Recombinant Bovine A-kinase anchor protein 5 (AKAP5)

Code CSB-YP001527BO
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP001527BO
MSDS
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Source E.coli
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Code CSB-EP001527BO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001527BO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP001527BO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AKAP5
Uniprot No.
Alternative Names
AKAP5; AKAP75A-kinase anchor protein 5; AKAP-5; A-kinase anchor protein 75 kDa; AKAP 75; P75; cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein
Species
Bos taurus (Bovine)
Expression Region
1-428
Target Protein Sequence
MEITVSEIQV ESKDETRSAE VRPQDERQEE KASMLCFKRR KKAAKAMKPK ASSKAADAAK KCPPEARASD QPQRPGGAWD SIKRLVTRRK RSESSKQQKP FKAKLQSEIN AEDANPSKKK AKSRLKIPCI KFSKGEKRSN HSKIIEDSDR SVKVQEAENL VTKTQTQSDD QATKSKSPQD VREDVSQKGD DEVCESNVNN SITSPGEKVI SVELELDMGH SAIQRGTLIL EKDTEMLEEK QSIQPQHVSP LEASDTEQEL PVGSEVPPSS AVPDQQILEE ARNGVLESGP DWKEHESREI VVEESKPKDT ELSQELDFQE NEITAEKPKP EESKRMEPIA IIITDTEISE FDVKKSKNVP KPFLISIENE QVGVFANDSG FEGRTSEQYE TLLIETASSL VKNAIQLSIE QLVNEMASDD NTINNRLQ
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Multivalent scaffold protein that anchors the cAMP-dependent protein kinase/PKA to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Association with the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade. Plays a role in long term synaptic potentiation by regulating protein trafficking from the dendritic recycling endosomes to the plasma membrane and controlling both structural and functional plasticity at excitatory synapses.
Gene References into Functions
  1. the C-helix of protein kinase A inhibitory regulatory subunit is identified as a highly dynamic switch which relays cyclic AMP binding to the helical catalytic-subunit binding regions PMID: 16322564
Subcellular Location
Postsynaptic recycling endosome membrane; Lipid-anchor.
Tissue Specificity
Predominantly in brain, and to a lesser extent in adrenal medulla, lung and anterior pituitary.
Database Links

KEGG: bta:281612

STRING: 9913.ENSBTAP00000012705

UniGene: Bt.372

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