSKKDLIKVE KDSSKTLTSF GEWRERDLLR RKSHDNAQLL TAIDFNGNTI GRAYLGSMCN PKRSVGIVQD HSPINLLVGV TMAHELGHNL GMEHDGKDCL CGASLCIMSP GLTDGPSYEF SDCSKDYYQT FLTNHNPQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
This non-hemorrhagic metalloprotease hydrolyzes the Aalpha chain of fibrin and fibrinogen first followed by the Bbeta chain and shows no effect on the gamma chain. It is also able to degrade type I collagen, fibronectin, laminin and induces inflammatory reaction. It is devoid of hemorrhagic and thrombotic activities, but induces pulmonary bleeding. It also induces a mild myotoxic reaction. It is not able to inhibit platelet aggregation, but it induces decrease of platelets and plasma fibrinogen. It contributes to local tissue damage by inducing edema, inflammatory infiltrate and mild myotoxicity, and by degrading extracellular matrix components. Cleaves insulin B chain at '38-Ala-|-Leu-39' and '40-Tyr-|-Leu-41' bonds.
Subcellular Location
Secreted.
Protein Families
Venom metalloproteinase (M12B) family, P-I subfamily
Tissue Specificity
Expressed by the venom gland.
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